FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404390
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTTACCCTCGGACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT102290.7283589316357992No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCGGTTGATGGTGAGAGTGAAATCTGTC90260.6426989653871076No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45590.32462492612450955No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42110.29984548451640924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34890.24843526370879881No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29120.20734981023789686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28540.2032199033032135No Hit
GTGCAAAGTGGATGCAGCATACATCAGGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAA26660.18983330841148113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26550.18905005019973084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26370.18776835494413946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25360.18057662045443218No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25340.18043420987047756No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25250.17979336224268186No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25180.17929492519884077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24050.1712487272054059No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23940.1704654689936556No Hit
GTAATACACAGCCGTGTCTTCGACTCTGAGGCTGTTCATTATCACATAGA22720.16177842337242504No Hit
GTGGGGTCCCATCAAGATTCAGCGGCACTGGATCTGGGACAGATTTCACT22410.15957105932112875No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21780.15508512592655885No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20680.1472525438090559No Hit
GGGTATCAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCCTGATGTATGCTGCATC20510.1460420538454418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20380.1451163850497369No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20350.14490276917380499No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20040.14269540512250872No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19320.13756862410014312No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18630.13265545895370945No Hit
GGACCCCACTGTGCAAAGTGGATGCAGCATACATCAGGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG18270.13009206844252666No Hit
GATGTATGCTGCATCCACTTTGCACAGTGGGGTCCCATCAAGATTCAGCGGCACTGGATCTGGGACAGATTTCA17920.12759988322332116No Hit
GTGTATTACTGTGCGAGAGTAGTCTGTCTGGCACCACCCTGGCAGTACAA17790.12667421442761626No Hit
TCCTCACTCTCTGCATCTACAGGAGACAGAGTCACCATCACTTGTCGGGC17350.12354118158061508No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCGCTGAATCTTGATGGGACCCCA17270.12297153924479666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17170.12225948632502368No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17120.12190345986513716No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16990.1209777910694323No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16830.11983850639779549No Hit
GTAATAAGTTGCAAAATCTTCAGACTGCAGGCGGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCG16780.11948247993790898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16570.11798716880638568No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16520.1176311423464992No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16420.11691908942672619No Hit
GATTTCACTCTCACCATCAACCGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTAC16110.11471172537542991No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15780.112361950740179No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15690.11172110311238333No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTC15550.11072422902470111No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15240.10851686497340483No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15110.10759119617769994No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14860.10581106387826743No Hit
CATCAGGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT14820.10552624271035822No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14570.10374611041092574No Hit
GTCTCCATCCTCACTCTCTGCATCTACAGGAGACAGAGTCACCATCACTT14480.10310526278313004No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14070.10018584581206075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4050.046.8884541
ATCTCGT3400.046.29955337
TCGTATG4150.044.79603640
CACGAGA4800.044.29821
ACGAGAC4850.043.84287622
ATGCCGT4200.042.9747344
CTTATAC5150.042.5754171
AATCTCG3700.042.45965636
CGAGACA5200.042.19909323
TATACAC5550.041.938113
TGCCGTC4400.041.11170245
GCCGTCT4450.040.71854846
GTGGTAT3400.040.6778221
TATCGTA603.0629963E-739.9899838
AGGCAAT5100.039.3027232
GTATGCC4900.038.8169842
GTCTCGT350.001505438238.5518071
TTATACA5650.037.6135982
TCCGAGC5850.037.48092313
CGAGCCC5850.037.48092315
Produced by FastQC (version 0.11.5)