FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112324
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCAT78330.7042012938676141No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC64750.582114563742219No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51710.4648825342256393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46320.4164254300006114No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39600.35601137797979726No Hit
GTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTCGTAGCTGGTTGGCCTGGTATCAGCAGAAG38630.34729089725655476No Hit
GTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA36430.32751248736878824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31500.283190868847566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30390.2732117620405565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28700.25801834717222677No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27680.24884835713335324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27630.24839884781772217No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27080.24345424534578056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26580.23895915218946998No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24720.22223740564799466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24600.2211585832904801No Hit
CCTTCCACCCTGTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCG24520.2204393683854704No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24500.22025956465921798No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23870.21459574728226669No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22930.20614497214840277No Hit
GAGTATTCGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTC21970.19751439328828652No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21690.1949971411207526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21660.19472743553137395No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21590.1940981224894905No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCC21230.19086165541694686No Hit
TAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTT20680.18591705294500524No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19810.17809559085302484No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19470.17503892750673364No Hit
CAGTATAATAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGC19360.17405000701234533No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19330.1737803014229667No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19250.17306108651795699No Hit
GTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCCCTTGGCCGAACGTC18930.17018422689791823No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT18850.16946501199290853No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18390.1653295262891028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17830.16029502195403497No Hit
GGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17820.16020512009090876No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17710.15921619959652045No Hit
GAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG17660.15876669028088938No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA17550.15777776978650107No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17420.1566090455658603No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17220.15481100830333608No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17160.1542715971245788No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17090.15364228408269534No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16570.14896738720013233No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGAATAACTATTATACTGTTGG15460.13898828039312286No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTCGTAG15120.13593161704683168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14840.13341436487929775No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14790.1329648555636667No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14710.13224564065865702No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14600.13125672016426868No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14480.13017789780675415No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGC14460.12999809408050172No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14440.1298182903542493No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14310.12864956613360856No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14130.12703133259733676No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14040.12622221582920085No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13950.12541309906106496No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13940.12532319719793872No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCGTGGACGTTCGGCCA13850.12451408042980282No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13810.124154472977298No Hit
GTCTCGTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG13580.12208673012539512No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC13350.12001898727349225No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13340.11992908541036604No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13220.11885026305285151No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13020.11705222579032727No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA12990.11678252020094866No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG12970.11660271647469622No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12940.11633301088531758No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG12840.11543399225405547No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12820.11525418852780306No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12750.11462487548591957No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12580.11309654381277397No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC12500.11237732890776429No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12400.11147831027650218No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC12280.11039948791898764No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12160.10932066556147309No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12080.10860145065646341No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11970.10761253016207507No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11700.10518517985766737No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11560.10392655377390041No Hit
GCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAGCTACGA11390.1023982221007548No Hit
GCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTC11300.1015891053326189No Hit
GCTGATACCAGGCCAACCAGCTACGAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCGTACAGATG11230.10095979229073544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT501.1459633E-955.031939
ATCGCGT501.1859811E-954.82381437
CCCACGA3200.046.50407819
TCGTATG1950.045.95237740
TCGCGTA605.8789738E-945.75738538
CGAGACG3250.044.77012623
ATCTCGT1650.043.6098537
CGTATGC2400.043.18721441
CGCTCAT3600.042.4003628
GCCCACG3550.041.91728618
ATGCCGT2500.041.7010844
CACGAGA3550.040.9719921
GACGCTC3800.040.1199126
TCTCCGA3900.039.88084811
CATGAAT3700.039.5689932
GCCGTCT2300.039.43850746
GCGTATG702.2091626E-839.3877540
CTTATAC4050.039.2421761
GAGACGC4250.036.62782724
TTATACA4350.036.5358162
Produced by FastQC (version 0.11.5)