FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170041
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT82700.7068128381825937No Hit
TTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTC68730.587415312796731No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53580.45793267073547No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43660.3731493169897465No Hit
GTAATATACAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTTGTAGATACA34610.2958016001148678No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33080.28272513527303744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32910.28127219473505627No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG32910.28127219473505627No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30510.2607600930223813No Hit
GTATATTACTGTGCGAGATGCGCTGCTCATTACTCGATTCAAAGTGACTA29860.2552047321418651No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29770.25443552832763977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29720.2540081928752924No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28930.24725629272820351No Hit
ATTGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTACTATGTAAATTGGTATCAGCAGAAG25460.21759921233529422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24930.21306945654041182No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24470.2091379703788158No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24150.20640302348379244No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23620.20187326768891004No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG23330.19939472206529515No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCTGCTCTGG23310.19922378788435618No Hit
GTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTA23050.19700164353214974No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22310.19067707883740828No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21790.18623279013299535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21200.18119023179529606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20920.17879715326215062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20710.1770023443622916No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20560.1757203380052494No Hit
ATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA20150.17221618729600072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19940.1704213783961417No Hit
ATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCT19880.1699085758533248No Hit
GCATATTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGGCTGC19420.16597708969172875No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCC19240.1644386820632781No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA18710.15990892626839573No Hit
ATATTCCTGTGAGCAGTATGATAATATGCCGATCACCTTCGGCCAAGGGA17650.15084941467863092No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCAT17510.1496528754120582No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17380.14854180323595498No Hit
CAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTCCCAGATCCACTCCCA17140.14649059306468748No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16860.14409751453154207No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16790.1434992448982557No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.14298644235543884No Hit
ATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAA16640.14221723854121351No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16340.13965322582712913No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16240.13879855492243434No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCT16000.13674734475116684No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15980.13657641057022787No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15910.13597814093694152No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15900.13589267384647205No Hit
ACATATTCCTGTGAGCAGTATGATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA15600.13332866113238767No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15080.12888437242797476No Hit
GATCTACGATGCATCGAATTTGAAAATAGGGGTCCCATCAAGGTTCAGTGGGAGTGGATCTGGGACAAATTTTA14670.1253802217187261No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14460.12358541281886704No Hit
GGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTCCCAGATCCACTCCCA14320.12238887355229432No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14250.12179060391900798No Hit
CGATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCC14200.12136326846666057No Hit
AGGTAATATCGTGACTCGCCTGGCAGGTGATGGTGACTCTGTCTCCAATAGATGCAGACAGGGAGGATGGAGA13570.11597884176708338No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13500.11538057213379702No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13480.11520963795285807No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13470.11512417086238858No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCATCCAGATGACCCAGTC13430.11478230250051066No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13340.11401309868628534No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCT13180.11264562523877368No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13130.1122182897864263No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13090.11187642142454837No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13050.11153455306267045No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13040.11144908597220099No Hit
CCCCTAAACTCCTGATCTACGATGCATCGAATTTGAAAATAGGGGTCCCA12980.11093628342938411No Hit
CATCAAGGTTCAGTGGGAGTGGATCTGGGACAAATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG12900.11025254670562826No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12810.10948334289140295No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12780.10922694161999451No Hit
GAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAG12710.10862867198670817No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCATATTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGG12620.10785946817248285No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12530.10709026435825754No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAATGCATGGCAAAGTCAC12480.10666292890591014No Hit
GCTCAGCTCCTGGGGCCCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCATCCAGATGACCCAGTCTCCATC12460.10649199472497117No Hit
ATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATC12420.10615012636309328No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12240.10461171873464264No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12230.10452625164417316No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12130.10367158073947835No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12040.10290237692525304No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12030.10281690983478357No Hit
GAGTGGATCTGGGACAAATTTTACTTTCACCATCAGCAGCCTGCAGCCTG12030.10281690983478357No Hit
GTTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTACATAGTAGGTA11980.10238957438243616No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11880.10153490347774138No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11870.1014494363872719No Hit
ACCTTGATGGGACCCCTATTTTCAAATTCGATGCATCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG11860.10136396929680241No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11770.10059476548257709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGT150.00225647269.7517549
TCGTATG605.5570126E-946.05117840
GTCACCG10300.043.11925570
CGTATGC651.1110387E-842.5786841
ACGCTCC350.001379056639.24525534
GTATCAA22050.039.205651
CGTCACG350.00139485139.15443830
ACCCGAG350.001411678339.05903221
ACCGAAC350.001413755839.04734816
TCTGGAG12000.038.06814670
CGCGACT400.001871814436.87875467
TATCAAC23900.035.4560662
GTATGCC1003.6561687E-1034.6281442
CGACTTG500.002689399834.2524369
CCGAACC501.9702956E-434.16789217
GTGGTAT1650.033.133921
ATCAACG25800.032.9773983
TTATACA1155.2750693E-1132.6837272
GATCTCG959.094947E-932.57282336
TCAACGC26600.032.2425084
Produced by FastQC (version 0.11.5)