FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008115
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA47830.47444983955203524No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45740.45371807779866385No Hit
GAAGTAATCAGCCTCATCTTCCGACCGGAGGCCACTGATGGCCAGGGAGG42490.42147969229700977No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTC40260.39935920009125947No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38510.38200006943652265No Hit
GTACCAACACCTCCCAGGAGCGGCCCCCAAACTCCTCTTGTATAGTAATGATCAGCGGCCCTCAGGGGTCTCTGA35940.3565069461321377No Hit
GATTACTTCTGTGCATCTTGGCATGACAGCCGGAACGCCGATTATGTCTTCGGCACTGGGACCACGGTCACCGTC35550.3526383398719392No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34140.338651840315837No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC32150.3189120288855934No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCT30550.3030408237155483No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30440.3019496783601077No Hit
GTGTTGGTACCAATCAACGGTATTAGTTCCGATGTCGGAGCTGGCCCCAG29940.29698992674446867No Hit
GAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCCCAAA29470.2923277602257679No Hit
TTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGGTACCA29350.2911374198380145No Hit
GTATTAGTTCCGATGTCGGAGCTGGCCCCAGAACAGGAGATGGTGACCCT28970.2873680086101288No Hit
GTATAGTAATGATCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC28620.2838961824791814No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27790.27566299479722056No Hit
GTAATGATCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAG27260.2704056580846431No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCGTGGTC26900.266834636921383No Hit
GATCATTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGG25750.2554272082054131No Hit
CTCCTGTTCTGGGGCCAGCTCCGACATCGGAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCC24900.2469956304588266No Hit
CCCCTGAGGGCCGCTGATCATTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGGTACCAATCAA24250.24054795335849583No Hit
CTCCTGGGAGGTGTTGGTACCAATCAACGGTATTAGTTCCGATGTCGGAGCTGGCCCCAGAACAGGAGATGGTGA23610.23419947129047777No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23410.23221557064422213No Hit
GTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC23130.22943810973946427No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23010.22824776935171084No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22440.22259365250988228No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT21670.2149556350217981No Hit
CTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC21530.21356690456941918No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21260.21088863869697405No Hit
GCCTTGGGCTGACGGAGGACGGTGACCGTGGTCCCAGTGCCGAAGACATA21030.20860715295378007No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20610.20444096159664324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20550.20384579140276657No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20180.20017557520719365No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19840.19680294410855903No Hit
CCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGG19620.19462065339767784No Hit
TCCCAGGAGCGGCCCCCAAACTCCTCTTGTATAGTAATGATCAGCGGCCC19360.19204158255754553No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGGGGCCAGCT19060.18906573158816206No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18760.18608988061877862No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18720.18569310048952747No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18430.18281644455245682No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18390.1824196644232057No Hit
GAACAGGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACACTGAGGGTGG18370.18222127435858013No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17870.17726152274294105No Hit
GGCCATCAGTGGCCTCCGGTCGGAAGATGAGGCTGATTACTTCTGTGCAT17620.1747816469351215No Hit
GGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17200.17061545557798466No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17040.16902833506098014No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16950.1681355797701651No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16780.16644926422084783No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC16540.16406858344534106No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16400.16267985299296212No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15230.15107403421236665No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCGGAAGATG15090.1496853037599877No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14570.14452716207972305No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14420.14303923659503132No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14300.14184889620727795No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14020.13907143530252006No Hit
CTTCTGTGCATCTTGGCATGACAGCCGGAACGCCGATTATGTCTTCGGCA13980.1386746551732689No Hit
GAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13950.13837707007633057No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGGGGCCAG13900.1378810949147667No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13760.13649236446238772No Hit
GTTCTGGGGCCAGCTCCGACATCGGAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCCCAAA13630.13520282904232156No Hit
GCACAGAAGTAATCAGCCTCATCTTCCGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG13390.1328221482668148No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13290.13183019794368697No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13200.13093744265287197No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13180.13073905258824636No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGC12940.12835837181273962No Hit
GACGGAGGACGGTGACCGTGGTCCCAGTGCCGAAGACATAATCGGCGTTC12830.12726722645729902No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12680.12577930097260728No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12580.12478735064947948No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12490.12389459535866443No Hit
GTCTCTGGTGACTCCATCAGCAGTGGGACTTTCTATTGGAGCTGGGTCCG12390.1229026450355366No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGAGACCCCTGAGGGCCGCTG12300.12200988974472159No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12280.12181149968009602No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12270.12171230464778322No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12180.1208195493569682No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11920.11824047851683586No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGG11670.11576060270901632No Hit
GTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11640.11546301761207799No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11500.11407428715969904No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11480.11387589709507348No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG11460.1136775070304479No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11380.11288394677194567No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11270.11179280141650506No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11010.10921373057637275No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10980.10891614547943439No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10940.10851936535018326No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGA10860.107725805091681No Hit
GTCAGAGACCCCTGAGGGCCGCTGATCATTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGGTA10790.10703143986549155No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10790.10703143986549155No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10720.10633707463930206No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC10550.10465075908998478No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10540.10455156405767198No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG10490.10405558889610808No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10390.10306363857298026No Hit
GCACTGGGACCACGGTCACCGTCCTCCGTCAGCCCAAGGCCAACCCCACT10340.10256766341141636No Hit
TCAGTGGCCTCCGGTCGGAAGATGAGGCTGATTACTTCTGTGCATCTTGG10310.102270078314478No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10260.1017741031529141No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10220.10137732302366298No Hit
CCGTGGTCCCAGTGCCGAAGACATAATCGGCGTTCCGGCTGTCATGCCAAGATGCACAGAAGTAATCAGCCTCAT10150.10068295779747351No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA10150.10068295779747351No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10100.1001869826359096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATGG350.004265113359.3940170
CGTATGC850.052.72560541
TATACAC1150.049.875323
TTATACA1400.045.78892
GGTATCA5600.045.1864131
CTTATAC1350.044.9855841
TCGTATG950.043.46075440
ACGAGAC1400.043.3854122
GTGGTAT1400.043.3789561
TACCGCG405.7048404E-542.173998
ATGCCGT1000.041.6266944
GTATGCC1100.040.82734342
AATCTCG1200.039.83258436
CGAGACG1450.039.56413723
TGGTATC1650.038.8511852
ATCTCGT1150.038.65763537
GTATCAA14650.038.460371
CGCTCAT1500.038.32137328
AGACGCT1500.038.26621225
CCCACGA1500.038.2396419
Produced by FastQC (version 0.11.5)