FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982609
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTGTGATAACTCACCGTACACTTTTGGCCAGGGGA35020.3563981196996974No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26970.2744733663135591No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24630.2506592143975885No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24530.24964151559776063No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23060.2346813432402919No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21960.22348665644218607No Hit
ACATGGAACTGAGCGGCCTGAGATCGGAAGACTCGGCCATGTATTTTTGT21780.221654798602496No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19550.19896011536633595No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18710.19041144544778238No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17650.17962383816960767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16840.17138047789100241No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16690.1698539296912607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16660.16954862005131235No Hit
GTTCCATGTAGGCTGTCCTAGTGATTTTGTCCGCAGTGATCGTGACTCTGCCCTGGAACTTCTGTGCGTAGTCTC16440.16730968269169122No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16390.16680083329177728No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16300.16588490437193226No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16300.16588490437193226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.16496897545208725No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16210.16496897545208725No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15860.16140702965268994No Hit
GTGTATTACTGTCAGCAGTGTGATAACTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15810.16089818025277602No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGAGATGACTTCTTAGTCTGGTACC15790.16069464049281046No Hit
ATCTGGGGCTGAGGTGAAGAAACCTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTTCTGGAGGCATCTTCAGCAG15120.15387605853396416No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15050.1531636693740847No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14960.1522477404542397No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14870.15133181153439468No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14790.15051765249453242No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14780.15041588261454963No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCT14690.14949995369470462No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14540.1479734054949629No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14370.14624331753525563No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14330.1458362380153245No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14170.14420791993560003No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13470.13708402833680539No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13460.1369822584568226No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13380.13616809941696034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13270.1350486307371498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13220.13453978133723588No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13190.13423447169728753No Hit
GTATTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13140.13372562229737361No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12920.13148668493775245No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12730.12955305721807964No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12190.12405748369900949No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12070.12283624513921611No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12030.12242916561928498No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11910.12120792705949163No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11900.12110615717950883No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11800.12008845837968103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11720.11927429933981878No Hit
GGATTATTCCTATGCTTGGTAAAGGAGACTACGCACAGAAGTTCCAGGGC11640.11846014029995655No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11640.11846014029995655No Hit
GTGTGATAACTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11480.11683182222023206No Hit
GCCTACATGGAACTGAGCGGCCTGAGATCGGAAGACTCGGCCATGTATTTTTGTGCGATAGAACCTAATGTCCGA11380.11581412342040426No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11190.11388049570073142No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11170.11367695594076586No Hit
GGCCAAAAGTGTACGGTGAGTTATCACACTGCTGACAGTAATACACTGCA11050.11245571738097249No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11040.1123539475009897No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10810.11001324026138576No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10710.10899554146155796No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10580.10767253302178181No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10500.10685837398191957No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10380.10563713542212619No Hit
GTTATCACACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT10340.10523005590219509No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC10270.10451766674231561No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10200.10380527758243614No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10140.10319465830253946No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10130.10309288842255669No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10080.10258403902264279No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9920.10095572094291828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT200.006600078753.19348554
GGTATCA6450.044.6261331
TCGTATG1600.040.92502240
GTATCAA16550.039.0809751
ATCTCCG2000.038.9408810
GTACGGA350.001477290838.69901314
CTTATAC1850.038.439491
GACTGGG11650.036.41777870
CGTATGC1900.036.3447941
TTATACA2150.034.650882
TGTTAGA6800.034.21508870
GCCGTCT1950.033.9380846
CACGAGA2300.033.87025521
GTACTGA502.07645E-433.8633581
TATACAC2200.033.8633583
CGGGTAC701.1264765E-633.86163717
CGGGATT400.002845098733.86163717
GTATGCC2050.033.7519742
AACTGTG16550.033.49150570
TATCAAC19450.033.253992
Produced by FastQC (version 0.11.5)