FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148410
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGA65320.5687864090351007No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53790.4683867259950714No Hit
CAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA46700.4066491932323822No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41690.3630236587978161No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA37910.3301085849130537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35710.3109516636044618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35350.3078168946630559No Hit
GTCTGGGACAGACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACT33620.2927525883612996No Hit
GTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT31240.2720282825820047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31180.27150582109177035No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31150.2712445903466532No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28510.24825628477634296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28010.24390243902439024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27240.23719751656638308No Hit
GTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATG26880.23406274762497714No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24970.21743105685251785No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24870.2165602877021273No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACTTTGGTCCCCCGGCCAAAAGTC24800.2159507492968539No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24630.21447044174119No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24490.21325136493064323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24440.21281598035544796No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACT24380.21229351886521364No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCT24140.21020367290427636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22940.19975444309958987No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22920.19958028926951177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22510.19601013575291054No Hit
CTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCA21900.19069844393552823No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGC21810.18991475170017677No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21270.18521259828806785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20350.17720152210447487No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20320.17694029135935774No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20270.17650490678416245No Hit
ATGTATTACTGTCAGCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAA20010.17424090699314704No Hit
GTCTGGGAGCCTGGCCAGGCTTCTGCTGATACCAGGCCAAGTAACCGCTC19700.17154152262693637No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19380.16875506134568663No Hit
GTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACCCTCTGCCA18320.15952490835154692No Hit
GTCTTTGTCTCCAGGTGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18240.1588282930312345No Hit
GAGTGTGAGCGGTTACTTGGCCTGGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC18160.15813167771092207No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC18050.15717383164549245No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGCCACTGAACCTGTCTGGGATGCCA17990.15665137015525815No Hit
GACTTCACTCTCATCGTCAGTCGCCTGGAGCCTGAAGACTTTGCAATGTATTACTGTCAGCAATATGGTGCCTCA17840.15534521642967233No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACTTTGGTCC17650.1536907550439303No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17580.15308121663865693No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17090.14881444780174327No Hit
GTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGACC16760.1459409096054545No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16290.14184829459861895No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15910.1385393718271349No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15860.13810398725193962No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTC15760.1372332181015491No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACGCAGTCTC15630.1361012182060414No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15480.13479506448045558No Hit
GCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCAGACAGGTT15440.13444675682029938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15350.1336630645849479No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15290.13314060309471354No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCACCTGGAGACAAA15240.1327052185195183No Hit
CCATATTGCTGACAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACT15200.13235691085936208No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15110.13157321862401058No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14830.12913506500291708No Hit
TCTATGATGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGAGGGTCTGGGACAGACTTCACT14610.1272193728720579No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14540.1266098344667845No Hit
GCAATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAACTGTGGCTGCACCAT14190.12356214244041762No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14100.12277845020506614No Hit
ATATGGTGCCTCACCAGGGACTTTTGGCCGGGGGACCAAAGTCGAGATCAAACGAACTGTGGCTGCACCAT13790.12007906583885546No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13550.11798921987791816No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13550.11798921987791816No Hit
GGTATCAGCAGAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCA13490.11746675838768385No Hit
CACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGAGGGTCTGGGACAGACTTCACTCTCATCGTCAGTCG13240.11528983551170749No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13100.11407075870116075No Hit
GTAATATATGGCGCTGTCCGAGGCGCTCACGTAGTTCCACTGCAGGTAGG12630.1099781436943252No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12620.10989106677928615No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12620.10989106677928615No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12160.10588552868748966No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12100.10536306719725534No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12060.10501475953709911No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11820.10292491357616182No Hit
ATATTGCTGACAGTAATACATTGCAAAGTCTTCAGGCTCCAGGCGACTGACGATGAGAGTGAAGTCTGTCCCAGA11760.1024024520859275No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGCGGTTACTTGGCCTGGTA11730.10214122134081034No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11720.10205414442577129No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11600.10100922144530262No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11540.10048675995506832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA405.7466124E-542.122481
GTATCAA19600.037.4804081
CGCGCTA601.5403579E-533.8143128
AACTGTG24650.033.42383270
AATGGTA6050.033.1870670
TATCAAC22300.032.483432
TCAACGC22450.032.1163144
ATCAACG22700.031.9110343
CAACGCA22700.031.7653775
AACGCAG22850.031.711226
CGGCTGA5050.030.20999169
ACGCAGA24050.029.9927377
TCTAGAC450.00519063829.9485473
AGAGTAC24150.029.89458711
CGCAGAG24350.029.630968
TGTTAGA13150.029.4841470
GTGGTAT2650.029.2473091
GCAGAGT25050.028.807979
GATTCAC1850.027.3226871
GTCGTAC500.00867536726.9583851
Produced by FastQC (version 0.11.5)