FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155038
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45030.38985730339607877No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31040.2687357472221693No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC30560.2645800397908986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29460.25505654359423674No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAATCTGAAGATT29310.2537578850219646No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27210.2355766650101555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26510.22951625833955244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25860.22388873785970678No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAATCTGAAGATTCTGCAGTTTATTACTGTCACCAGTATCATAATTGG24030.2080451032779874No Hit
GTATTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAACGAGCCACCCTCTT23250.20129207870217256No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22190.19211489145811653No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22140.19168200526735918No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTCGT21850.18917126536096646No Hit
GTATCATAATTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG21690.1877860295505429No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21550.18657394821642231No Hit
GTTTATTACTGTCACCAGTATCATAATTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA21300.18440951726263552No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20550.1779162244012751No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20540.17782964716312363No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGGCCAATTATGATA20220.1750591755422765No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAACGAGCCACCCTCTTCT19970.17289474458848975No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19840.17176924049252057No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19600.16969138677688525No Hit
TTATGATACTGGTGACAGTAATAAACTGCAGAATCTTCAGATTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTC18680.16172628086694982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18610.16112024019988952No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18530.16042762229467775No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACGAGGGCCACTGGTATCCCA17950.15540614248189238No Hit
GGCCAAAAGTGTACCCCGGAGGCCAATTATGATACTGGTGACAGTAATAAACTGCAGAATCTTCAGATTGCAGG17900.154973256291135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17740.15358802048071146No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17670.15298197981365114No Hit
CACCATCACCAGCCTGCAATCTGAAGATTCTGCAGTTTATTACTGTCACCAGTATCATAATTGGCCTCCGGGGTA17420.15081754885986437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17390.15055781714540994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17180.14873969514422902No Hit
CCCTTGTCCCGTGGCCCCAGACATCAAAAGCCAACGATAGCAGGTGGATCTGTTCTCTCACACAGAAATATACAG17120.1482202317153202No Hit
GTAATAAACTGCAGAATCTTCAGATTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA17090.14796050000086577No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16860.14596922352338193No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA16690.14449741047480688No Hit
GTCTGTGTCTCCAGGGGAACGAGCCACCCTCTTCTGCAGGGCCAGTCAGACTGTTAGCAGAAACTTAGCCTGGTA16250.14068801199614211No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16110.13947593066202152No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15900.1376578086608406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCCCGGAGG15850.13722492247008325No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15790.1367054590411744No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15750.1363591500885685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15620.13523364599259938No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15480.13402156465847878No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15440.13367525570587288No Hit
CTTCTGCAGGGCCAGTCAGACTGTTAGCAGAAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACT15210.131683979228389No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTTCTGCTA15140.1310779385613287No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15020.13003901170351106No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14820.1283074669404816No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14780.12796115798787572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14500.1255369953196345No Hit
CATCTATGGTGCATCCACGAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14220.12311283265139328No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14160.12259336922248446No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14010.12129471065021238No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13980.12103497893575796No Hit
CTCCTGTGCAGCCTCAGGATTCAGCTTCAGTAACTATACCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG13780.11930343417272851No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13650.11817793007675938No Hit
GGACAAGGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTC13540.11722558045709319No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13410.11610007636112406No Hit
CTCCAGAGACAATGCCAACAATTCAGTGTATTTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTT13340.11549403569406376No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13050.11298329578767105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13020.11272356407321664No Hit
GGATACCAGTGGCCCTCGTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG12880.11151148273909604No Hit
CTGCTACTCTGGCTCCCAGATAGTATTGGAGAAATAGTGATGACGCAGTC12700.10995309245236953No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12680.10977993797606658No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12490.10813497045118863No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12370.10709604359337097No Hit
GAGTTGGGGCTGTGCTGGATTTTCCTTGTTGCTATTTTAGAAGGTGTCCGGTGTGACGTGCAGCTGGTGGAGT12170.1053644988303415No Hit
GGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT11760.10181483206613115No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11730.10155510035167674No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11640.10077590520831349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.045.8875431
GGTATCA7250.044.1087881
ACGCTAT359.642114E-442.2067667
TACGCTA350.00104104641.5542766
TATACGC350.001097958341.10714364
CGGGTAC603.2343632E-739.71244417
GTATCAA22300.039.3595471
CGAGCTT350.001361901939.34525337
TACGTAT859.17657E-538.90323370
TATCAAC23000.038.150082
ATCAACG23450.036.836273
TCAACGC23900.036.14274
AGTTAGT400.002077820536.10264665
CAACGCA24150.035.77635
AACGCAG24650.035.055176
ACGGGTA1300.034.04218716
CGCAGAG25950.033.441728
AGAGTAC26250.033.19923811
GCAGAGT26900.031.8866779
CGGACCG553.538523E-430.923325
Produced by FastQC (version 0.11.5)