FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268055
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38380.302668259657507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36280.2861074637929743No Hit
GTGTACATGGAGTTGAGCAGCCTGAGATCTGACGACACGGCCATTTATTACTGTGGGAGAACGTTTATTGGGAC28430.22420163163269732No Hit
GTATGTTACTTTACCTCACACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCAT27800.2192333928733375No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27500.21686756489268996No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27400.21607895556580747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24900.19636372239374475No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24410.19249953669202047No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT23980.18910851658642566No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23910.1885564900576079No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT22470.17720051575049978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22130.17451924403909927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21860.17238999885651646No Hit
GTAACATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC21840.17223227699113997No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTTTTAGCGG21530.1697875880778042No Hit
TTATTACTGTCAGCAGTATGTTACTTTACCTCACACTTTTGGCCAGGGGA21250.16757948196253317No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20960.1652925149145739No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20380.16071858081865534No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC18630.14691791759821143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18190.1434480365599284No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18150.1431325928291754No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18110.14281714909842239No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCG17930.14139765231003387No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17760.1400570164543336No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17570.13855865873325682No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17440.1375334666083096No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17400.13721802287755658No Hit
CCATGTACACTGTGCTCGTGGACGAGTCGCTGGTCATGGTGACTCTGCCCTGGAATTTCTGTGCGTAGGCCACAA17270.1361928307526093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17260.13611396981992105No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCCCCTGGCCAAAAGTG17040.13437902930077955No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16710.13177661852206726No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16560.1305937045317435No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16510.13019939986830223No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16270.1283067374837842No Hit
CAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16070.1267295188300192No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCC15780.12444255178205993No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15700.12381166432055392No Hit
CTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCA15640.12333849872442441No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15490.12215558473410064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15470.12199786286872415No Hit
CTCCTGCTGGGCCAGTCAGAGTTTTAGCGGCAGCTTCTTAGCCTGGTACC15460.1219190019360359No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15460.1219190019360359No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15410.12152469727259463No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCTGGGCCAGTCAGA15320.12081494887840039No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATGTTACTTTA15260.12034178328227088No Hit
GTATTATATGTTCTGGGTGCGACAGGCCCCTGGACAAGGTCTTGAGTGGA14940.11781823343624685No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13940.10993214016742177No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13440.10598909353300923No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA13240.10441187487924421No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13190.10401757021580293No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13160.1037809874177382No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13040.10283465622547919No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12910.10180946410053192No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12820.10109971570633766No Hit
GGCCAAAAGTGTGAGGTAAAGTAACATACTGCTGACAGTAATAAAGTGCA12780.10078427197558466No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12770.1007054110428964No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12710.1002322454467669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGC200.00834766850.1261149
CGTATGC4400.049.40822241
TCGTATG4550.046.8987640
CTTATAC4650.045.2841261
ATGCCGT4900.044.74002544
GCCGTCT4950.044.5552146
TATACAC4850.044.1041563
GTATGCC5000.042.91665642
CGTCTTC5200.041.98967748
TTATACA5100.041.942192
CCCACGA5150.041.5349819
ATCTCGT4950.041.36389537
CCGTCTT5350.041.34866347
TCTACAT5200.040.9042332
GGTATCA9050.040.625861
CTACATC5000.040.5922933
CACGAGA5350.039.99646821
TACACAT5350.039.9822735
ACGAGAC5200.039.87056422
ATACACA5650.039.6339764
Produced by FastQC (version 0.11.5)