FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854281
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27860.32612220100880157No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22830.26724227742393897No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGAGAGTGTTCCTCCTTGTGTATGAA22420.2624429198355108No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19090.22346277161730158No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17930.20988410136711455No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17190.20122184620751252No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16640.19478368358888937No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15710.18389733588830842No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15570.18225853085811342No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15220.178161518282626No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14900.17441567821360887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14570.17055278064243498No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14300.16739222808420182No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTCATTATTATTGCAGTTCATAC13990.16376344551734148No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13990.16376344551734148No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTT13610.1593152604353837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13280.1554523628642098No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACATCACTCAGGCAAGGC13160.15404767283832838No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGAGAGTGTTC13000.1521747528038198No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACATCA12900.15100417778225197No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12030.14082017509461173No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11940.13976665757520068No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11810.13824491004716247No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11740.13742550753206498No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11720.13719139252775142No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11480.13438201247598858No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11430.13379672496520467No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11380.13321143745442074No Hit
GTAGTATACGCCTGTGTCCTCGCTTTTCAGGCTGCTCATTTGCAGATACA11320.13250909244148001No Hit
GTTCATACACAAGGAGGAACACTCTCGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG11250.13168968992638255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11180.13087028741128504No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11090.12981676989187396No Hit
GGCGTATACTACTGTGTCACAAGTTCGTTGGGGGGAAACTGTCGTGAAAC10520.12314449226893728No Hit
GTACCAACATCACTCAGGCAAGGCCCCCAAACTCATGATTTATGAGGTCA10460.12244214725599656No Hit
GTGTATGAACTGCAATAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGA10400.12173980224305585No Hit
GTTATCGGCCCTCGGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC10320.12080334222580158No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10290.12045216971933123No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10210.11951570970207695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10060.11775984716972518No Hit
GAGGAACACTCTCGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT10010.11717455965894127No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9990.11694044465462769No Hit
GGTTATAACCATGTCTCCTGGTACCAACATCACTCAGGCAAGGCCCCCAAACTCATGATTTATGAGGTCAGTTAT9980.1168233871524709No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9920.11612104213953021No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9770.11436517960717843No Hit
GTATACGCCTGTGTCCTCGCTTTTCAGGCTGCTCATTTGCAGATACACTG9720.11377989209639451No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9430.11038522453384776No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9380.10979993702306384No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9130.10687349946914423No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9130.10687349946914423No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9130.10687349946914423No Hit
GGGCACAGAGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9050.10593703945188995No Hit
GGCAAGGCCCCCAAACTCATGATTTATGAGGTCAGTTATCGGCCCTCGGG8990.10523469443894924No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8930.10453234942600854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8930.10453234942600854No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGAGTCCTGGGCCCAGTCTGCCCTGACTCAGC8830.10336177440444069No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8630.10102062436130502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)