FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112324
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCAT75270.6766913237509934No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC64000.5753719240077532No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50170.45103764730420276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36180.32526494079063295No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35220.31663436193051664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33220.29865398930527437No Hit
GTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTCGTAGCTGGTTGGCCTGGTATCAGCAGAAG32320.29056282162391533No Hit
GTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA30650.275549210481838No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29850.26835706143174115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28750.25846785648785786No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27300.2454320863345572No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25320.2276315174355673No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24810.22304652241613054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24650.22160809260611114No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA24200.21756250876543165No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23100.20767330382154842No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21730.1953567485732574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21620.1943678280788691No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21400.19238998709009245No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21290.19140106659570413No Hit
CCTTCCACCCTGTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCG21060.18933332374380127No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20420.18357960450372374No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20290.182410880283083No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20230.18187146910432572No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20060.18034313743118013No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19850.17845519830552967No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19470.17503892750673364No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18920.17009432503479202No Hit
GAGTATTCGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTC18700.16811648404601537No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18630.16748717100413188No Hit
TAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTT18550.1667679560991222No Hit
GTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCCCTTGGCCGAACGTC18240.16398099834220964No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTGCCACCTTGGTCC18070.16245266666906405No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17330.15579992879772442No Hit
CAGTATAATAGTTATTCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGC17230.1549009101664623No Hit
GAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG17160.1542715971245788No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16820.15121493377828762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16350.14698954621135568No Hit
GGACCAAGGTGGCAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16260.14618042944321977No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT15970.14357327541255965No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15440.13880847666687043No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15390.13835896735123937No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15060.1353922058680744No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14880.1337739723318026No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14780.1328749537005405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14170.12739094004984158No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14100.12676162700795812No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC14030.12613231396607463No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13990.12577270651356978No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13790.12397466925104556No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13780.12388476738791936No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13770.12379486552479314No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13670.12289584689353102No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTACGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTCGTAG13660.1228059450304048No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13650.1227160431672786No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13470.12109780963100679No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGAATAACTATTATACTGTTGG13470.12109780963100679No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13420.12064830031537574No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13080.11759163696908453No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCGTGGACGTTCGGCCA12970.11660271647469622No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG12600.1132763475390264No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12570.11300664194964775No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12550.11282683822339534No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12530.11264703449714292No Hit
TCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGC12520.1125571326340167No Hit
GTCTCGTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG12450.11192781959213323No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12290.11048938978211384No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12000.10788223575145373No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11910.10707311898331781No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11610.10437606308953146No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11530.10365684818452177No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG11490.10329724073201695No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11320.10176890905887134No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11140.10015067552259953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC352.168235E-549.67978344
GCCTACG350.001057203941.42422561
TTTACGC350.001304921939.68862543
GTGGTAT2750.035.5983281
GTATCAA29300.034.2178761
GATTCAC2400.033.7570341
ATAGGCC553.7218447E-430.657868
ACTATCG450.00480588530.42384137
GCGACGG450.005120483730.03192720
GTATAGA450.005141946530.0062481
TATCAAC33350.029.5417582
ATCAACG33900.029.248373
CCTACGA500.00597504929.09819262
ACAATGG4100.029.07303270
TCAACGC34500.028.443884
CAACGCA35050.028.286185
AACGCAG35400.028.1970336
AACGAAG606.19588E-428.1017746
GACAGGT2100.027.3271221
CGCAGAG37250.026.7073158
Produced by FastQC (version 0.11.5)