FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176927
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64560.5485471911172061No Hit
GTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATCGCCTGGGTCTCCCTGATGGTCAGAGTGG49230.41829272333798106No Hit
GCCTTGGGCTGACCGAGGACGGTCATCTTGGTCCCACCGCCGAAGACGACCCGTGCAGTATTACTGTCCCACGC46760.3973058651895997No Hit
CACCTGTAGTGGTCATTTATCAAGATGAAAAGCGGCCCTCAGGGATCTCT45420.3859202822265102No Hit
CAGTAATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCC38970.3311165433370124No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33180.28192062889202135No Hit
CTACAGGTGACTGGCCTGGCTTCTGCTGATACCACCAAACATATCTATTC30960.26305794666958954No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28810.24479003370642358No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27940.23739790148411927No Hit
GTAATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCCTC24790.21063328481715518No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCATCTTGGTCCCAC24050.2043457240763446No Hit
CAGTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATCGCCTGGGTCTCCCTGATGGTCAGAGTGG23840.202561416298547No Hit
CCCTCAGTGTCTGTGTCCCCAGGACAGACAGCCAGTATCACCTGCTCTGG23720.2015418118540912No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23630.20077710852074937No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21750.18480330555760893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21140.17962031629829206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20690.17579679963158293No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19390.16475108481664538No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19260.16364651333515165No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19250.16356154629811365No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19010.1615223374092021No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18830.15999293074251844No Hit
GTATTATTGTGCGAGACTCTCATCCGACGGAATAGGAGTTATAAATGATG18440.1566792162980372No Hit
ATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA18360.15599948000173333No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18330.15574457889061938No Hit
CAGCCACTCTGACCATCAGGGAGACCCAGGCGATGGATGAGGCTGATTATTTCTGTCAGGCGTGGGACAGTAATA18040.15328053481651793No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17890.15200602926094822No Hit
CCCCAGGACAGACAGCCAGTATCACCTGCTCTGGAGATCAATTGGGGAAT17880.15192106222391022No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17570.14928708407573282No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17540.14903218296461887No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17340.14733284222385926No Hit
GTATCACCTGCTCTGGAGATCAATTGGGGAATAGATATGTTTGGTGGTATCAGCAGAAGCCAGGCCAGTCAC17280.14682304000163138No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16720.1420648859275044No Hit
CAATAATACATGGCCGCGTCCGCAGCGGTCACAGAATTTACGTTCAAAGA16690.14180998481639048No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16510.14028057814970682No Hit
CATATGACTTGACTCAGTCACCCTCAGTGTCTGTGTCCCCAGGACAGACA16210.1377315670385674No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16160.1373067318533775No Hit
GTGCAGTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATC15670.1331433470385164No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15480.13152897333479477No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15420.1310191711125669No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15270.12974466555699715No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGAGATCCCT15230.12940479740884525No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14960.12711068740881976No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14650.12447670926064233No Hit
GAATAGGAGTTATAAATGATGATGGTCTTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCC14290.12141789592727502No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14250.12107802777912308No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14140.12014339037170531No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14120.11997345629762934No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13900.11810418148279374No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13830.11750941222352787No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13790.11716954407537596No Hit
GGATCCGTGGCCTCATATGACTTGACTCAGTCACCCTCAGTGTCTGTGTCCCCAGGACAGACAGCCAGTATCAC13770.1169996100013No Hit
GCACAGGATCCGTGGCCTCATATGACTTGACTCAGTCACCCTCAGTGTCT13600.11555517037165433No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG13550.11513033518646441No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13510.11479046703831249No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13480.11453556592719853No Hit
ACCAAGTACAGCCCCTCCCTCAAGGGTCGAGTCTCCATCTCATTAGACGC13460.11436563185312258No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13300.11300615926051488No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12940.10994734592714756No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12860.10926760963084373No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12850.10918264259380574No Hit
CTCCTATTCCGTCGGATGAGAGTCTCGCACAATAATACATGGCCGCGTCC12850.10918264259380574No Hit
GTACAGCCCCTCCCTCAAGGGTCGAGTCTCCATCTCATTAGACGCGTCCA12840.10909767555676775No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12630.10731336777897016No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12340.1048493237048687No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12310.10459442259375476No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCATATGACTTGACT12290.1044244885196788No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12070.10255521370484322No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11910.1011957411122355No Hit
GTCCTGGACGTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11800.10026110370481771No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11780.10009116963074174No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCATC11770.10000620259370378No Hit
GTCACCCTCAGTGTCTGTGTCCCCAGGACAGACAGCCAGTATCACCTGCT11770.10000620259370378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)