FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268055
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46590.36741308539456097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37870.2986463520904062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31650.24959485195831413No Hit
GTGTACATGGAGTTGAGCAGCCTGAGATCTGACGACACGGCCATTTATTACTGTGGGAGAACGTTTATTGGGAC29530.23287633422840492No Hit
GTATGTTACTTTACCTCACACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCAT27930.2202585849982848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27400.21607895556580747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26720.21071641214300643No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25690.20259373607611655No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT24220.19100117897094368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22790.17972406559652382No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21860.17238999885651646No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGT21400.16876239595285694No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTTTTAGCGG20790.16395187905887365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20670.16300554786661461No Hit
TTATTACTGTCAGCAGTATGTTACTTTACCTCACACTTTTGGCCAGGGGA20650.16284782600123812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20420.16103402454940835No Hit
GTAACATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC20190.15922022309757858No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18490.14581386454057593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17990.14187081790616338No Hit
CCATGTACACTGTGCTCGTGGACGAGTCGCTGGTCATGGTGACTCTGCCCTGGAATTTCTGTGCGTAGGCCACAA17850.14076676484852785No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17480.13784891033906257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17410.13729688381024482No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCG17090.13477333396422078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.13469447303153254No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCCCCTGGCCAAAAGTG17030.13430016836809128No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC16970.13382700277196177No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16900.13327497624314402No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16500.13012053893561398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16190.12767585002227821No Hit
CAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16120.12712382349346046No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16090.1268872406953957No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15970.1259409095031367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15850.12499457831087768No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15820.12475799551281293No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15720.12396938618593042No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15510.12231330659947715No Hit
CTCCTGCTGGGCCAGTCAGAGTTTTAGCGGCAGCTTCTTAGCCTGGTACC15490.12215558473410064No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATGTTACTTTA15430.12168241913797113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15250.12026292234958262No Hit
CTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCA15170.11963203488807661No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCTGGGCCAGTCAGA14980.11813367716699985No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14870.11726620690742909No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14670.11568898825366407No Hit
GTATTATATGTTCTGGGTGCGACAGGCCCCTGGACAAGGTCTTGAGTGGA14570.11490037892678157No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14230.11221910721538103No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14040.11072074949430427No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13990.11032644483086301No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13730.10827606058096849No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCC13710.10811833871559198No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA13520.10661998099451522No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13080.10315009995623219No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12950.10212490783128492No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12880.10157288130246717No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12820.10109971570633766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.040.9899751
ATATTAC601.6786214E-533.3926961
GTATCAA22150.033.3173181
TATCAAC24950.029.1687322
AATGGTA6550.029.07700370
TGTTAGA12550.028.10305470
ATCAACG27050.026.9042433
TCAACGC27250.026.5842744
AACGCAG27750.026.232826
CAACGCA27550.026.1756785
ACGCAGA28950.025.1523957
CGCAGAG28900.025.0833028
GAGTTTA4550.024.9527821
AGAGTAC29150.024.64870611
AGTTTAT4750.024.5983522
TACGGGT4500.024.51021215
TATACTG851.8032336E-423.5666665
TAGGCAA3902.7284841E-1123.51288870
GTAGTTT1852.203019E-422.87740370
CGCCACG10350.022.49058770
Produced by FastQC (version 0.11.5)