FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170041
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT77610.6633100891336287No Hit
TTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTC61900.5290412900060767No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53490.45716346692124465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38570.32964656794078157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35710.3052029800665105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33800.28887876578683996No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG33020.28221233273022056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31710.2710161438787188No Hit
GTAATATACAGCCGTGTCTTCGGCTCTCAGGCTGTCCATTTGTAGATACA31500.2692213349788597No Hit
GTATATTACTGTGCGAGATGCGCTGCTCATTACTCGATTCAAAGTGACTA29560.25264071942778077No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28800.24614522055210034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27510.23511996588153747No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24770.21170198309290017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24530.20965077292163267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24320.2078559640217736No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG24240.20717222729801776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23690.20247153732219642No Hit
GTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTA23390.199907524608112No Hit
ATTGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTACTATGTAAATTGGTATCAGCAGAAG23320.19930925497482566No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23130.19768538025590554No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21700.18546358631877002No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCTGCTCTGG21690.18537811922830053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20940.17896808744308962No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20700.1769168772718221No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20530.17546393673384095No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19300.164951484606095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19040.16272934025388855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18790.16059266299215155No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18600.15896878827323146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18530.15837051863994508No Hit
ATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA17740.15161861849285624No Hit
ATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCT17680.15110581595003936No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCAT17590.15033661213581404No Hit
GCATATTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGGCTGC17480.14939647414064977No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCC17470.1493110070501803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17440.14905460577877186No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17330.1481144677836076No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16830.14384111326013363No Hit
CAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTCCCAGATCCACTCCCA16420.14033696255088496No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16180.1382857523796175No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15820.13520893712271623No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC15760.13469613457989935No Hit
ATATTCCTGTGAGCAGTATGATAATATGCCGATCACCTTCGGCCAAGGGA15640.13367052949426558No Hit
ATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAA15130.12931170788032215No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14850.1269186293471767No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14810.1265767609852988No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14740.12597849135201244No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCT14300.12221793937135536No Hit
ACATATTCCTGTGAGCAGTATGATAATATGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA14210.12144873555713005No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14140.12085046592384369No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14040.11999579501914891No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCCCCTGCT13810.1180300519383509No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13710.11717538103365609No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13650.1166625784908392No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13560.1158933746766139No Hit
GGAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATTTGTCCCAGATCCACTCCCA13490.11529510504332753No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCATATTATCATACTGCTCACAGGAATATGTTGCAATATCTTCAGG13380.11435496704816327No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13360.11418403286722431No Hit
GGGCTGGAGTGGGTGGGCATAATATGGTATGATTCAAGTAATCAATACTACGCAGACTCCGTGAAGGGCCGATT13240.11315842778159056No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCATCCAGATGACCCAGTC13080.11179095433407889No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12880.1100816125246893No Hit
CCCCTAAACTCCTGATCTACGATGCATCGAATTTGAAAATAGGGGTCCCA12860.10991067834375036No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12850.10982521125328087No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAATGCATGGCAAAGTCAC12840.10973974416281138No Hit
GCTCAGCTCCTGGGGCCCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCATCCAGATGACCCAGTCTCCATC12740.1088850732581166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12710.10862867198670817No Hit
GATCTACGATGCATCGAATTTGAAAATAGGGGTCCCATCAAGGTTCAGTGGGAGTGGATCTGGGACAAATTTTA12660.10820133653436076No Hit
CGATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCC12560.10734666562966597No Hit
AGGTAATATCGTGACTCGCCTGGCAGGTGATGGTGACTCTGTCTCCAATAGATGCAGACAGGGAGGATGGAGA12490.10674839599637961No Hit
ATGTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAACTCCTGATC12470.10657746181544066No Hit
GAGTCACCATCACCTGCCAGGCGAGTCACGATATTACCTACTATGTAAATTGGTATCAGCAGAAGCCAGGGAAAG12420.10615012636309328No Hit
GGCCGAAGGTGATCGGCATATTATCATACTGCTCACAGGAATATGTTGCA12340.10546638963933742No Hit
GCTCCTGGGGCCCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGGCATCCAGATGACCCAGTCTCCATCCTCC12260.10478265291558159No Hit
GTCTGCATCTATTGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACG12160.1039279820108868No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12130.10367158073947835No Hit
GAGTGGATCTGGGACAAATTTTACTTTCACCATCAGCAGCCTGCAGCCTG12100.10341517946806991No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12080.10324424528713097No Hit
CATCAAGGTTCAGTGGGAGTGGATCTGGGACAAATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG12050.10298784401572253No Hit
ACCTTGATGGGACCCCTATTTTCAAATTCGATGCATCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTG11990.10247504147290565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGCATATT11950.10213317311102772No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11900.10170583765868034No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11880.10153490347774138No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11810.10093663384445502No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11780.10068023257304658No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11750.10042383130163815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)