FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155038
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44760.387519717965989No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36240.3137559110609348No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC31290.27090017817595613No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAATCTGAAGATT30460.2637142674093839No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29810.25808674692953826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28560.24726459216060423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27660.23947264072697177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25830.22362900614525238No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23850.2064867129912609No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23410.2026773145125961No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT23260.20137865594032403No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAATCTGAAGATTCTGCAGTTTATTACTGTCACCAGTATCATAATTGG23210.20094576974956668No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23190.20077261527326373No Hit
GTATTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAACGAGCCACCCTCTT22850.1978289891761137No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTCGT22550.19523167203156952No Hit
GTTTATTACTGTCACCAGTATCATAATTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA21810.18882495640836058No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAACGAGCCACCCTCTTCT21730.1881323385031488No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21390.18518871240599877No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20850.18051354154581925No Hit
GTATCATAATTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG20630.17860884230648688No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGGCCAATTATGATA20390.17653098859085156No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACGAGGGCCACTGGTATCCCA19800.1714229315399147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19760.1710766225873088No Hit
CCCTTGTCCCGTGGCCCCAGACATCAAAAGCCAACGATAGCAGGTGGATCTGTTCTCTCACACAGAAATATACAG19730.1708168908728544No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19630.16995111849133968No Hit
TTATGATACTGGTGACAGTAATAAACTGCAGAATCTTCAGATTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTC19210.16631487448897786No Hit
CACCATCACCAGCCTGCAATCTGAAGATTCTGCAGTTTATTACTGTCACCAGTATCATAATTGGCCTCCGGGGTA19080.1651893703930087No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA18680.16172628086694982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18650.16146654915249542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18250.15800345962643653No Hit
GGCCAAAAGTGTACCCCGGAGGCCAATTATGATACTGGTGACAGTAATAAACTGCAGAATCTTCAGATTGCAGG18060.15635849210155855No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17990.15575245143449826No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17970.15557929695819533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGG17840.15445379286222616No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17600.15237593914659084No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17480.1513370122887732No Hit
GTAATAAACTGCAGAATCTTCAGATTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA17410.1507309716217129No Hit
GTCTGTGTCTCCAGGGGAACGAGCCACCCTCTTCTGCAGGGCCAGTCAGACTGTTAGCAGAAACTTAGCCTGGTA16990.14709472761935105No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16830.14570949180892748No Hit
CTTCTGCAGGGCCAGTCAGACTGTTAGCAGAAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT16480.142679288473626No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16390.14190009333026274No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTTCTGCTA16180.14008197132908182No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15650.1354933777070538No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15610.1351470687544479No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15600.13506049151629643No Hit
CATCTATGGTGCATCCACGAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA15540.1345410280873876No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15420.13350210122956993No Hit
GGACAAGGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTC15190.1315108247520861No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15150.13116451579948019No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA14840.12848062141678457No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14640.12674907665375512No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC14630.12666249941560365No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14610.1264893449393007No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14360.1243249139855139No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14170.12267994646063593No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14050.12164101960281826No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13990.12112155617390943No Hit
CTCCTGTGCAGCCTCAGGATTCAGCTTCAGTAACTATACCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG13870.12008262931609177No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13610.11783162112415349No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13510.11696584874263877No Hit
GGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT13480.11670611702818436No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13450.11644638531372994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13450.11644638531372994No Hit
GGATACCAGTGGCCCTCGTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG13450.11644638531372994No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13240.11462826331254902No Hit
CTCCAGAGACAATGCCAACAATTCAGTGTATTTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTT13210.1143685315980946No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13210.1143685315980946No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13090.11332960474027694No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13040.11289671854951959No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12990.11246383235876223No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12720.11012624692867247No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12710.110039669690521No Hit
TCTATGGTGCATCCACGAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12460.10787523873673421No Hit
GAGTTGGGGCTGTGCTGGATTTTCCTTGTTGCTATTTTAGAAGGTGTCCGGTGTGACGTGCAGCTGGTGGAGT12420.10752892978412833No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12370.10709604359337097No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11830.10242087273319148No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11600.1004295962557076No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11570.10016986454125319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAA208.737288E-568.6404934
GGTATCA7450.046.2461131
GTGGTAT2050.039.9362751
GTATCAA20250.039.0816731
AATGGTA7050.034.88707470
TATCAAC22900.034.4101562
TCAACGC23850.032.8964964
ATCAACG24000.032.690893
CAACGCA24200.032.561685
AACGCAG24800.031.7738976
ATATAGC553.4801973E-431.0111246
CGCAGAG25800.030.5423498
AGAGTAC26200.030.20625311
TGTTAGA11700.029.87293870
TAGTCAC1052.3695975E-829.52150538
GTAATAG605.792913E-428.426861
GAGTTAA605.792913E-428.426861
GCAGAGT28000.028.2644239
CCGAAAT500.00741248227.84182747
TTTCGGC500.00815795827.30042523
Produced by FastQC (version 0.11.5)