FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856615
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23110.2697828079125395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21600.25215528563006717No Hit
ATGTATTACTGTGCGAAAGAGCAAACGGTGGTGACAAAGTACTACTTTGA19950.23289342353332593No Hit
GGTCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCA19330.22565563292727772No Hit
GTAATACATAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTCCAGATACA18400.21479894701820537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18210.2125809144131261No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17590.20534312380707784No Hit
GGCTAACAGATTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16610.19390274510719518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15980.18654821594298487No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14940.17440740589412979No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.16250007296159885No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCT13890.16214985728711265No Hit
GCATAGACCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC13630.1591146547748989No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13570.15841422342592648No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13170.15374468109944375No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA13140.15339446542495752No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13110.15304424975047135No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13090.1528107726341472No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12940.15105969426171617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12270.1432382108648576No Hit
GTATTACTGTGCGAAAGAGCAAACGGTGGTGACAAAGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC12210.1425377795158852No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12190.14230430239956104No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11970.13973605411999557No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11780.13751802151491627No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11640.1358836817006473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11510.13436608044454043No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11490.1341326033282163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11430.1334321719792439No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11350.13249826351394733No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGGAAT11140.1300467537925439No Hit
GAGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGGTCTATGCTGCATC11060.12911284532724737No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10890.1271282898384922No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10860.126778074164006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10760.1256106885823853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10500.12257548607017155No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10360.12094114625590259No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10220.11930680644163365No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGCCATAACTAC10170.1187231136508233No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10010.11685529672023022No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9820.11463726411515092No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9700.11323640141720609No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9690.11311966285904403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9680.11300292430088195No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9610.11218575439374749No Hit
CAGTAATACATAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTCCAGATA9510.11101836881212679No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGTTATGGCATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGG9470.11055141457947852No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9130.1065823036019682No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9030.10541491802034753No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.10494796378769926No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8970.10471448667137512No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCACTCTCA8940.10436427099688893No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8870.10354710108975444No Hit
GTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT8730.10191276127548547No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8640.10086211425202687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.6336253E-676.8180170
CGTATGC750.050.58198541
TCGTATG850.048.60160440
GTACGTA551.2742912E-648.5963369
GGTACGT552.8182676E-643.30643568
GTGGTAT2150.042.3975371
ACGAGAC1000.040.55344822
ATCTCCG957.2759576E-1239.09625210
GACAGGT1050.038.5840031
TCTATAC350.001498948838.5843
ATGCCGT1009.094947E-1238.1869644
CGAGGCT1001.0913936E-1137.30258629
CACGAGA1052.0008883E-1135.40173321
CCGTCTT1102.3646862E-1134.91660347
ATCTCGT807.5531716E-834.25284237
AGACCGA1201.8189894E-1233.81429725
CCCACGA1103.6379788E-1133.78466419
TCCTATA1300.033.7614
GAGACCG1253.6379788E-1232.4503424
GTATCAA18450.032.0226331
Produced by FastQC (version 0.11.5)