FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419431
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACCACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT120850.8513974966025118No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC110280.7769310378595367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54130.38134999165158434No Hit
GTTTAGGGGGCTGTCCTGGTTTCTGCTGGAACCAAGCTACCTGGTTCTTATTGTTGGAGTTGTAGAAAACACT49660.34985849963823534No Hit
CCCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAGGTCGGGGTCCCT44490.31343545406574885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42060.29631591813902897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40730.2869459663766678No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38270.2696150781545563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34390.24228018128390882No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32450.2286127328485851No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31230.22001773950265988No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30110.21212725380803996No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGCCAGAGTGTTTTCTA28330.19958701761480482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27950.1969098885398445No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27900.19655763471419183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26540.1869763306564391No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26360.18570821688408948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25560.1800721556736467No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25430.17915629572694974No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24670.1738020375770291No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24650.17366113604676803No Hit
GTTTATTACTGTCAGCAGTATTATACCACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAA24290.1711249085020688No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23870.1681659763665863No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23440.16513659346597334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23110.16281171821666568No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTACCTGGTTCTTATTGTTGGAGTTG22910.161402702914055No Hit
AACCAGGACAGCCCCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAG22550.15886647536935575No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22550.15886647536935575No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20600.14512857616890149No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20540.1447058715781183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20060.1413242348518526No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19480.1372380904742816No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19270.13575862440654038No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGACCTCCCGGGT18810.13251788921053576No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18790.1323769876802747No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTATACCACT18650.1313906769684472No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTTGGCCGAACGTC18640.13132022620331668No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18460.13005211243096707No Hit
GGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18450.12998166166583652No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGCCAGAGTGTTTTCTACAACTCCAA18410.12969985860531438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18220.12836129406783423No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18000.12681137723496244No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17850.12575461575800442No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17200.12117531602451967No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17010.11983675148703954No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGGCCAGC16720.11779367929825402No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16670.11744142547260135No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16650.11730052394234028No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16490.11617331170025172No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGAGTGGTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGC16470.11603241016999065No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16360.11525745175355478No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16310.1149051979279021No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTATACCACTCCTC16140.11370753492068301No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16080.1132848303298998No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16070.11321437956476926No Hit
GTCAGGGACCCCGACCTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGGGGCTGTCCTGGTTTCTGCTGGAA16040.11300302726937766No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15810.11138265967137535No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15580.10976229207337307No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15420.10863507983128451No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15340.10807147371024023No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15320.10793057217997916No Hit
GTTTTCTACAACTCCAACAATAAGAACCAGGTAGCTTGGTTCCAGCAGAA15310.10786012141484863No Hit
GCTCATTTACTGGGCATCTACCCGGGAGGTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15170.10687381070302114No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15050.10602840152145472No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14970.10546479540041044No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACGCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14900.10497164004449672No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14840.1045489354537135No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14570.10264676479518905No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14550.10250586326492798No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14470.1019422571438837No Hit
TCCGAGGAGTGGTATAATACTGCTGACAGTAATAAACTGCCACATCCTCA14460.10187180637875318No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14400.10144910178796997No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14300.10074459413666462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT150.002572831767.482264
GTACGGT200.00803017450.6206133
ACGCACT351.9924511E-550.3962352
AACGCAC404.43589E-544.01239451
GGTATCA11600.041.9342961
GACAGGT1850.038.3453251
TGGTATC4250.036.5378342
GTATCAA27100.034.902261
GTATGTC400.002893185233.7470743
TATCAAC30050.031.5671772
GCGGTAA450.004097117631.43291553
ATCAACG30650.030.8293023
TCAACGC31000.030.5847024
CAACGCA31300.030.3993575
AACGCAG31750.030.075836
CCGTTCG450.00511444630.03974220
CAAACGC703.4608413E-530.03856549
ACGAACT41550.029.12186452
CGAACTG41600.029.07166953
AACGAAC42350.029.01596551
Produced by FastQC (version 0.11.5)