FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990418
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTTCGGCCCTGGGA51370.5186698949332504No Hit
CAGTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACCCACTGCCA42470.42880884636587785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38570.3894315329487146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33940.34268359419962074No Hit
CATCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGAGACAGATTTCA33780.34106811467481407No Hit
CTCCTGTAGGGCCAGTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACC31890.3219852627880349No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCCA30870.31168658081739226No Hit
GTGTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTTCGGCCCTGGGACCACAGTGGCTATGAAACGAA30210.3050227277775646No Hit
GTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACCCACTGCCA28150.28422342889567836No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27280.27543925897954197No Hit
CTATAGATGAGGAACCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGGAGTTTCTGCTAAGACTCTGA26240.2649386420682984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25390.25635640709276286No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24280.2451490178894164No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20170.2036513875959443No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19900.20092526589783302No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19850.20042042854633096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19450.1963817297343142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19390.19577592491251167No Hit
GTGCAGCCACAGTTCGTTTCATAGCCACTGTGGTCCCAGGGCCGAAACTG18260.1843666007685644No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT18240.18416466582796354No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18200.18376079594676187No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17980.18153951160015266No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17840.1801259670159468No Hit
GTATGCTATCTCACCATTCAGTTTCGGCCCTGGGACCACAGTGGCTATGAAACGAACTGTGGCTGCACCAT17570.1773998453178355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17160.17326017903551832No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16950.17113986215920957No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATAGCCACTGTGGTCC16770.169322447693802No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16560.16720213081749322No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16550.1671011633471928No Hit
GATAGCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAAT16190.16346633441637773No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG15630.1578121560795543No Hit
GTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACC15610.15761022113895345No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTC14930.15074443315852498No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14930.15074443315852498No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14860.15003766086642203No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14600.14741250663861116No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA14470.1460999295247057No Hit
GTCTGAGACAGATTTCACTCTCACCATCACCAGACTGGAACCTGAAGACT14220.14357574276719526No Hit
TCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGAGACAGATTTCACT13980.14115252347998522No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13250.13378189814805466No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13080.13206545115294754No Hit
CCTGTAGGGCCAGTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACCAG13010.13135867886084462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12800.1292383619845358No Hit
CTGCTACTCTGGCTCCCAGGTTCCACCGGAGAAGTTGTATTGACGCAGTC12790.12913739451423542No Hit
GTCTCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCT12540.12661320775672494No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCCACCGGAGAAGTTGTATTGACGCAGTCTC12400.12519966317251907No Hit
CCTGAAGACTTTGCAGTGTATTACTGTCAGCAGTATGCTATCTCACCATT12320.12439192341011573No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12310.12429095593981532No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12020.12136289930110317No Hit
CACCATCACCAGACTGGAACCTGAAGACTTTGCAGTGTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTT12000.12116096436050235No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11950.12065612700900023No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11440.11550678602367889No Hit
CTGTGGTCCCAGGGCCGAAACTGAATGGTGAGATAGCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGTT11430.11540581855337846No Hit
GTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACCAGCAGAAACCTGGC11350.11459807879097514No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11220.11328550167706969No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11100.11207389203346466No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11060.11167002215226299No Hit
CAATAATAGACGGCCGTGTCGTCAGATTTCAGGCTCCTCAGCTCCATATA11060.11167002215226299No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11050.11156905468196257No Hit
CTATTATTGTGCCCGTGATTTCTTTTCGTCTAGTAGTGCATCACGTTACTGGGGCCAGGGCACCCTGGTCACCGT11030.11136711974136172No Hit
GCAGAAACCTGGCCAGCCTCCCAGGTTCCTCATCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTT10870.10975164021655502No Hit
CTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGTTCCTCATCTATAGTGCATCCAGCAGGGCCC10800.1090448679244521No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10780.10884293298385125No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10760.10864099804325042No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10470.10571294140453828No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10380.10480423417183451No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10260.10359262452822948No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10100.10197714500342281No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10040.10137134018162028No Hit
ACCCTCTCCTGTAGGGCCAGTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCT9970.10066456788951736No Hit
GTCTGGGATGCCAGGGGCCCTGCTGGATGCACTATAGATGAGGAACCTGGGAGGCTGGCCAGGTTTCTGCTGGTA9970.10066456788951736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT407.87404E-570.0891170
TACTTCG200.00704772652.3181246
CGTATGC1250.046.8076141
CTTATAC2400.046.0622251
GTACGTA406.519315E-445.69622469
ACGAGAC2700.043.4388222
TCGTATG1300.042.241540
CGAGACT2850.041.15880623
TATACAC2850.041.1401023
GTATGCC1550.040.099642
TTATACA2900.039.2756232
CGCGTAT350.00139953139.12709839
GAGACTC3050.038.47736424
GTGGTAT1850.038.0267941
CACGAGA3150.037.2295221
CCCACGA3150.037.2238819
ATCTCGT1650.037.14159837
GGTATCA5750.036.7041241
TACACAT3200.036.6404045
GTGTATA656.862192E-736.0767061
Produced by FastQC (version 0.11.5)