FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339891
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45140.3368930756307789No Hit
GATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAG44280.33047464308663915No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC41630.3106969148983014No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41620.31062228196174163No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA39180.292411845441159No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35320.26360353192908975No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33680.2513637303332883No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32410.24188534739019818No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31540.2353922819094986No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGACCTCCCATGCTCCACAGTAATAATCAG29680.2215105557093823No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26920.2009118652188872No Hit
CCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC26890.2006879664092079No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25950.19367247037259003No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25820.19270224219731308No Hit
CTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATA25420.18971692473492247No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC25410.1896422917983627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25280.18867206362308575No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG25190.1880003671940479No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24590.18352239100046197No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23910.17844735131439796No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23780.177477123139121No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22970.17143185527778007No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22590.168595803688509No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGGTGA22510.16799874019603087No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22020.1643417263046024No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21640.16150567471533134No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21160.1579232937604626No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20420.15240045645504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19930.14874344256361152No Hit
ATAGGAATAGTGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCGTCCCTGA19640.14657908740337833No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19140.1428474405753901No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19110.1426235417657108No Hit
CCCCTGAGGGCCGCTCACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATACCAGTAAA19050.1421757441463522No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18480.1379216667624456No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAAT18300.13657827390436983No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17230.128592549692475No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA16750.1250101687376063No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16680.1244877381816879No Hit
GTCTGGCACCTCAGCGTCCCTGACCATCAGTGGGCTCCGGTCCGAGGATG16590.12381604175265003No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16530.12336824413329145No Hit
CCTGAGGGCCGCTCACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCC16120.12030829373434108No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16110.1202336607977813No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16000.1194126984956239No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15820.11806930563754814No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15540.11597958341387471No Hit
GTGTATTACTGCACCGTGGAACCGACTGCTATATCGCACTCTGATTCCTGGGGCCAGGGAACCCTGGTCACCGT15510.11575568460419541No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15500.11568105166763565No Hit
CACTATTCCTATAGATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGA15000.11194940483964741No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14940.11150160722028882No Hit
GTATACGGTACTAGTACTAGGACTAATATATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT14760.11015821436221306No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCGTCCCTGACCATCAGTGGGCTCCGGT14720.10985968261597398No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14680.10956115086973492No Hit
GATGAGGACTTTGGGGGCCGTTCCTGGGACTTGCTGATACCAGTAAACAT14650.10933725206005564No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGCTCATTTGCAGGAACA14640.10926261912349586No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14560.10866555563101776No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14530.10844165682133845No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGGAGCATGGGAGGTCACCTCTTATGTCTTCGGAACTGGGACCAAGGT14510.10829239094821892No Hit
GGAATAGTGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG14420.10762069451918103No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14300.10672509928046386No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14190.10590413697830643No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14170.10575487110518693No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC13860.10344125007183419No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13800.1029934524524756No Hit
ACCGTATACTATGCAGACTCTGTCAAGGGCCGATTCACCGTCTCCAGAGACAATGCCGACAACTCACTGTTCCTG13790.10291881951591585No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13780.1028441865793561No Hit
CCATTTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAATCATGTTTACTGGTATCAGCAAGTCCCAGGAACGG13730.10247102189655725No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13610.10157542665784008No Hit
GTACTAGTACTAGGACTAATATATGAAATCCACTCCAGCCCCTTCCCTGG13400.10000813499008501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT100.0067322445147.5344170
GCTTCGT200.007867780550.8827716
TTTCGAT450.00899969249.17814370
CAACGAA306.866217E-445.2206885
GTGGTAT3700.041.288671
TATCAAC32650.032.840752
GTATCAA35000.032.7845151
ATCAACG33700.031.7049963
GACAGGT2300.030.9964221
GCACGGT450.00439144130.99036248
TCAACGC34400.030.6619934
CAACGCA35150.030.1042425
AACGCAG35400.029.9874466
ACGCAGA37800.027.9040347
CGCAGAG38250.027.4870858
TCCTTAC500.008387946527.1455842
AACGAAG500.00840782827.132416
AGAGTAC39050.027.00678811
ACTCGTA1605.0931703E-1126.76110659
GCAGAGT39650.026.687629
Produced by FastQC (version 0.11.5)