FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404390
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTTACCCTCGGACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT96400.6864190146611696No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCGGTTGATGGTGAGAGTGAAATCTGTC81670.581533619578607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46140.32854121718326107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43520.3098854306852085No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33910.24145714509502345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32380.23056273542249658No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29860.21261900184421706No Hit
GTGGGGTCCCATCAAGATTCAGCGGCACTGGATCTGGGACAGATTTCACT28590.2035759297631No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25570.18207193158595547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25420.18100385220629597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25020.17815564052720398No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24900.1773011770234764No Hit
GTGCAAAGTGGATGCAGCATACATCAGGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAA24890.17722997173149907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24760.1763043029357942No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22160.15779092702169625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22050.15700766880994593No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21200.15095521899187547No Hit
GGGTATCAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCCTGATGTATGCTGCATC20960.14924629198442027No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20760.1478221861448743No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20450.14561482209357798No Hit
GTAATACACAGCCGTGTCTTCGACTCTGAGGCTGTTCATTATCACATAGA18980.13514764417291492No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18950.13493402829698303No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18620.13258425366173213No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18390.13094653194625425No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18270.13009206844252666No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18110.12895278377088984No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18090.12881037318693525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17790.12667421442761626No Hit
GATGTATGCTGCATCCACTTTGCACAGTGGGGTCCCATCAAGATTCAGCGGCACTGGATCTGGGACAGATTTCA16790.11955368522988628No Hit
GGACCCCACTGTGCAAAGTGGATGCAGCATACATCAGGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG16590.11812957939034029No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16450.1171327053026581No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCGCTGAATCTTGATGGGACCCCA16270.11585101004706669No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16070.1144269042075207No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTC15600.11108025548458762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15580.11093784490063302No Hit
GATTTCACTCTCACCATCAACCGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTAC15350.10930012318515511No Hit
GTAATAAGTTGCAAAATCTTCAGACTGCAGGCGGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCG15150.10787601734560913No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14900.10609588504617665No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14880.10595347446222202No Hit
GTGTATTACTGTGCGAGAGTAGTCTGTCTGGCACCACCCTGGCAGTACAA14600.10395972628685765No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14600.10395972628685765No Hit
TCCTCACTCTCTGCATCTACAGGAGACAGAGTCACCATCACTTGTCGGGC14490.10317646807510734No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14390.10246441515533433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14310.10189477281951594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.034.9287381
GTATCAA33550.034.6648331
CGAGCTA400.002649861634.35719739
TATCAAC37200.031.0612932
ATCAACG37750.030.2345523
GCTTGTA1351.2732926E-1129.9647031
CCTAACG450.005194529429.9444162
AATCTGT28600.029.83669968
TCAACGC38900.029.5951124
CGAACTG28350.029.5290553
CAACGCA39300.029.2949315
GACAGTA26050.029.07904214
GAACTGT28700.028.99573554
ACGAACT28800.028.98066352
TAATACT26200.028.9084434
CGGTTGA26650.028.84683449
AATACTG26450.028.7634965
GCGGTTG26550.028.74747548
AACGCAG40550.028.7239516
TTCGGCC28650.028.70963923
Produced by FastQC (version 0.11.5)