FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176927
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58380.49603756222773376No Hit
GTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATCGCCTGGGTCTCCCTGATGGTCAGAGTGG44850.3810771611153453No Hit
GCCTTGGGCTGACCGAGGACGGTCATCTTGGTCCCACCGCCGAAGACGACCCGTGCAGTATTACTGTCCCACGC44740.3801425237079275No Hit
CACCTGTAGTGGTCATTTATCAAGATGAAAAGCGGCCCTCAGGGATCTCT42830.36391381963367314No Hit
CAGTAATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCC36330.30868524555898535No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31010.2634827818547794No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29540.2509926274101962No Hit
CTACAGGTGACTGGCCTGGCTTCTGCTGATACCACCAAACATATCTATTC28280.24028678074341062No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27920.23722796741004326No Hit
GTAATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCCTC25150.21369209815052254No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23410.1989078337059138No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22880.1944045807429008No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCATCTTGGTCCCAC21660.1840386022242671No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21600.18352880000203922No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21320.18114972296497572No Hit
CAGTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATCGCCTGGGTCTCCCTGATGGTCAGAGTGG21220.18030005259459592No Hit
CCCTCAGTGTCTGTGTCCCCAGGACAGACAGCCAGTATCACCTGCTCTGG21030.17868567889087428No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20830.17698633815011466No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19050.16186220555735403No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18850.1601628648165944No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18710.15897332629806268No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18240.15497987555727755No Hit
GTATTATTGTGCGAGACTCTCATCCGACGGAATAGGAGTTATAAATGATG17520.14886224889054292No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17420.1480125785201631No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17360.1475027762979352No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17330.14724787518682128No Hit
GTATCACCTGCTCTGGAGATCAATTGGGGAATAGATATGTTTGGTGGTATCAGCAGAAGCCAGGCCAGTCAC17140.14563350148309961No Hit
ATACTGCACGGGTCGTCTTCGGCGGTGGGACCAAGATGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA16640.14138514963120058No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16640.14138514963120058No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16630.1413001825941626No Hit
CAGCCACTCTGACCATCAGGGAGACCCAGGCGATGGATGAGGCTGATTATTTCTGTCAGGCGTGGGACAGTAATA16570.1407903803719347No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16310.1385812374089472No Hit
CCCCAGGACAGACAGCCAGTATCACCTGCTCTGGAGATCAATTGGGGAAT16200.1376466000015294No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16070.13654202852003566No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15920.13526752296446595No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15560.1322087096310986No Hit
CAATAATACATGGCCGCGTCCGCAGCGGTCACAGAATTTACGTTCAAAGA15520.1318688414829467No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGAGATCCCT15210.12923486333476927No Hit
CATATGACTTGACTCAGTCACCCTCAGTGTCTGTGTCCCCAGGACAGACA15200.1291498962977313No Hit
GTGCAGTATTACTGTCCCACGCCTGACAGAAATAATCAGCCTCATCCATC14590.12396690703841445No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14530.12345710481618656No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.12337213777914857No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14070.11954862111243944No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14060.11946365407540145No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13960.11861398370502163No Hit
GGATCCGTGGCCTCATATGACTTGACTCAGTCACCCTCAGTGTCTGTGTCCCCAGGACAGACAGCCAGTATCAC13910.11818914851983173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13410.11394079666793266No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13370.11360092851978075No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13250.11258132407532498No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13220.11232642296421104No Hit
GAATAGGAGTTATAAATGATGATGGTCTTCATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCC13210.11224145592717305No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13210.11224145592717305No Hit
GCACAGGATCCGTGGCCTCATATGACTTGACTCAGTCACCCTCAGTGTCT13110.11139178555679323No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13090.11122185148271728No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12960.11011728000122352No Hit
CTCCTATTCCGTCGGATGAGAGTCTCGCACAATAATACATGGCCGCGTCC12900.10960747777899564No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12820.10892774148269178No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12800.10875780740861583No Hit
ACCAAGTACAGCCCCTCCCTCAAGGGTCGAGTCTCCATCTCATTAGACGC12670.10765323592712207No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12660.1075682688900841No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12560.10671859851970429No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG12470.10595389518636245No Hit
GTCCTGGACGTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12240.10399965333448889No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12160.10331991703818504No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12060.10247024666780523No Hit
GTACAGCCCCTCCCTCAAGGGTCGAGTCTCCATCTCATTAGACGCGTCCA12040.10230031259372926No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11910.1011957411122355No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCATATGACTTGACT11850.10068593889000761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG200.00759882451.3320585
TACGGTA200.007600100251.3298762
AATGGTA5350.040.11213370
GTATCAA25650.039.0977251
ACGTTTC400.002320289435.2997959
AGGTGAT13200.034.97840570
TATCAAC29450.033.9294242
ATCAACG30800.032.440883
ACTCTGG19950.032.27066870
TCAACGC31150.032.0763784
CAACGCA31550.031.6723985
AACGCAG32300.030.838886
TCTAATG8900.029.22709370
ACGCAGA34600.028.7937957
TCTATAC605.6885596E-428.5153873
CGCAGAG34950.028.4123178
AGTACTG854.716665E-628.1823065
CCAAGGG8700.027.65655370
AGAGTAC35700.027.62946311
TAGGCCG500.00804716327.3770985
Produced by FastQC (version 0.11.5)