FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050981
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGA66860.6361675425150407No Hit
CAGTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA54230.5159941045556485No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49420.47022733998045635No Hit
ATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT45630.43416579367276853No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41640.3962012633910604No Hit
GTTTTATATAGTTCCAACCATAAAAACCACTTAGCTTGGTACCAGCAGAA38880.36994008454957794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36010.3426322645223843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35310.3359718206133127No Hit
CTATAATATTGTTGACAGTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTC31640.3010520646900372No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29970.28516214850696636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28870.2746957366498538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27790.2644196231901433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27650.26308753440832894No Hit
ATATAAAACACTCTGGCTGGACTTACAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA26690.2539532113330307No Hit
GTAATATACGGCCGTGTCGTTAGATTTTAGTCTACTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT25820.24567523104604172No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25210.23987112992527934No Hit
GCTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTGGTTTTTATGGTTGGAACTATATAAAACACT24660.23463792399672306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24370.23187859723439339No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24280.23102225444608415No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23780.22626479451103304No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23370.22236367736429108No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22930.21817711262144607No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21960.2089476403474468No Hit
CTCCCAAGCTGCTCATTTACTGGGCATCTGTTCGGGAATCCGGGGTCCCT21680.20628346278381815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21460.20419018041239567No Hit
GTAATACAGCGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21270.20238234563707622No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20930.19914727288124143No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACT20580.1958170509267056No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAAC20060.1908692925942524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20050.1907741433955514No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20010.1903935466007473No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19760.18801481663322173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19690.18734877224231455No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18880.1796416871475317No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18770.17859504596182044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18470.17574057000078974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17990.1711734084631406No Hit
AACCAGGACAGCCTCCCAAGCTGCTCATTTACTGGGCATCTGTTCGGGAA17340.16498871054757414No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTGGTTTTTATGGTTGGAACTA17340.16498871054757414No Hit
CTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGC17240.16403721856056389No Hit
ATATAGTTCCAACCATAAAAACCACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCCAAGCTGCTCATTTA17000.16175363779173932No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16520.1571864762540902No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16380.1558543874722759No Hit
GTCAGGGACCCCGGATTCCCGAACAGATGCCCAGTAAATGAGCAGCTTGGGAGGCTGTCCTGGTTTCTGCTGGTA16190.15404655269695644No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16180.15395140349825545No Hit
GTATATTACTGTGCGCGGGGGGATATTGTAGTAGAAGCACCAGCCGGTGTTTCCTTAGGACCTGGTAGTCCCTA15950.1517629719281319No Hit
GCTCATTTACTGGGCATCTGTTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15860.1509066291398227No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAA15810.15043088314631758No Hit
TCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15750.14985998795411143No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15710.14947939115930736No Hit
CTGTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15690.14928909276190533No Hit
CAGTAATATACGGCCGTGTCGTTAGATTTTAGTCTACTCAGCTCCATGTA15570.14814730237749302No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15450.14700551199308076No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15140.14405588683334905No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15030.14300924564763778No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14980.14253349965413264No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14870.1414868584684214No Hit
GTAAATGAGCAGCTTGGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14720.14005962048790607No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGGCTGAAGATGTGGCGCTGTATTACTGTCAACAATATTATAGTTCT14430.1373002937255764No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGCC14270.13577790654636002No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14180.1349215637580508No Hit
GTTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGC14120.13435066856584466No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13950.1327331321879273No Hit
CTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAACCATAAAAACCACT13830.131591341803515No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13620.12959320863079352No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13440.1278805230541751No Hit
TTCCCGAACAGATGCCCAGTAAATGAGCAGCTTGGGAGGCTGTCCTGGTT13390.12740477706067No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13210.12569209148405158No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13180.1254066438879485No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12980.12350365991392805No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12950.12321821231782495No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12930.12302791392042292No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12750.12131522834380451No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12740.12122007914510348No Hit
GTCTCTGGGCGAGAAGGCCACCATCAACTGTAAGTCCAGCCAGAGTGTTTTATATAGTTCCAACCATAAAAACCA12530.119221945972382No Hit
CACCATCAGCGGCCTGCAGGCTGAAGATGTGGCGCTGTATTACTGTCAACAATATTATAGTTCTCCTCTCACTTT12440.1183656031840728No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12360.1176044095944646No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12280.1168432160048564No Hit
GGCTGAAGATGTGGCGCTGTATTACTGTCAACAATATTATAGTTCTCCTC12030.11446448603733084No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11960.11379844164642366No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11840.1126566512620114No Hit
GTCTGGGGCAGAGGTGAAGAACTCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTAGTGGATACACCTTCACCGG11720.11151486087759913No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC11720.11151486087759913No Hit
ATATTACTGTGCGCGGGGGGATATTGTAGTAGAAGCACCAGCCGGTGTTT11690.11122941328149605No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11690.11122941328149605No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAA11630.11065851808928992No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAAGG11620.11056336889058889No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11550.10989732449968172No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11550.10989732449968172No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11430.10875553411526945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11290.10742344533345512No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11040.10504471536592955No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10990.10456896937242444No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10970.10437867097502238No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10800.10276113459710498No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10720.1019999410074968No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10570.10057270302698146No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10560.10047755382828043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)