FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419431
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACCACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT130110.9166349051133871No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC101230.7131730954164027No Hit
CCCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAGGTCGGGGTCCCT58370.41122111606693107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56630.39896268293421805No Hit
GTTTAGGGGGCTGTCCTGGTTTCTGCTGGAACCAAGCTACCTGGTTCTTATTGTTGGAGTTGTAGAAAACACT53370.37599573350166365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48180.3394317863989162No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41840.29476600130615715No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36590.2577793496126265No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGCCAGAGTGTTTTCTA35040.24685948101739358No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33610.23678502160372714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31210.21987683797239882No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30980.21825647037439652No Hit
AACCAGGACAGCCCCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAG30500.21487483364813084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29760.2096614770284713No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29540.2081115601955995No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29210.20578668494629188No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28350.1997279191450659No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28010.1973325931306277No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26970.1900057135570521No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26870.18930120590574673No Hit
GTTTATTACTGTCAGCAGTATTATACCACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAA26840.18908985361035513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25830.18197432633217114No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25570.18014260643877722No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTACCTGGTTCTTATTGTTGGAGTTG24900.1754224051750314No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGCCAGAGTGTTTTCTACAACTCCAA23180.16330487357257945No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22480.158373320013442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22090.15562574017335115No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21780.15344176645430457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21200.14935562207673356No Hit
GGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21040.148228409834645No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20990.14787615600899234No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20800.14653759147151216No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC20760.14625578841099002No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20720.1459739853504679No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGC20330.14322640551037705No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20110.14167648867750526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20040.14118333332159153No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTATACCACT20030.14111288255646098No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTTGGCCGAACGTC19600.138083499655848No Hit
GTTTTCTACAACTCCAACAATAAGAACCAGGTAGCTTGGTTCCAGCAGAA19200.13526546905062664No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18960.1335746506874938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18480.13019301396122812No Hit
GCTCATTTACTGGGCATCTACCCGGGAGGTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT18320.12906580171913956No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18070.1273045325908762No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17880.12596596805339602No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17860.12582506652313497No Hit
GTCAGGGACCCCGACCTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGGGGCTGTCCTGGTTTCTGCTGGAA17750.1250501081066991No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGCCAGAGTGTTT17660.12441605122052429No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC17510.12335928974356626No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17470.12307748668304412No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACGCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17230.12138666831991128No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17100.12047080837321435No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17090.12040035760808382No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16880.11892089154034258No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16550.11659601629103493No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGAGTGGTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGC16520.11638466399564332No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16250.11448249333711889No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16160.11384843645094408No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16100.11342573186016087No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16100.11342573186016087No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTATACCACTCCTC16090.11335528109503033No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16010.11279167497398605No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15950.11236897038320284No Hit
CTATAATACCGATCTGTGGCACAATAATACACGGCTGTGTCCTCGGATTT15950.11236897038320284No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15860.11173491349702803No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15820.1114531104365059No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15810.11138265967137535No Hit
GAACCAGGTAGCTTGGTTCCAGCAGAAACCAGGACAGCCCCCTAAACTGC15690.11053725048980893No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15590.10983274283850361No Hit
ACCCGGGAGGTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC15550.10955093977798147No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15070.10616930305171579No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14960.1053943446352799No Hit
CTGCAAGGCCAGCCAGAGTGTTTTCTACAACTCCAACAATAAGAACCAGG14710.10363307550701654No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14530.10236496173466691No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGATTCACTTTCAATACCGCCTGG14480.10201270790901425No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14420.10159000331823104No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14400.10144910178796997No Hit
ACCATCAACTGCAAGGCCAGCCAGAGTGTTTTCTACAACTCCAACAATAA14220.10018098801562034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)