FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527815
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTCCGCCGAATACCAC109930.7195242879537117No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC105320.6893504776429084No Hit
CTCTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG71840.4702139984225839No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACGCATTGCCAAAG51700.33839175554631945No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTC50430.3300792307969224No Hit
ATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT48440.3170540935911743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48080.3146977873629988No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47650.3118833104793447No Hit
GTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCG44410.2906765544257649No Hit
GTCTTTATATATCACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACC42880.2806622529560189No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42410.27758596426923415No Hit
CTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGT39390.2578191731328728No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC38820.25408835493826154No Hit
GATATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGG35860.234714281506596No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35660.23340522249094292No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACG35190.23032893380415823No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34800.22777626872363474No Hit
GGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC34770.22757990987128676No Hit
CTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT33840.22149278544849998No Hit
GTCTGAGACCTGAGGACACGGCCTTGTATTACTGTGCAAAAGATCCCCAT33640.22018372643284692No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33530.21946374397423773No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32930.2155365669272785No Hit
GACTATATCTGTCAATCAGCCGAAAGCAGTGGTCCTTCCGTGGTATTCGG32360.21180574873266725No Hit
GTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTCCATTTGCAGATAGA31950.20912217775057845No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31600.20683132447318556No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30930.20244597677074777No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30770.20139872955822533No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACGC29550.19341346956274155No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28990.18974810431891295No Hit
CCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCT28210.18464277415786598No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27660.18104286186482002No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27470.1797992557999496No Hit
ATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTT26810.17547936104829445No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26780.1752830021959465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26540.17371213137716282No Hit
GAGTTGGGACTGACCTGGATTTTCCTTTTGCTTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT26510.17351577252481484No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26290.17207580760759647No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26110.17089765449350872No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26010.17024312498568217No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25630.16775591285594132No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25180.1648105300707219No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25130.16448326531680865No Hit
CACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT24540.16062154122063207No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24540.16062154122063207No Hit
GTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTG24070.15754525253384735No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23560.154207152043932No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGC23400.15315990483140957No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGATTTTC23120.15132722220949527No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22990.15047633384932074No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT22840.14949453958758094No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22410.14668006270392686No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22300.14596008024531765No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC21550.14105110893661862No Hit
GTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCCGAAAGCAGTGGTCCTTCCGTGGTATTCGGCGGA21480.14059293828114006No Hit
GGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGG21100.13810572615139924No Hit
CAGTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTCCATTTGCAGATA20780.1360112317263543No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20150.13188769582704712No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20060.13129861927000325No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCC19580.12815687763243586No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19540.12789506582930524No Hit
TCCCGGAGCCGTGCACTACTTTGACCATTGGGGGCAGGGAACCCTGGTCGCCGTCTCCTCAGCATCCCCGACCAG19280.12619328910895625No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19260.12606238320739097No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19170.12547330665034706No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19100.1250151359948685No Hit
GTCTCAGACTGTCCATTTGCAGATAGAGGGAGTTCTTGGCGTTGTCTCTG19080.12488423009330317No Hit
GTAGTAGCAGAGCCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC19020.12449151238860726No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCC18950.12403334173312869No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18920.12383698288078071No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT18780.12292064156982356No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18640.12200430025886641No Hit
GCTTGGTCCGTCCGCCGAATACCACGGAAGGACCACTGCTTTCGGCTGATTGACAGATATAGTCAGCCTCGTCTT18530.12128431780025722No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18510.12115341189869193No Hit
CCACTGATGGTCAACGTGGCTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCG18440.12069524124321335No Hit
GCTCTGGAGACGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAG18390.12036797648930007No Hit
GTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTTTGACCATTGGGGGCAGGGAACCCTGGTCGC18300.1197788999322562No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18220.11925527632599497No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18020.1179462173103419No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCACATATTAGTTGGGATAGTAGT18020.1179462173103419No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18000.11781531140877659No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17970.11761895255642864No Hit
GCCTTGTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTT17660.11558991108216636No Hit
GCTCCGGGATGGGGATCTTTTGCACAGTAATACAAGGCCGTGTCCTCAGG17520.11467356977120921No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17260.11297179305086022No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17240.1128408871492949No Hit
GACCAAGCTGACCGTCGTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTC17190.11251362239538164No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTCCGCCGAATACCACGGAAGGAC17030.11146637518285919No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16990.11120456337972856No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16920.11074639272424999No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16820.11009186321642346No Hit
AGCCAGGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCC16630.10884825715155304No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16590.1085864453484224No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16560.10839008649607446No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16360.10708102748042138No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16360.10708102748042138No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT16270.1064919509233775No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16270.1064919509233775No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCCGAA16210.10609923321868157No Hit
TTGTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTTTGA16180.10590287436633362No Hit
CCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGC16140.10564106256320302No Hit
GCTCAGGGACAACAGCCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAG16080.10524834485850709No Hit
ATACAAGGCCGTGTCCTCAGGTCTCAGACTGTCCATTTGCAGATAGAGGG16080.10524834485850709No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16050.10505198600615911No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15990.10465926830146322No Hit
GGCCTGGAGTGGGTCTCACATATTAGTTGGGATAGTAGTAGCAGAGCCTA15960.10446290944911524No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGA15910.10413564469520196No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15890.10400473879363667No Hit
CTCCAGAGACAACGCCAAGAACTCCCTCTATCTGCAAATGGACAGTCTGAGACCTGAGGACACGGCCTTGTATTA15770.10321930338424481No Hit
GCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATT15680.10263022682720094No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15650.10243386797485296No Hit
GGATCACCTGCTCTGGAGACGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC15560.10184479141780908No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15470.1012557148607652No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15290.10007756174667745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)