FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106826
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCGTGGGATGACAGTTTGAGTGGTCAAACAGTATTCGGCGGAGGGACCAAGCTGACCGTC38460.34748009172173405No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATAAAGTGTATTCCTGGAATTGGCTCTGGA35690.32245357445524414No Hit
CTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTTTTGGTACCA35560.32127904476403696No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG35540.32109834788846664No Hit
CTATAGGAGTGATCAGCGGCCCCCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC27770.25089761172939556No Hit
GTATATTACTGTGCGAGATATTTGGAACTCGGGTGGACAGTGGCTGGTCA27320.2468319320290633No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24580.22207646007592882No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22460.20292259126547443No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22090.19957969906742343No Hit
GTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAACGTCTGGGGCCC20390.18422046464394584No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19240.17383039429865219No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC18600.1680480942804018No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC18530.16741565521590565No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18310.1654279895846321No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17650.15946499269081138No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTG17330.1565738426816862No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17210.15548966142826423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16780.15160467860350227No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG16250.14681621140088866No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15600.1409435629448531No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAACGTCT15250.13778136762237245No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15160.13696823168230599No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14980.13534195980217306No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14880.13443847542432144No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14870.13434812698653628No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14650.1323604613552627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14540.1313666285396259No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14310.1292886144705672No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14190.12820443321714525No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14100.1273912972770788No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13990.12639746446144198No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13890.12549398008359036No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTGT13890.12549398008359036No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13830.1249518894568794No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13820.12486154101909425No Hit
GGGTAACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGGACAAATTAT13810.12477119258130907No Hit
CCCCTGGGGGCCGCTGATCACTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTTTTGGTACCAGTATA13750.12422910195459812No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13640.12323526913896132No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13450.12151864882104324No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13250.11971168006534No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13160.11889854412527354No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13040.11781436287185158No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13030.11772401443406642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12930.11682053005621479No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12780.11546530348943737No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12520.11311624410702316No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12430.11230310816695667No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12430.11230310816695667No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTGTTTGACCACTC12410.11212241129138636No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12310.11121892691353474No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12170.10995404878454246No Hit
GTGCTGAATCAGCCACCCTCAACGTCTGGGGCCCCCGGGCAGAGGGTAAC12070.10905056440669084No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11990.10832777690440955No Hit
TTACTGTGCAGCGTGGGATGACAGTTTGAGTGGTCAAACAGTATTCGGCG11940.10787603471548374No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11850.10706289877541729No Hit
GTCAAACAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC11780.10643045971092113No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11680.10552697533306951No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11640.10516558158192887No Hit
CCCCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT11540.10426209720407725No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11500.10390070345293659No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11490.10381035501515143No Hit
CCTTTAGTAGTTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG11100.10028676594153009No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCAC11080.10010606906595979No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11070.10001572062817463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1750.050.9725138
TCGTATG1700.048.6045840
CGTATGC1800.045.96769341
CGAGACG1950.045.14502323
CTTATAC2100.045.130751
ATCTCGT2000.044.53161637
TCCTATC2050.043.1985833
GGGCGTT805.6330467E-542.26043770
CCCGACA650.001033733541.61027570
AGGGGGG2150.040.88451870
GACGGAC2100.040.34452426
TATACAC2400.039.4894033
CTATCTC2450.039.0166635
GTATCAA16000.038.5021741
GGTATCA6250.038.4513971
CCCACGA2400.038.07906719
GCCGTCT2200.037.99281746
CGGACTC2250.037.69069328
GTATGCC2300.037.52892742
TCTCCGA2450.037.3036311
Produced by FastQC (version 0.11.5)