FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050981
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGA69230.6587179026071832No Hit
CAGTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA51660.4915407604894856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49770.47355756193499216No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48770.4640426420648898No Hit
ATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT46690.444251608735077No Hit
GTTTTATATAGTTCCAACCATAAAAACCACTTAGCTTGGTACCAGCAGAA41730.3970576061793696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35000.33302219545358097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34350.3268374975380145No Hit
CTATAATATTGTTGACAGTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTC30870.2937255763900584No Hit
ATATAAAACACTCTGGCTGGACTTACAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA30550.29068080203162566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29820.283734910526451No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29690.28249797094333773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28410.27031887350960676No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28080.267178949952473No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27630.26289723601092696No Hit
CTCCCAAGCTGCTCATTTACTGGGCATCTGTTCGGGAATCCGGGGTCCCT26330.2505278401797939No Hit
GTAATATACGGCCGTGTCGTTAGATTTTAGTCTACTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT25030.23815844434866093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24930.2372069523616507No Hit
GCTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTGGTTTTTATGGTTGGAACTATATAAAACACT24530.23340098441360976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23670.22521815332532177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23510.22369576614610537No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACT23480.22341031855000232No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23460.22322002015260026No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22800.21694017303833277No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22630.21532263666041535No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22510.21418084627600312No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21590.20542711999550897No Hit
AACCAGGACAGCCTCCCAAGCTGCTCATTTACTGGGCATCTGTTCGGGAA21440.2039998820149936No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21440.2039998820149936No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21390.2035241360214885No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20730.19724428890722095No Hit
CTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGC20570.1957219017280046No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19300.18363795349297465No Hit
ATATAGTTCCAACCATAAAAACCACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGCTGCTCATTTA19190.18259131230726341No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAA18670.1776435539748102No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18380.17488422721248054No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17930.17060251327093448No Hit
GTATATTACTGTGCGCGGGGGGATATTGTAGTAGAAGCACCAGCCGGTGTTTCCTTAGGACCTGGTAGTCCCTA17920.17050736407223346No Hit
GTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17860.1699364688800273No Hit
GCTCATTTACTGGGCATCTGTTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17820.16955587208522324No Hit
GTCAGGGACCCCGGATTCCCGAACAGATGCCCAGTAAATGAGCAGCTTGGGAGGCTGTCCTGGTTTCTGCTGGTA17360.16517900894497617No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTGGTTTTTATGGTTGGAACTA17360.16517900894497617No Hit
GTAAATGAGCAGCTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA17280.16441781535536798No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17040.16213423458654344No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16810.15994580301641992No Hit
CTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAACCATAAAAACCACT16480.15680587945928615No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16300.1550931938826677No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGCC16120.1533805083060493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15880.15109692753722473No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15860.1509066291398227No Hit
CTGTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15560.148052153178792No Hit
CAGTAATATACGGCCGTGTCGTTAGATTTTAGTCTACTCAGCTCCATGTA15420.14672006439697768No Hit
GTTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGC15260.1451976772177613No Hit
TTCCCGAACAGATGCCCAGTAAATGAGCAGCTTGGGAGGCTGTCCTGGTT15080.1434849916411429No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGGCTGAAGATGTGGCGCTGTATTACTGTCAACAATATTATAGTTCT14640.13929842689829788No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14580.13872753170609173No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14450.13749059212297843No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14430.1373002937255764No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14410.13710999532817433No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14040.1335894749762365No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14000.1332088781814324No Hit
GTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAACCATAAAAACCA13920.1324476845918242No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13740.1307349990152058No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13740.1307349990152058No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13650.1298786562268966No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13640.12978350702819555No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13540.12883201504118533No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13470.12816597065027818No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13450.12797567225287612No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAA13170.1253114946892475No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13100.12464545029834032No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13060.12426485350353622No Hit
CACCATCAGCGGCCTGCAGGCTGAAGATGTGGCGCTGTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTT13060.12426485350353622No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12890.12264731712561883No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12870.12245701872821678No Hit
GACTTACAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGACTGGGTCATCACGATGTCC12870.12245701872821678No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12840.12217157113211372No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12700.12083948235029938No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12570.11960254276718608No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGG12550.11941224436978404No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12440.1183656031840728No Hit
TCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12390.11788985719056767No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12320.1172238127996605No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12060.1147499336334339No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAAC12060.1147499336334339No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11940.11360814324902163No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11900.11322754645421754No Hit
ATATTACTGTGCGCGGGGGGATATTGTAGTAGAAGCACCAGCCGGTGTTT11850.11275180046071243No Hit
GTCTGGGGCAGAGGTGAAGAACTCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTAGTGGATACACCTTCACCGG11810.11237120366590833No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGCTGCTCATTTACTGGGCATCTGTTCGGGAATCCGGGGTC11710.1114197116788981No Hit
GCCTACATGGAGCTGAGTAGACTAAAATCTAACGACACGGCCGTATATTA11700.11132456248019706No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11680.11113426408279503No Hit
GAACTATATAAAACACTCTGGCTGGACTTACAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGAGAGT11630.11065851808928992No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11050.10513986456463058No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11050.10513986456463058No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11040.10504471536592955No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11020.1048544169685275No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10990.10456896937242444No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10880.10352232818671317No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10810.10285628379580601No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10800.10276113459710498No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGAACAGATGCCCAGTAAAT10720.1019999410074968No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10680.10161934421269271No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10610.10095329982178554No Hit
ACTATATACATTGGGTGCGACAGGCCCCTGGACAAGGGCCTGAGTGGATG10520.10009695703347633No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA10510.10000180783477533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)