FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339891
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51920.38749420661829953No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50420.3762992661343348No Hit
GATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAG48090.3589097919159096No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGACCTCCCATGCTCCACAGTAATAATCAG39630.2957703275863484No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39330.29353133948955545No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC38950.2906952879002844No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38100.2843514882927044No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGACG37470.2796496132894392No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35340.2637527978022093No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT30180.22524220253737057No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29250.21830133943731245No Hit
CCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC29020.21658478189643784No Hit
CTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATA28070.20949465292326017No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27490.2051659426027938No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26840.2003148017264091No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26750.19964310529737123No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26720.1994192064876919No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGA25800.19255297632419355No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25370.18934376005212364No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24990.18650770846285256No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC24860.18553748028757563No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24820.18523894854133657No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24480.18270142869830458No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG24250.18098487115742998No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22690.16934213305410664No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22400.16717777789387347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22120.16508805567020005No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22020.1643417263046024No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21460.16016228185725553No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA21110.15755012907766378No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20520.15314678582063765No Hit
CCCCTGAGGGCCGCTCACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATACCAGTAAA20150.15038536716792636No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19950.14889270843673105No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19800.14777321438833457No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18880.1409069842248362No Hit
CCTGAGGGCCGCTCACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCC18270.13635437509469053No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAAT18060.13478708342693546No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17450.1302344742967898No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17100.12762232151719805No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16950.12650282746880157No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16850.1257564981032039No Hit
CACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGA16620.12403994056232934No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGCTCATTTGCAGGAACA16550.12351751000641095No Hit
ATAGGAATAGTGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCGTCCCTGA16450.12277118064081333No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16440.12269654770425355No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16350.12202485127521566No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16170.1206814584171399No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16070.11993512905154224No Hit
GATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATACCAGTAAACAT16020.11956196436874343No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15910.11874100206658601No Hit
GTATACGGTACTAGTACTAGGACTAATATATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT15800.11792003976442861No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15790.11784540682786883No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15710.11724834333539072No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15710.11724834333539072No Hit
ACCGTATACTATGCAGACTCTGTCAAGGGCCGATTCACCGTCTCCAGAGACAATGCCGACAACTCACTGTTCCTG15680.11702444452571141No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15640.11672591277947235No Hit
GTCTGGCACCTCAGCGTCCCTGACCATCAGTGGGCTCCGGTCCGAGGATG15410.11500935523859777No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15330.11441229174611964No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15040.11224793658588647No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA15000.11194940483964741No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14790.11038211317189234No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14610.10903872031381658No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGT14480.10806849213853963No Hit
GTGTATTACTGCACCGTGGAACCGACTGCTATATCGCACTCTGATTCCTGGGGCCAGGGAACCCTGGTCACCGT14450.10784459332886032No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14420.10762069451918103No Hit
CATAAGAGGTGACCTCCCATGCTCCACAGTAATAATCAGCCTCATCCTCG14390.10739679570950175No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCGTCCCTGACCATCAGTGGGCTCCGGT14310.10679973221702363No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14260.1064265675342248No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14070.10500854173958926No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC14010.10456074412023067No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13690.1021724901503182No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13430.10023203379976431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)