FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281965
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCGACAATAAGAACTTCTTAGCTTGGTATCAGCAGAA75280.5872235201429057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72910.5687362759513716No Hit
GTATTATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT62810.48995097370053003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53300.4157679811851338No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52950.413037797443768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50370.3929124430074144No Hit
TTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTC47060.36709270533906935No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACACCCAGGGAGTCTGGAGA45490.3548458811277999No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42230.32941616970822135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40130.3130350672600266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38560.3007882430487572No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38120.2973560120596116No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38030.2966539648118318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35910.28011685186413043No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32290.2518789514534328No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30970.2415822584859961No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG30830.24049018498944977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30360.23682393825104428No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30280.23619989625301782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30240.2358878752540046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29910.23331370201214544No Hit
ATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA29520.23027149727176638No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCA29340.22886740277620685No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTAAC28690.2237970615422418No Hit
CCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC27790.21677658906444405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27750.2164645680654308No Hit
TTATTACTGTCAGCAGTATTATAATATTCCATTCACTTTCGGCCCTGGGA27380.2135783738245584No Hit
ACTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTATTGTCGGAGCTGTATAAAACACT27010.210692179583686No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26910.20991212708615292No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCA26670.2080400010920735No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26480.20655790134676064No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26340.20546582785021433No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT26140.20390572285514816No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25900.20203359686106875No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGATTTCTGCTGATA25340.1976653028748835No Hit
GTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA24450.19072283564683903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24210.18885070965275963No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23510.18339034217002803No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23170.18073816367841555No Hit
CTTTATTACTGTCAGCAGTATTATAATATTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA22950.1790220481838428No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT22290.17387370170012442No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21840.17036346546122552No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21590.16841333421739282No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21450.1673212607208465No Hit
ATTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAAT21290.16607317672479358No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTAACCATCAGCAGCC21070.16435706123022079No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20540.16022278299329545No Hit
AATCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA19010.14828797978103928No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18940.1477419430327661No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18810.1467278747859731No Hit
CTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGA18780.14649385903671316No Hit
GAATATTATAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTTAGAGTGAAAT18660.14555779603967348No Hit
GTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTATTGTCGGAGCTG18620.14524577504066025No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTA18600.1450897645411536No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGACAATAAGAACTT18490.14423170679386724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18350.1431396332973209No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18350.1431396332973209No Hit
CTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTAAGAAGTTCTTAT18240.1422815755500345No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18200.14196955455102128No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18100.1411895020534882No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17850.13923937080965548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGAATATT17510.136587192318043No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17270.1347150663239636No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.13393501382643053No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17060.13307695607914413No Hit
GATTTCACTCTAACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTATAATATT16940.13214089308210442No Hit
GGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTATAATATTCCAT16840.13136084058457134No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16830.13128283533481805No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16720.13042477758753163No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16400.12792860959542576No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16060.12527643110381328No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16040.12512042060430667No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15770.12301427886096734No Hit
GTCTGGGACAGATTTCACTCTAACCATCAGCAGCCTGCAGGCTGAAGATG15670.12223422636343426No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA15260.11903601112354863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15060.11747590612848245No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14990.11692986938020929No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT14710.11474572238711665No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGGTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14560.11357564364081704No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14460.11279559114328395No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14360.11201553864575087No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14120.11014341265167145No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14040.10951937065364499No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT13900.10842729715709867No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13890.10834929190734535No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13630.10632115541375936No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13450.10491706091819979No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13380.10437102416992664No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13190.10288892442461377No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12920.10078278268127445No Hit
GTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGT12850.10023674593300129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)