FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443934
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65920.45653056164616945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62160.4304905902901379No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49230.3409435611322955No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGG48520.33602643888155553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48460.3356109074237465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41510.2874785135608691No Hit
GCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTG40390.2797219263484342No Hit
ATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGA39960.27674395090080295No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAA38770.26850257698759084No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA37970.26296215755013735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37940.26275439182123284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37460.2594301401587607No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37320.25846056675720636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37230.25783726957049286No Hit
GTAGTACACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGAAACA36160.25042695857289876No Hit
GCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGG35130.24329366854717738No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA34880.24156228747297315No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34550.23927686445502355No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33820.23422123171834724No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG33250.23027368286916164No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32260.22341741381531288No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA31960.2213397565262678No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31500.21815401534973203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31140.21566082660287797No Hit
GTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT30820.2134446588278966No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA30820.2134446588278966No Hit
CCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC30620.21205955396853318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30230.20935859949277458No Hit
ACATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA29910.2071424317177932No Hit
GATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTG29400.20361041432641658No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC29180.20208679898111687No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29010.200909459850658No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28580.19793148440302674No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCT27210.1884435161163876No Hit
GGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATC26890.1862273483414062No Hit
CACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGACCCCT26750.18525777493985182No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26710.18498075396797914No Hit
CAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT26600.18421894629532928No Hit
GTGTACTACTGTGCGAAAGGTCAGAAGCAATGGCTGGTAGGAAGGTTTGA25590.17722416675554423No Hit
GATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG25020.1732766179063586No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTC24970.17293034169151775No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT23570.1632346076759741No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23560.16316535243300595No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23250.1610184399009927No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22980.15914854834085215No Hit
TTTTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTC22580.15637833862212538No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22510.1558935519213482No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21780.15083791918467188No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG21350.14785994373704062No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20900.144743457803473No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCT20490.14190399284177807No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATACATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG20070.138995272637115No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19820.13726389156291077No Hit
GTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGA19720.13657133913322908No Hit
ACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACA19670.13622506291838823No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19420.134493681844184No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19330.1338703846574705No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19200.1329700664988843No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18690.12943804910750767No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18660.12923028337860318No Hit
CCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAG18620.1289532624067305No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18140.1256290107442584No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17890.12389762967005416No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA17780.12313582199740432No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17690.12251252481069079No Hit
CATCAAGGTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTG17590.1218199723810091No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17580.12175071713804092No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17550.12154295140913644No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17480.12105816470835926No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17370.12029635703570939No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG17360.12022710179274121No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTGCCCC17260.11953454936305954No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17020.11787242353182349No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16890.11697210537323728No Hit
ACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG16750.11600253197168292No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16740.11593327672871476No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGGTA16740.11593327672871476No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16720.11579476624277842No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16710.11572551099981025No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16700.11565625575684207No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16650.11530997954200123No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16570.11475593759825586No Hit
GGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCT16510.11434040614044685No Hit
ATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGAC16290.11281679079514714No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16270.1126782803092108No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15950.11046211253422941No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15920.11025434680532491No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTT15740.10900775243189785No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15740.10900775243189785No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATTGCTAC15730.10893849718892967No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15500.1073456266006618No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA15440.1069300951428528No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15430.10686083989988462No Hit
CCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAAACAGGGGTCCCA15300.10596052174129843No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15250.10561424552645758No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15190.10519871406864856No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14960.10360584348038067No Hit
CCACTGAACCTTGATGGGACCCCTGTTTCCAAATTGGATACATCGTAGAT14950.1035365882374125No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCA14820.10263627007882632No Hit
GTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCC14580.10097414424759027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT200.007058261452.2996849
TGATACG200.007196656452.04348845
TCGTATG352.2769671E-549.2717840
GATACGC306.1012566E-446.3180146
TCTGGAG10950.046.0011970
GTGGTAT3200.043.6825641
CTACGCT1250.038.28172728
ACTACGC1250.038.2538427
ATCTCGT557.5826993E-637.49960337
GTATCAA30900.037.072771
TACGCTG1300.036.8195829
ACGAGAC1350.035.36378522
CGTATGC601.3312387E-534.5434941
ATCAACG34300.033.298523
TATCAAC34400.033.201722
GACAGGT1850.033.1722561
TCAACGC34550.033.0575754
CTTATAC1550.032.993911
CGAGACT1450.032.93060723
TGTTAGA18950.032.60622870
Produced by FastQC (version 0.11.5)