FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516383
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCGGATGTCAGGCTGTTGATTTCAAGATACA45650.3010453163877464No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43860.2892409107725423No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40490.2670169739439179No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36270.23918759310807364No Hit
GTTTATTACTGTGCGCGAGCGGCCGGTGTTACTATGCTTCGGGGATTTGTTATAACCCACTTTGACTACTGGGGC35970.23720920110552546No Hit
TAGTAACACCGGCCGCTCGCGCACAGTAATAAACAGCCGTGTCGTCGGAT31290.20634628586577403No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30570.2015981450596584No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30540.20140030585940358No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28810.18999157864470914No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT28560.18834291864258568No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAATACCACCCCTCCAGTAGTCAGGCTGCTATCC28050.1849796522382538No Hit
GTGTTACTATGCTTCGGGGATTTGTTATAACCCACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCT27050.1783850122297599No Hit
CTCCTGCACTGGAGGCAGTTCCAATATTGGGAATAGTTATGTATCCTGGTACCAGCAAGTCCCGGGAACAGCCCC26940.17765960182882556No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26700.17607688822678702No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25990.17139469382075637No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25770.16994387301888772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25420.16763574901591483No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAATACCACCCCTCCAGTA24240.15985407380589206No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23690.15622702180122042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22890.15095130979442528No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22790.1502918457935759No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22120.145873436987885No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22100.1457415441877151No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG22010.14514802658695064No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21300.14046583218092No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21180.13967447537990071No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21090.13908095777913626No Hit
CTACTGGAGGGGTGGTATTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA20480.135058227373955No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20330.13406903137268092No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20010.13195874656996287No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19950.13156306816945323No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19830.13077171136843396No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19610.12932089056656532No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGAGGCAGTTCCAATATTGGGAA19600.12925494416648037No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19590.12918899776639542No Hit
GATTATTACTGCGGAGCGTGGGATAGCAGCCTGACTACTGGAGGGGTGGT19440.12819980176512136No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19060.12569383856189367No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18640.12292408975832622No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18060.11909919855339977No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18030.11890135935314496No Hit
GTTATGTATCCTGGTACCAGCAAGTCCCGGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCT17920.11817594895221062No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17690.11665918175025702No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCCGTGGCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17460.11514241454830343No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17300.1140872721469444No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAAATGGGGCTCCGCTGGG17290.11402132574685946No Hit
GTATTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC16970.11191104094414141No Hit
GCCGAATACCACCCCTCCAGTAGTCAGGCTGCTATCCCACGCTCCGCAGT16930.11164725534380167No Hit
GGATAGCAGCCTGACTACTGGAGGGGTGGTATTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16920.11158130894371673No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16820.11092184494286735No Hit
CAGTAATAAACAGCCGTGTCGTCGGATGTCAGGCTGTTGATTTCAAGATA16640.10973480974133844No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16540.10907534574048905No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16300.1074926321384505No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16210.10689911453768605No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16150.10650343613717642No Hit
GCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT16130.10637154333700656No Hit
GTTTGGGGGCTGTTCCCGGGACTTGCTGGTACCAGGATACATAACTATTC15860.1045909905347132No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15510.10228286653174033No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGG15410.10162340253089094No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC15300.1008979921299566No Hit
CTCCAGTAGTCAGGCTGCTATCCCACGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC15270.10070015292970179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG354.3619366E-968.5943736
GTATCGC501.1859811E-954.8280435
AGTATCG501.196895E-954.76793734
GGTATCA15850.046.0888251
TCTGGAA13100.043.94318470
ATCGCGT551.5258593E-743.70187837
TATCTCG2000.042.8714836
CTTATAC3150.040.9882661
CCTAACG504.2689644E-640.7725372
GTATCAA41900.039.0047531
ATCTCGT2100.037.607437
CGTATGC2050.037.11310641
CTAACGC558.212823E-637.065943
TGGTATC4450.036.649472
TCGTATG1800.036.4293440
TCTCGTA1900.036.19869238
TCTCCGA3200.036.10187511
TATCAAC46400.035.148742
GCGTATG400.00259215634.51200540
CCCACGA3250.034.5043919
Produced by FastQC (version 0.11.5)