FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990418
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTTCGGCCCTGGGA48350.48817771890252404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37970.38337348473068944No Hit
CAGTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACCCACTGCCA37400.3776183389235656No Hit
CTCCTGTAGGGCCAGTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACC33030.3334955544022827No Hit
CATCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGAGACAGATTTCA30880.3117875482876927No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30810.31108077599558975No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCCA30480.30774884947567593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28080.2835166566035755No Hit
GTGTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTTCGGCCCTGGGACCACAGTGGCTATGAAACGAA27340.27604506380134447No Hit
GTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACCCACTGCCA26070.2632221950731913No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25940.26190961795928586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25760.26009220349387835No Hit
CTATAGATGAGGAACCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGGAGTTTCTGCTAAGACTCTGA23740.2396967744931938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23600.23828322990898793No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22030.22243133707182222No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20070.20264171289294014No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19780.19971365625422802No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19560.19749237190761879No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT18670.18850626705088155No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18170.18345789353586062No Hit
GTGCAGCCACAGTTCGTTTCATAGCCACTGTGGTCCCAGGGCCGAAACTG18130.18305402365465895No Hit
GTATGCTATCTCACCATTCAGTTTCGGCCCTGGGACCACAGTGGCTATGAAACGAACTGTGGCTGCACCAT18050.18224628389225558No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17350.1751785609712263No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17320.17487565856032503No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17040.17204856939191332No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16570.16730309828779363No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA16120.16275956212427478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16060.16215375730247228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16010.1616489199509702No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15950.1610431151291677No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15570.1572063512577518No Hit
GTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAATCTGTCTCAGACC15120.15266281509423293No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15070.15215797774273085No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATAGCCACTGTGGTCC14710.14852314881191578No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14620.14761444157921202No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG14520.14660476687620783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14440.1457970271138045No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTC14420.14559509217320363No Hit
GATAGCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGTTCCAGTCTGGTGATGGTGAGAGTGAAAT14370.14509025482170154No Hit
CTGCTACTCTGGCTCCCAGGTTCCACCGGAGAAGTTGTATTGACGCAGTC13930.14064768612848313No Hit
CCTGTAGGGCCAGTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACCAG13610.13741672707886973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13470.13600318249466387No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13240.13368093067775424No Hit
GTCTGAGACAGATTTCACTCTCACCATCACCAGACTGGAACCTGAAGACT13210.13337802826685297No Hit
TCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGAGACAGATTTCACT13090.13216641862324796No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13050.1317625487420463No Hit
GTCTCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCT12430.12550256558342032No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTTCCACCGGAGAAGTTGTATTGACGCAGTCTC12230.12348321617741197No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12180.12297837882590988No Hit
CACCATCACCAGACTGGAACCTGAAGACTTTGCAGTGTATTACTGTCAGCAGTATGCTATCTCACCATTCAGTTT12150.12267547641500862No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12070.12186773665260527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11990.12105999689020192No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11820.1193435498950948No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11750.11863677760299188No Hit
CCTGAAGACTTTGCAGTGTATTACTGTCAGCAGTATGCTATCTCACCATT11670.11782903784058853No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11530.11641549325638266No Hit
CTCCTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGTTCCTCATCTATAGTGCATCCAGCAGGGCCC11430.11540581855337846No Hit
GTCAGAGTCTTAGCAGAAACTCCTTAGCCTGGTACCAGCAGAAACCTGGC11180.11288163179586802No Hit
CTGTGGTCCCAGGGCCGAAACTGAATGGTGAGATAGCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGTT11150.11257872938496676No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10920.11025647756805713No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10670.10773229081054667No Hit
CAATAATAGACGGCCGTGTCGTCAGATTTCAGGCTCCTCAGCTCCATATA10630.10732842092934498No Hit
GCAGAAACCTGGCCAGCCTCCCAGGTTCCTCATCTATAGTGCATCCAGCAGGGCCCCTGGCATCCCAGACAGGTT10490.10591487634513913No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10390.10490520164213493No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10370.1047032667015341No Hit
CTATTATTGTGCCCGTGATTTCTTTTCGTCTAGTAGTGCATCACGTTACTGGGGCCAGGGCACCCTGGTCACCGT10260.10359262452822948No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10120.10217907994402363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10090.10187617753312239No Hit
GTCTGGAGCTGAGGTGAAGAAGGCTGGGGCCTCAGTGACAGTCTCCTGCAAGACTTCTGGTTACACCTTTACCAC10050.10147230765192071No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9930.10026069800831569No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9910.10005876306771483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4700.043.4174041
CTCCGGA555.8737947E-541.9637669
TTTGCGC405.9793485E-541.83918817
GTATCAA13600.038.3722761
AATGGTA4550.037.81759370
GTGGTAT1350.034.6917461
CAACGCA15000.034.3344235
ATCAACG15100.034.1070443
TCAACGC15100.034.1070444
TATCAAC15300.033.8814932
TTGCGCG502.2207884E-433.47811518
AACGCAG15400.033.447696
TGGTATC1400.033.444312
AACTGTG22400.033.2873670
TCACTAC552.5370377E-432.72717355
ACGCAGA16050.032.0979737
TAGCCGT552.9326545E-431.92894748
CGCAGAG16300.031.408378
AGAGTAC16700.031.05994411
GTGGCCG7850.031.05298270
Produced by FastQC (version 0.11.5)