FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854281
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25060.2933461004049019No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22490.26326232235060826No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20600.24113845444297602No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20130.23563675184160715No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGAGAGTGTTCCTCCTTGTGTATGAA19950.23352971680278503No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG16960.19852952365790646No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16170.1892819809875205No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15650.18319499087536772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14840.17371333320066815No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14220.16645576806694753No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13980.1636463880151847No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTT13730.16071995046126508No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13700.16036877795479473No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13460.1575593979030319No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTCATTATTATTGCAGTTCATAC13340.1561547078771505No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13180.15428178784264193No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACATCACTCAGGCAAGGC12950.15158946529303588No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACATCA12150.14222486512049315No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11670.1366061050169675No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11660.1364890475148107No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11560.13531847249324286No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11400.1334455524587343No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11290.13215791993500967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10720.12548564231207296No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10640.12454918229481869No Hit
GGTTATAACCATGTCTCCTGGTACCAACATCACTCAGGCAAGGCCCCCAAACTCATGATTTATGAGGTCAGTTAT10610.12419800978834834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10280.12033511221717444No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10130.11857924968482268No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGAGAGTGTTC10100.1182280771783523No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10010.11717455965894127No Hit
GGCGTATACTACTGTGTCACAAGTTCGTTGGGGGGAAACTGTCGTGAAAC9890.11576986963305984No Hit
GGGCACAGAGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9850.11530163962443271No Hit
GTGTATGAACTGCAATAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGA9820.11495046711796236No Hit
GTAGTATACGCCTGTGTCCTCGCTTTTCAGGCTGCTCATTTGCAGATACA9770.11436517960717843No Hit
GTACCAACATCACTCAGGCAAGGCCCCCAAACTCATGATTTATGAGGTCA9740.11401400710070808No Hit
GAGGAACACTCTCGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT9730.11389694959855129No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9690.11342871958992416No Hit
GTTATCGGCCCTCGGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC9640.11284343207914023No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9420.11026816703169097No Hit
GTTCATACACAAGGAGGAACACTCTCGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9390.10991699452522062No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9310.10898053450796635No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9200.10769290198424171No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9170.10734172947777136No Hit
GTATACGCCTGTGTCCTCGCTTTTCAGGCTGCTCATTTGCAGATACACTG9010.10546880944326281No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9000.10535175194110603No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9000.10535175194110603No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGAGTCCTGGGCCCAGTCTGCCCTGACTCAGC8700.10184002687640249No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8650.10125473936561857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA200.002865743865.66759569
TACGTAT350.003914295760.6936170
GTACGTA301.9473652E-458.37119369
GCAACGG200.00671636352.95850856
ACCCGTT458.750867E-751.32026768
GGTATCA9250.041.6183431
GTATCAA22550.038.3738371
GTGGTAT2850.035.861171
TGATAGC400.002615319834.44772338
CGAACTA400.00276930534.04815724
CTCGCTA400.002770100534.0461623
TGGTATC3300.033.0125242
TATCAAC26350.032.945972
TCAACGC26550.032.296084
ATCAACG26950.031.94863
CAACGCA27050.031.699115
TTAGGAC553.527683E-430.938373
AACGCAG28250.030.35266
CTAGACA450.00494543430.2455374
TATGGTA450.00494543430.2455374
Produced by FastQC (version 0.11.5)