FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769253
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33000.42898760225829474No Hit
GTATATGAAGTGCAGTAATAGTCAGCCTCATCGTCAGCCTGGAGCCCCGA29470.38309892844096805No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCATGGTCCCCCCGCCGAACAGCAAAGTTTGGCGGGCTGTATATGAAGTG21400.2781919602523487No Hit
CTTCATATACAGCCCGCCAAACTTTGCTGTTCGGCGGGGGGACCATGCTGACCGTCCTGGGTCAGCCCAAGGCTG20930.27208213682624577No Hit
CCTATGAACACTCCGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19640.2553126214652397No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19160.24907280179602811No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18300.23789312488869072No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16940.22021363582592465No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16360.21267385372562733No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15400.2001942143872042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15010.19512436090596982No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14770.19200445107136402No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14440.18771457504878108No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14260.18537464267282677No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14240.1851146501866096No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13500.1754949281965751No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13180.17133504841710076No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCGGTCACCATCTCCTG12940.16821513858249496No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGCCGCCCTATCGCTGGACACGTCC12820.16665518366519208No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCACTGACGTT12790.16626519493586636No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12670.16470524001856346No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12450.16184532267017482No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12160.15807543162002619No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12140.15781543913380905No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11870.15430554056987753No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11770.1530055781387918No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11580.15053564951972886No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11530.149885668304186No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG11370.1478057284144488No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11230.14598578101092877No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11200.14559579228160305No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11040.14351585239186587No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10780.14013595007104296No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10740.13961596509860866No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10710.13922597636928294No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10670.13870599139684864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10520.13675604775022002No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC10510.13662605150711143No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10420.13545608531913428No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC10220.1328561604569628No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10210.13272616421385422No Hit
GACTATTACTGCACTTCATATACAGCCCGCCAAACTTTGCTGTTCGGCGGGGGGACCATGCTGACCGTCCTGGGT10170.13220617924141992No Hit
GTAATAGTCAGCCTCATCGTCAGCCTGGAGCCCCGAGACGGTCAGGGAGG10080.13103621305344273No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10030.13038623183789988No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9930.12908626940681414No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG9920.12895627316370556No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9860.12817629570505412No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCATGGTCCCCC9800.12739631824640268No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9720.12635634830153408No Hit
CTCCGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT9560.12427640841179691No Hit
GTCTCTGGTGGCTCCGTCAGCGGTAGTCATTACCACTGGGGCTGGATGCG9470.12310644222381974No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9390.12206647227895114No Hit
GGACTGGTGAGGCCCTCGGAGACCCTGTTACTCACCTGCAGTGTCTCTGGTGGCTCCGTCAGCGGTAGTCATTAC9390.12206647227895114No Hit
GTAGTGGCTGGTGGGCCTTTGACTACTGGAGCCAAGGAATTCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCC9380.12193647603584257No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAATTCCTTGGCTCCAGTAGTCAAAGGCCCACCAGC9300.12089650609097397No Hit
CCCCTGAGGGACGCTTAGTGACCGCATAAATCATGAGTCTGGGGCTTTTGCCTGGGTGCTGTTGGTACCAGGAGA9270.12050651736164825No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9200.11959654365988823No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9040.11751660377015104No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9030.11738660752704247No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8990.11686662255460817No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8980.11673662631149959No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8950.11634663758217387No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8940.1162166413390653No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8850.11504667515108813No Hit
CTATTACAACCCGTCCCTCAAGAGTCGAGCCGCCCTATCGCTGGACACGTCCGAGAACCAGTTCTCCCTGAGG8760.11387670896311096No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAATTCCTTGG8740.11361671647689381No Hit
AGCCACTACTGTATCCACAACGATACACCGCCGTGTCTGCGGCGGTCACA8730.11348672023378525No Hit
ACCCAGGACGGTCAGCATGGTCCCCCCGCCGAACAGCAAAGTTTGGCGGG8650.11244675028891665No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8530.11088679537161376No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8480.1102368141560709No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8350.10854686299565942No Hit
GACCCAGGACGGTCAGCATGGTCCCCCCGCCGAACAGCAAAGTTTGGCGGGCTGTATATGAAGTGCAGTAATAGT8330.10828687050944227No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8150.10594693813348795No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8070.10490696818861935No Hit
CTCCTGCACTGGAACCAGCACTGACGTTGGTGATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAAG7970.10360700575753361No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA7950.10334701327131646No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCC7920.10295702454199074No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGC7860.10217704708333929No Hit
ACGATACACCGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA7720.10035709967981925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTA200.00616459754.11815660
ACGAGAT200.006834988552.72405650
ATGCGAA200.00789972750.82856814
ATAGCGG403.626373E-545.53658763
GGTATCA4500.042.9191061
TGGTATC1200.042.354382
TAATCGT503.9918723E-641.16869438
GTATCAA12850.040.6074751
TCTCGTA350.001385156339.20828238
GTCCTAT350.001472411638.724011
AGCCTAT350.001472411638.724012
CGTATGC557.4276504E-637.6092741
TTAATCG557.753853E-637.37442437
ACGAGAC558.360606E-636.9662322
GTGGTAT1400.036.303761
TATCAAC14950.034.903412
TGATTTG800.002542239434.6424370
GTAGTTT800.002542239434.6424370
AATGGTA3450.034.14036670
AGTAGAC400.002835726633.8835073
Produced by FastQC (version 0.11.5)