FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281965
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCGACAATAAGAACTTCTTAGCTTGGTATCAGCAGAA87260.6806738093473691No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71670.5590636249819613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64600.5039139134063723No Hit
GTATTATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT62650.48870288970447706No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACACCCAGGGAGTCTGGAGA53820.41982425417230584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52660.4107756452009221No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48600.3791055138010788No Hit
TTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTC43610.3401808941741779No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41210.3214596342333839No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40110.31287905676052No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40050.3124110252620001No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39840.31077291501718063No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39000.30422047403790275No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36700.2862792665946418No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34320.2677140171533544No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33220.2591334396804905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33160.25866540818197065No Hit
CCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC32120.25055286220762657No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31110.24267433198254243No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTAAC31000.24181627423525603No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30800.24025616924018986No Hit
ATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA30440.23744798024907077No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30100.23479580175745826No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29850.2328456705136256No Hit
ACTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTATTGTCGGAGCTGTATAAAACACT29520.23027149727176638No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG29500.2301154867722598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28260.22044283580284954No Hit
TTATTACTGTCAGCAGTATTATAATATTCCATTCACTTTCGGCCCTGGGA28110.21927275705654992No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGATTTCTGCTGATA27920.21779065731123703No Hit
GTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA27690.21599653656691092No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26010.20289165460835515No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25740.2007855128650158No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT25440.19844535537241656No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCA24640.1922049353921519No Hit
CTTTATTACTGTCAGCAGTATTATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA24600.19189291439313866No Hit
AATCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA24570.19165889864387872No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTAACCATCAGCAGCC24500.19111286189560556No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24030.18744661515720007No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22520.1756678224444505No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22440.17504378044642405No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGACAATAAGAACTT22220.17332766495185126No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21930.1710655127090053No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21780.16989543396270568No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCA21320.1663071924740535No Hit
CTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGA21260.16583916097553364No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20640.16100283549082853No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19900.15523044700908373No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19820.15460640501105724No Hit
GTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTATTGTCGGAGCTG19790.1543723892617973No Hit
ATTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAAT19500.15211023701895138No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTA19020.14836598503079257No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18850.14703989578498633No Hit
CTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTAT18830.14688388528547972No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18560.1447777435421404No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18430.1437636752953474No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18420.14368567004559407No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17990.14033144430620179No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17840.1391613655599022No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17370.1354951188214967No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17370.1354951188214967No Hit
GATTTCACTCTAACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTATAATATT17340.13526110307223677No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17000.13260892458062426No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16970.13237490883136435No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16700.13026876708802504No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16680.1301127565885184No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16600.12948871459049194No Hit
GAATATTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAAT16500.12870866209295886No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16410.12800661484517908No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16320.1273045675973993No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16160.1260564836013464No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16000.12480839960529344No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15970.12457438385603352No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT15770.12301427886096734No Hit
CTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG15610.12176619486491441No Hit
GTCTGGGACAGATTTCACTCTAACCATCAGCAGCCTGCAGGCTGAAGATG15520.12106414761713465No Hit
GGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTATAATATTCCAT15420.12028409511960156No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15390.12005007937034164No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15310.11942603737231515No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15270.11911401637330192No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA15110.11786593237724897No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT14990.11692986938020929No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14880.1160718116329229No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14760.11513574863588319No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14680.11451170663785673No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14460.11279559114328395No Hit
CTTCTTAGCTTGGTATCAGCAGAAATCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATC14240.11107947564871117No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13810.1077252499093189No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13730.10710120791129242No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13550.10569711341573287No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13470.10507307141770642No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13320.10390299267140678No Hit
GTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGT13220.10312294017387369No Hit
CACCCAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCA13140.10249889817584723No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13120.1023428876763406No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12980.1012508141797943No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGACAATAA12930.10086078793102775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)