FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516383
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42000.2769748803567436No Hit
GTAATAAACAGCCGTGTCGTCGGATGTCAGGCTGTTGATTTCAAGATACA38190.2518493019243819No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37080.24452925151495367No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35960.2371432547054405No Hit
GTTTATTACTGTGCGCGAGCGGCCGGTGTTACTATGCTTCGGGGATTTGTTATAACCCACTTTGACTACTGGGGC34650.2285042762943135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30410.20054300265829939No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAATACCACCCCTCCAGTAGTCAGGCTGCTATCC29870.19698189705371266No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28750.1895959002441995No Hit
GTGTTACTATGCTTCGGGGATTTGTTATAACCCACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCT28690.18920022184368987No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG28110.18537533063876344No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27740.18293531383562067No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27690.18260558183519598No Hit
TAGTAACACCGGCCGCTCGCGCACAGTAATAAACAGCCGTGTCGTCGGAT27390.18062718983264783No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26210.172845514622625No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25250.1665146602144709No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25140.16578924981353657No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAATACCACCCCTCCAGTA23820.1570843250023246No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT23660.1560291826009656No Hit
CTCCTGCACTGGAGGCAGTTCCAATATTGGGAATAGTTATGTATCCTGGTACCAGCAAGTCCCGGGAACAGCCCC23020.1518086129955295No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC22960.15141293459501987No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22850.15068752419408554No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22120.145873436987885No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20830.13736635137692788No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19760.1303100865678394No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19440.12819980176512136No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19020.1254300529615539No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19000.12529816016138404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18950.12496842816095934No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18710.1233857145589208No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18660.1230559825584961No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18000.11870352015289014No Hit
CTACTGGAGGGGTGGTATTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17670.11652728895008715No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17670.11652728895008715No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCCGTGGCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17570.11586782494923775No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17480.11527430734847331No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGAGGCAGTTCCAATATTGGGAA17270.11388943294668959No Hit
GTTATGTATCCTGGTACCAGCAAGTCCCGGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCT17040.112372665744736No Hit
GATTATTACTGCGGAGCGTGGGATAGCAGCCTGACTACTGGAGGGGTGGT16980.11197698734422637No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16880.11131752334337695No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16760.11052616654235771No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG16390.10808614973921496No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16360.10788831053896014No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16340.10775641773879026No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16270.10729479293819569No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15990.10544829373581739No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15970.10531640093564754No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15750.10386558013377888No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15450.1018871881312307No Hit
GTTCCAATATTGGGAATAGTTATGTATCCTGGTACCAGCAAGTCCCGGGA15350.10122772413038131No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15350.10122772413038131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)