FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593897
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAAACTCTGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAA72200.45297782730000746No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71440.4482096396442179No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68400.42913688902105973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53970.3386040628723186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48970.30723440724212414No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT45960.28834987455274713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45090.28289155447309333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43310.2717239570687441No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43170.27084560671109864No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42150.26444619696253896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39920.25045533055147223No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39910.2503925912402119No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA39760.24945150157130602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39570.24825945465735866No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38160.23941321176964384No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG34910.21902293561001746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33380.20942382098717796No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33320.20904738511961563No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33170.20810629545070983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32610.20459289402012804No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA32590.20446741539760727No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32120.20151866776836896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32010.2008285353445047No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31870.19995018498685924No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT31740.1991345739404742No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTAGGTT31330.19656226217879824No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCGAAAGTG30310.1901628524302386No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29600.185708361330751No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC28690.1799990840060556No Hit
CTACAAACTCTGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAACG28500.1788070370921082No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT28420.1783051226020251No Hit
GTGTATTACTGTGTGAAGGTCATCGGGCAGCTACGGTTACGGTTCTACTA27570.17297228114489205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC26410.16569452103868693No Hit
CTCTGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC26150.16406329894591684No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25070.15728745332979482No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24930.1564091029721494No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT24910.1562836243496286No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24750.1552797953694624No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA24440.15333487672039034No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC24290.1523937870514845No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG23860.14969599666728778No Hit
GTGTACAGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTC23780.14919408217720467No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23710.14875490699838195No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA23590.1480020352632573No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTAGGTTCTAATCGGGTC23550.14775107801821574No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23380.14668450972678912No Hit
GTCAGGGACCCCGGGGACCCGATTAGAACCTAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA23350.14649629179300797No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23220.1456806807466229No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23200.1455552021241021No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCTGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAACGAA22870.14348480485250928No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG22640.14204180069352035No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG22620.14191632207099955No Hit
ATTTAGGTTCTAATCGGGTCCCCGGGGTCCCTGACAGGTTCAGTGGCAGT22540.14141440758091645No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22420.1406615358457918No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22090.13859113857419897No Hit
CTTTCGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22010.13808922408411586No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21780.1366462199251269No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21710.13620704474630418No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21450.13457582265353407No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21440.1345130833422737No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21360.13401116885219055No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21170.1328191219382432No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20910.13118789984547308No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20540.12886654532883868No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20200.12673340874598546No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20150.1264197121896835No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20080.1259805370108608No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19940.12510218665321535No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC19860.12460027216313226No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA19620.12309452869288293No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19600.12296905007036214No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19550.1226553535140602No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19290.12102413142129008No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19240.12071043486498814No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19170.12027125968616542No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19140.12008304175238425No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTCTGC19120.11995756312986347No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18680.11719703343440635No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18640.1169460761893648No Hit
TTATTACTGCATGCAAGGTCTACAAACTCTGTACACTTTCGGCCAGGGGA18610.11675785825558363No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18530.11625594376550054No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18340.11506389685155315No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18330.11500115754029276No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG18250.11449924305020964No Hit
GTATTACTGTGTGAAGGTCATCGGGCAGCTACGGTTACGGTTCTACTACT18050.11324445682500187No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18040.11318171751374148No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT18000.11293076026869993No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17930.1124915850898772No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17920.11242884577861681No Hit
ATCCATTACTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC17880.11217788853357526No Hit
GAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGT17570.11023296988450319No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17530.10998201263946164No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACTCTGTACACTTTCGGCCAGGGGA17370.10897818365929542No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17290.10847626916921232No Hit
GTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG16990.10659408983140066No Hit
GTATATGGTACTACTACCACTACTGATGTATGAAATCCACTCCAGCCCCTTCCCTGGAGACTGGCGGACCCAGTT16970.10646861120887988No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16840.10565300016249482No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16450.10320616702333965No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16310.1023278166656942No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA16030.10057111595040331No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTAGGTTCTAAT15950.10006920146032022No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15940.10000646214905982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAT150.001851480873.3196463
GGTATCA10200.045.6582071
CGTATGC5450.043.7233941
GTATGCC5550.043.0226142
TCGTATG5550.042.85714740
CTTATAC5400.042.496691
ATGCCGT5800.041.32908644
GCCGTCT5850.041.1659946
ACGAGAC5700.040.8623422
TCTGGAG12450.040.60724670
ATCTCGT5400.040.56067337
GTATCAA27800.040.424011
CCCACGA6000.039.93564219
CGAGACT5900.039.4846123
AATGGTA5700.039.025770
GACTAGG5900.038.9637426
CCGTCTT6200.038.93432647
TATGCCG6150.038.9091643
TTATACA5900.038.895282
CACGAGA6000.038.81800521
Produced by FastQC (version 0.11.5)