FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269160
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53780.4237448391061805No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51640.40688329288663366No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42600.33565507894985663No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37750.29744082700368746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35380.2787670585268997No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33200.26159034321913704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32500.25607488417536006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32090.2528444010211478No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCG31600.24898357969050394No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31110.24512275835986008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30470.24008005294840684No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAATTATTAACAGCTGGTTGGCCTGGTATCAGCAGAAA30090.23708594661035645No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29790.23472217844873774No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29690.23393425572819818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27840.21935768539821615No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27760.21872734722178447No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27640.217781839957137No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27220.2144725645308708No Hit
GCATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGC26950.212345173185414No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26480.20864193639887801No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26400.20801159822244633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23730.18697406158403984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23210.18287686343723408No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23180.1826404866210722No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT22840.17996154937123765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGAAA22390.1764158971288096No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTCCCTCGGACGTT21790.17168836080557218No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21310.16790633174698225No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20720.16325758769579882No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20180.15900280500488512No Hit
GATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACC19500.15364493050521605No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT19260.15175391597592108No Hit
GGCTCACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19140.1508084087112736No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18940.14923256327019446No Hit
GTTCAGCGGCAGTGGATCTGGGACAGATTTCACGCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA18540.1460808723880362No Hit
GTCTCCAACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG18280.1440322733146333No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18000.14182608969712251No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17890.140959374704529No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17600.13867439881496424No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTT17140.13504995430048222No Hit
CCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG16970.13371048567556496No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC16870.1329225629550254No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAATTATTAACAG16840.13268618613886352No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16610.13087396388162248No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16580.1306375870654606No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTGAGGATCA16540.1303224179772448No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16530.13024362570519082No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16510.1300860411610829No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16440.12953449525670524No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACGCTCA16280.1282738189038419No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16000.1260676352863311No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15800.12449178984525197No Hit
GGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT15750.12409782848498219No Hit
CTGTGAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGCGTGAAATCTGTC15700.1237038671247124No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15580.12275835986006493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15550.12252198304390305No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15360.12102492987487788No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15200.11976425352201456No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15200.11976425352201456No Hit
GCTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCGTG15100.11897633080147499No Hit
ATTGTCAACAGGCTCACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG14890.1173216930883419No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14750.1162186012795865No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14720.11598222446342463No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14660.11550947083110087No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14640.11535188628699297No Hit
GCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTGTTA14630.11527309401493903No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14520.11440637902234549No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14270.11243657222099657No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14200.11188502631661887No Hit
CAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14160.11156985722840303No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14150.11149106495634908No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14010.11038797314759367No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13960.10999401178732389No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13920.10967884269910808No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13890.1094424658829462No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13640.10747265908159728No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13290.10471492955970879No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13240.10432096819943901No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC13240.10432096819943901No Hit
GATTTCACGCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTC13060.10290270730246777No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC12850.10124806958933467No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGAAACTGTGAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG12700.10006618550852532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG250.0070066452.3956769
CGTATGC3250.045.65369441
CTTATAC4150.044.8926931
AGACGTA4350.044.4333325
TATACAC4300.044.1161653
CGAGACG4350.043.63142823
TCGTATG3400.043.5678740
GACGTAG4400.043.17135226
ACGAGAC4450.042.64758722
ACGTAGA4550.042.51542727
GTCGTAT651.1703378E-842.32925439
CACGAGA4600.041.98866721
TTATACA4650.040.0670972
CGTAGAG4800.039.6128128
TACACAT4800.039.522295
AATCTCG3350.038.8013336
ATCTCGT3150.038.0601537
TTGGTCG556.938828E-637.98711846
ACACATC5200.037.788026
ATGCCGT3950.037.75102644
Produced by FastQC (version 0.11.5)