FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008115
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44960.4459808652782669No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44190.4383428477901827No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTC41130.4079891679024715No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37950.3764451476270068No Hit
GAAGTAATCAGCCTCATCTTCCGACCGGAGGCCACTGATGGCCAGGGAGG36970.36672403446035423No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33780.33508081915257687No Hit
GTACCAACACCTCCCAGGAGCGGCCCCCAAACTCCTCTTGTATAGTAATGATCAGCGGCCCTCAGGGGTCTCTGA33620.3334936986355723No Hit
GATTACTTCTGTGCATCTTGGCATGACAGCCGGAACGCCGATTATGTCTTCGGCACTGGGACCACGGTCACCGTC32900.32635165630905205No Hit
GTGTTGGTACCAATCAACGGTATTAGTTCCGATGTCGGAGCTGGCCCCAG31330.3107780362359453No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31320.31067884120363254No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCT30730.3048263342971784No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28510.28280503712374083No Hit
GTATAGTAATGATCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC27970.27744850537885063No Hit
GAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCCCAAA27770.275464604732595No Hit
GTAATGATCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAG26780.2656442965336296No Hit
GTATTAGTTCCGATGTCGGAGCTGGCCCCAGAACAGGAGATGGTGACCCT26730.2651483213720657No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGTGACCGTGGTC26730.2651483213720657No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26640.26425556608125067No Hit
TTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGGTACCA26570.2635612008550612No Hit
CCCCTGAGGGCCGCTGATCATTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGGTACCAATCAA24670.24471414471563266No Hit
CTCCTGTTCTGGGGCCAGCTCCGACATCGGAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCC24410.2421350738755003No Hit
CTCCTGGGAGGTGTTGGTACCAATCAACGGTATTAGTTCCGATGTCGGAGCTGGCCCCAGAACAGGAGATGGTGA24390.24193668381087474No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23560.23370349612891386No Hit
GTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC23230.23043006006259206No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22940.2275534041255214No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22540.22358560283301013No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22330.2215025071544417No Hit
GATCATTACTATACAAGAGGAGTTTGGGGGCCGCTCCTGGGAGGTGTTGG21920.21743551082961765No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT20840.2067224473398372No Hit
CTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20770.20602808211364776No Hit
GCCTTGGGCTGACGGAGGACGGTGACCGTGGTCCCAGTGCCGAAGACATA20310.2014651106272598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20240.20077074540107032No Hit
GGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA20020.19858845469018913No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19250.19095043720210492No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18880.187280221006532No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18810.1865858557803425No Hit
CCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGG18770.18618907565109138No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18620.18470115016639968No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGGGGCCAGCT18540.1839075899078974No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18460.18311402964939516No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18350.18202288429395455No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18240.18093173893851394No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG17990.17845186313069442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17260.17121062577186136No Hit
GGCCATCAGTGGCCTCCGGTCGGAAGATGAGGCTGATTACTTCTGTGCAT17220.1708138456426102No Hit
TCCCAGGAGCGGCCCCCAAACTCCTCTTGTATAGTAATGATCAGCGGCCC17030.16892914002866738No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16990.16853235989941623No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16800.1666476542854734No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16440.16307663312221324No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16130.160001587120517No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15870.15742251628038467No Hit
GAACAGGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACACTGAGGGTGG15390.15266115472937117No Hit
GAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15200.1507764491154283No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCGGAAGATG15110.14988369382461325No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14880.14760220808141927No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14040.1392698253671456No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13960.13847626510864336No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13640.13530202407463435No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13540.13431007375150653No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGTTCTGGGGCCAG13290.13183019794368697No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13190.13083824762055915No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13080.12974710226511854No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13030.12925112710355466No Hit
CTTCTGTGCATCTTGGCATGACAGCCGGAACGCCGATTATGTCTTCGGCA13020.12915193207124187No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGC12950.12845756684505238No Hit
GTTCTGGGGCCAGCTCCGACATCGGAACTAATACCGTTGATTGGTACCAACACCTCCCAGGAGCGGCCCCCAAA12810.12706883639267344No Hit
GCACAGAAGTAATCAGCCTCATCTTCCGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG12620.12518413077873058No Hit
GACGGAGGACGGTGACCGTGGTCCCAGTGCCGAAGACATAATCGGCGTTC12550.12448976555254113No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12400.12300184006784941No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGAGACCCCTGAGGGCCGCTG12360.12260505993859827No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12310.12210908477703437No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12300.12200988974472159No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12250.12151391458315768No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12130.12032357419540429No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12120.1202243791630915No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC11820.11724852819370807No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11790.1169509430967697No Hit
GTCTCTGGTGACTCCATCAGCAGTGGGACTTTCTATTGGAGCTGGGTCCG11750.11655416296751858No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11670.11576060270901632No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11660.11566140767670355No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11640.11546301761207799No Hit
GTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11410.11318153186888401No Hit
GGGTCACCATCTCCTGTTCTGGGGCCAGCTCCGACATCGGAACTAATACC11280.11189199644881785No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG11080.10990809580256221No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11030.10941212064099831No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGG11000.10911453554405995No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10930.10842017031787049No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10890.10802339018861934No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGTGTCTGGGA10770.10683304980086597No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10670.10584109947773816No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGG10610.10524592928386146No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10600.10514673425154869No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10590.1050475392192359No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC10490.10405558889610808No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGGAGGACGGT10460.10375800379916975No Hit
GGGTTGGCCTTGGGCTGACGGAGGACGGTGACCGTGGTCCCAGTGCCGAA10130.10048456773284795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1950.051.828861
GTACACG200.00808934150.525624
TGGTATC2500.041.767852
GGTATCA6600.039.3035551
TAGGTTG350.001515306538.4995275
GTATCAA15950.033.7943651
GCTTGTA701.1724987E-633.688761
TATCAAC16600.032.0604362
GAGTTTA1400.031.282421
TTACCTG450.00467082230.60137440
TCAACGC18300.029.082144
GACAGGT1302.6011548E-1028.5058731
CAACGCA18800.028.311495
AACGCAG19100.028.0501376
AGTTTAT1603.6379788E-1227.3680442
AATGGTA3700.026.79136570
CGCAGAG20250.026.4702998
ACGCAGA20350.026.332387
AGAGTAC20300.026.2442411
GCCAGAG22050.026.01027770
Produced by FastQC (version 0.11.5)