FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527815
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTCCGCCGAATACCAC104060.681103405844294No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC97810.6401953116051354No Hit
CTCTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG68650.4493345071229174No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51110.3345300314501429No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACGCATTGCCAAAG47630.31175240457777936No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45910.30049449704316294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTC44940.2941455608172455No Hit
ATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT44820.2933601254078537No Hit
GTCTTTATATATCACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACC43200.28275674738106377No Hit
GTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCG41260.270058874929229No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40460.2648226388666167No Hit
GGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC39230.2567719259203503No Hit
CTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGT38660.253041107725739No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC38660.253041107725739No Hit
GTCTGAGACCTGAGGACACGGCCTTGTATTACTGTGCAAAAGATCCCCAT36580.23942689396294708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG35000.2290853277392878No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACG33190.21723834364762748No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32940.21560201987806116No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32390.21200210758501517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32380.21193665463423256No Hit
CTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT32380.21193665463423256No Hit
GATATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGG31050.20323141218013963No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30350.19864970562535386No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29510.19315165775961096No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29420.19256258120256706No Hit
GTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTCCATTTGCAGATAGA29080.19033718087595686No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29040.1900753690728262No Hit
CCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCT28840.18876631005717315No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGACGC28320.18536275661647517No Hit
GACTATATCTGTCAATCAGCCGAAAGCAGTGGTCCTTCCGTGGTATTCGG28120.1840536976008221No Hit
GTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTG27000.17672296711316487No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26580.1739739431802934No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26370.17259943121385768No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26240.1717485428536832No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26190.17142127809976992No Hit
GAGTTGGGACTGACCTGGATTTTCCTTTTGCTTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT26170.17129037219820462No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25730.16841044236376787No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25450.16657775974185357No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25240.16520324777541784No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24560.16075244712219738No Hit
ATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTT24470.1601633705651535No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23780.15564711696115038No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23700.15512349335488917No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23620.15459986974862794No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23530.15401079319158406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23090.1511308633571473No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTACACAGGGTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT22710.14864365122740647No Hit
CACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT22520.14740004516253605No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGATTTTC22310.14602553319610032No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22030.14419285057418602No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21450.14039657942879208No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC21410.1401347676256615No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21110.13817117910218188No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGC20750.13581487287400634No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20600.13483307861226654No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20520.1343094550060053No Hit
GTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCCGAAAGCAGTGGTCCTTCCGTGGTATTCGGCGGA19800.12959684254965426No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19720.12907321894339302No Hit
GGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCCCTCAGG19580.12815687763243586No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19400.1269787245183481No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19140.1252769477979991No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCC19100.1250151359948685No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18990.12429515353625929No Hit
GCTCTGGAGACGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAG18420.12056433534164805No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18410.1204988823908654No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18360.12017161763695212No Hit
GTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTTTGACCATTGGGGGCAGGGAACCCTGGTCGC18240.11938618222756027No Hit
GTCTCAGACTGTCCATTTGCAGATAGAGGGAGTTCTTGGCGTTGTCTCTG18110.11853529386738577No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18060.11820802911347252No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17940.11742259370408066No Hit
GTAGTAGCAGAGCCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC17880.11702987599938473No Hit
CAGTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTCCATTTGCAGATA17880.11702987599938473No Hit
TCCCGGAGCCGTGCACTACTTTGACCATTGGGGGCAGGGAACCCTGGTCGCCGTCTCCTCAGCATCCCCGACCAG17870.11696442304860209No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTCCGCCGAATACCACGGAAGGAC17830.11670261124547146No Hit
GCTTGGTCCGTCCGCCGAATACCACGGAAGGACCACTGCTTTCGGCTGATTGACAGATATAGTCAGCCTCGTCTT17540.1148044756727745No Hit
GCCTTGTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTT17440.11414994616494797No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17430.11408449321416533No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT17360.11362632255868675No Hit
CCACTGATGGTCAACGTGGCTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCG17300.11323360485399082No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17200.11257907534616429No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17070.1117281869859898No Hit
GACCAAGCTGACCGTCGTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTC17070.1117281869859898No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAAAGACAGCGAGAGGCC16990.11120456337972856No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATATCTGTCAATCAGCCGAA16930.11081184567503265No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16730.10950278665937958No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCACATATTAGTTGGGATAGTAGT16580.10852099239763978No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16440.1076046510866826No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16410.10740829223433465No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16310.10675376272650812No Hit
GGATCACCTGCTCTGGAGACGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC16240.10629559207102955No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16200.10603378026789892No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCGTCTCCAGAGCAGGTGATCCTGGC16190.10596832731711628No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16180.10590287436633362No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16030.10492108010459382No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16010.10479017420302851No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15930.10426655059676729No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15840.10367747403972341No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGA15810.10348111518737543No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15780.10328475633502748No Hit
GGCCTGGAGTGGGTCTCACATATTAGTTGGGATAGTAGTAGCAGAGCCTA15660.10249932092563563No Hit
GCTCCGGGATGGGGATCTTTTGCACAGTAATACAAGGCCGTGTCCTCAGG15490.10138662076233053No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGACGACGGTCAGCTTGGTCCGTCCGCCGAATAC15450.1011248089591999No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15380.10066663830372133No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15340.10040482650059072No Hit
TTGTATTACTGTGCAAAAGATCCCCATCCCGGAGCCGTGCACTACTTTGA15320.1002739205990254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTTA5800.057.0764961
ATCGGTA200.007458314351.57568733
CTTTATA6500.050.904793
TTATATA6950.048.1011855
TCTTTAT7000.047.7591672
TTTATAT7150.046.7526364
GGTATCA10850.044.0364041
TATATAT7600.044.000236
TATATCA7600.043.5640878
ACGGGCT551.5840487E-743.494316
ATATATC7650.043.2708477
ATATCAC7800.042.019099
TGGTATC3500.041.9111062
GCTGATT31950.041.45166870
TATCACC8150.039.800910
TAGATAC350.001425125338.9845243
AATGGTA4650.038.2584770
TCACCAA9150.035.82776612
ATCACCA9150.035.82776611
GTATCAA24350.034.758921
Produced by FastQC (version 0.11.5)