FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443934
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63860.44226398159472663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50310.34842312737285774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48370.334987610237033No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGG47920.3318711243034654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47240.3271617677816299No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38160.26427800716653255No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37820.26192332890561476No Hit
GCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTG35660.2469641964244903No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAA35130.24329366854717738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34810.24107750077219597No Hit
ATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGA34640.23990016164173708No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34060.23588335754958328No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA33320.23075846956993878No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG32110.22237858517079037No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32050.22196305371298136No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA31830.22043943836768162No Hit
GCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGG31680.21940060972315908No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31400.2174614629200504No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31340.21704593146224135No Hit
GTAGTACACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGAAACA31310.21683816573333684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30410.21060519386620163No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA29280.2027793514107986No Hit
CCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC28960.20056318363581715No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA28930.20035541790691266No Hit
GTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT28170.1950920194413318No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28060.19433021176868195No Hit
GATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTG27780.19239106496557323No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27630.19135223632105067No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27350.18941308951794195No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27180.18823575038748308No Hit
ACATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA27060.18740468747186506No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC26830.18581181688359716No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26810.18567330639766083No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24930.1726533207196451No Hit
CAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT24560.17009087672982284No Hit
CACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGACCCCT24310.1683594956556186No Hit
GGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATC24280.1681517299267141No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24220.16773619846890508No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCT23290.1612954608728654No Hit
GTGTACTACTGTGCGAAAGGTCAGAAGCAATGGCTGGTAGGAAGGTTTGA22850.15824823018226594No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22500.15582429667838005No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22210.15381589463230314No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTC22160.1534696184174623No Hit
GATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG22080.15291557647371692No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22010.15243078977293978No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT21890.15159972685732173No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG21530.14910653811046765No Hit
TTTTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTC20770.14384313964488682No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20540.14225026905661892No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCT20430.1414884613839691No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19990.13844123069336964No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19060.13200049309732995No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18830.13040762250906204No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18700.12950730435047586No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATACATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG18590.128745496677826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18490.1280529442481443No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18420.1275681575473671No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18350.12708337084658994No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18160.12576752123019472No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17890.12389762967005416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17870.12375911918411783No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17720.12272029053959531No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17700.12258178005365897No Hit
GTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGA17650.12223550383881812No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG17320.11995008082086854No Hit
ACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACA17150.11877274169040967No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17120.11856497596150516No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16940.11731838158807813No Hit
GGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCT16680.11551774527090573No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16510.11434040614044685No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16440.11385561943966968No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA16360.11330157749592433No Hit
CCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAG16330.11309381176701983No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTGCCCC15700.1087307314600252No Hit
CATCAAGGTTCAGTGGAAGTGGATTTGGGACAGATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTG15640.10831520000221617No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15510.10741488184362996No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGGTA15420.10679158465691646No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15290.10589126649833025No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15020.10402137493818969No Hit
ACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG14960.10360584348038067No Hit
ATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGAC14850.10284403580773083No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14710.10187446240617645No Hit
GTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCA14700.10180520716320829No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14670.10159744143430377No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14650.10145893094836746No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14570.1009048890046221No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14560.10083563376165393No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14480.10028159181790858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG10700.041.90368370
GTGGTAT3100.040.608391
GTATCAA30700.037.237171
GGGTTAT400.0027798934.023251
TGCGATG400.002795993133.9831910
TATCAAC34100.033.101172
ATCAACG34800.032.5274053
CAACGCA34750.032.469645
TCAACGC34750.032.3718384
AACGCAG35450.032.0202266
ACGGAAG450.00403104831.53754259
ACGCAGA36650.030.8790877
CGCAGAG37300.030.4320938
AGAGTAC38150.029.93014511
CGTGTTC10000.029.80858252
GCAGAGT38500.029.4835599
CCGTGTC10200.029.31882712
ACACCGT10150.029.2093948
CAGAGTA38950.029.14091110
CGTGTCT10400.029.08075713
Produced by FastQC (version 0.11.5)