FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148410
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGA68620.5975217909979885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54490.47448211004780516No Hit
CAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA52980.4613334958769081No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49710.43285934465913745No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA39770.34630489111031776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36120.31452181712106303No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35440.30860058689840736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35070.3053787410419624No Hit
GTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT31640.27551135918356684No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31130.2710704365165751No Hit
GTCTGGGACAGACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACT30070.2618402835224354No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29840.25983751447653713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29700.2586184376659904No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28000.24381536210935117No Hit
GTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATG27700.24120305465817957No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26040.22674828676169662No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24870.2165602877021273No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24790.21586367238181484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24740.21542828780661957No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACTTTGGTCCCCCGGCCAAAAGTC24700.2150799801464634No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACT24330.21185813429001835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24220.21090028822458878No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCT24190.21063905747947162No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23500.2046307503417769No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23230.2022796736357224No Hit
CTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCA22990.20018982767478513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22650.19722921256345732No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGC21530.18747659807908323No Hit
ATGTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAA20980.18268736775193528No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20570.17911721423533405No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20170.1756341376337719No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCA19540.1701482919863115No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGATACCAGGCCAAGTAACCGCTC19530.17006121507127248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACTTTGGTCC19410.1690162920908038No Hit
GTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA19310.16814552294041327No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC18550.16152767739744517No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18320.15952490835154692No Hit
GTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18170.15821875462596113No Hit
GACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACTTTGCAATGTATTACTGTCAGCAATATGGTGCCTCA17920.15604183174998476No Hit
GAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC17570.1529941397236179No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17490.15229752440330543No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCACCTGGAGACAAA17470.15212337057322733No Hit
GTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACC17200.1497722938671729No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.14794367865135274No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16880.14698583258592315No Hit
CCATATTGCTGACAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACT16720.14559260194529827No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16610.1446347558798687No Hit
GCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAACTGTGGCTGCACCAT16580.14437352513475152No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16470.14341567906932193No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTC16140.14054214087303316No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16110.140280910127916No Hit
GCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCAGACAGGTT15380.13392429533006506No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15060.13113783404881532No Hit
TCTATGATGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGAGGGTCTGGGACAGACTTCACT15010.13070244947362006No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14910.1298316803232295No Hit
CACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGAGGGTCTGGGACAGACTTCACTCTCATCGTCAGTCG14860.12939629574803424No Hit
ATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAACTGTGGCTGCACCAT14800.1288738342577999No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTCTC14780.1286996804277218No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14770.12861260351268275No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14180.12347506552537857No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14080.12260429637498804No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13860.12068860424412885No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13830.12042737349901168No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13590.11833752753807437No Hit
CAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGA13560.11807629679295721No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13530.11781506604784005No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13200.11494152785155128No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13070.11380952795604357No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13050.11363537412596547No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13010.11328706646580924No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12920.11250337423045777No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12660.11023937443944236No Hit
ATATTGCTGACAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGA12580.10954275911912992No Hit
GTAATATATGGCGCTGTCCGAGGCGCTCACGTAGTTCCACTGCAGGTAGG12520.10902029762889562No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12500.1088461437988175No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12440.10832368230858316No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12380.10780122081834884No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12350.1075399900732317No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12240.1065821440078021No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12050.10492768262206006No Hit
GGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCA11930.10388275964159142No Hit
CTGCAGGAGAGGGTGGCTCCTTCACCTGGAGACAAAGACAGGGTGCCTGG11920.10379568272655237No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11740.10222829825584939No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11590.10092214453026357No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTA11560.10066091378514642No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11500.1001384522949121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)