FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472638
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT96280.6537927175585582No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77940.5292543041806608No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76970.5226674851524951No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC66110.44892227417736064No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC57050.3874000263472761No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56880.3862456353835769No Hit
CTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA50880.34550242490007727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50170.3406811449928631No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47810.3246554822026866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG46830.318000757823715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46290.3143338688802No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA45790.3109386013399084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45790.3109386013399084No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44750.30387644485610177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44060.29919097565049935No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA43800.29742543652954767No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43570.2958636134610135No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43460.29511665460214936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42100.2858815268925561No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG40920.27786869549746784No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA40210.27304741559025364No Hit
GGCCAAAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAA39780.27012748550560284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38430.2609602631468155No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37970.25783661700974714No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGA37070.2517251354372222No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36940.25084236587674635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36410.24724338228403722No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT36040.2447308843042214No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT35690.24235419702601727No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT34570.234748797735764No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34260.2326437318607832No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32640.22164306503023823No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA32370.21980962055848074No Hit
TCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCA32360.21974171520767494No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC31900.21661806907060663No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31160.21159307311097497No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG30790.20908057513115919No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC30280.2056174022400617No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC30260.20548159153845005No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA30210.20514206478442087No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29880.2029011882078284No Hit
GTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT29840.2026295668046051No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28630.19441301935709931No Hit
CTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA27960.18986336085310848No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27890.1893880233974677No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26800.18198634015963192No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG26770.1817826241072144No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26740.1815789080547969No Hit
CTATAATTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGC26720.18144309735318523No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26710.18137519200237942No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG26700.18130728665157356No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCACTCAGAGTATTAGTAG25460.1728870231516503No Hit
ACTTATTACTGCCAACACTATAATTACTATTCTCCGGGGATGTGCAGTTT25280.17166472683714531No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24430.16589277201864952No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG24410.16575696131703788No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24260.16473838105495037No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24240.16460257035333872No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24220.16446675965172702No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23890.16222588307513455No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23840.16188635632110537No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23800.16161473491788206No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23800.16161473491788206No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23760.16134311351465874No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT23560.15998500649854208No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23530.15978129044612457No Hit
GTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG23270.15801575132517293No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22830.15502791588971626No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22830.15502791588971626No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22790.15475629448649295No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22670.15394143027682294No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22610.15353399817198796No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22380.15197217510345382No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22230.1509535948413663No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT21930.14891643431719132No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG21730.14755832730107468No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21570.14647184168818134No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT21370.14511373467206468No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG21270.14443468116400635No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC21220.1440951544099772No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21130.1434840062527247No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20900.14192218318419056No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20880.1417863724825789No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20620.1400208333616272No Hit
TTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20510.13927387450276307No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19820.13458840529716062No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19770.13424887854313144No Hit
GAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG19200.13037827354719897No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG19090.1296313146883348No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC18930.12854482907544149No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC18880.1282053023214123No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18650.12664347925287817No Hit
CCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA18530.12582861504320816No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18490.12555699363998485No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18440.12521746688595567No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18060.12263706355533403No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18020.1223654421521107No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17860.12127895653921737No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17660.11992084952310073No Hit
CACTATAATTACTATTCTCCGGGGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG17430.11835902645456657No Hit
CTACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA17410.1182232157529549No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17400.11815531040214908No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG17210.11686510873683824No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG17190.11672929803522658No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGA17070.11591443382555659No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16640.11299450374090576No Hit
TTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC16570.11251916628526495No Hit
ACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG16560.1124512609344591No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC16550.1123833555836533No Hit
GCTGATACCAGGCCAACCAGCTACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG16430.1115684913739833No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGCACAT16430.1115684913739833No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16420.11150058602317746No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT16380.11122896461995412No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT16360.11109315391834246No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16320.11082153251511911No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT16320.11082153251511911No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16300.11068572181350746No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC16270.11048200576108996No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16090.10925970944658497No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGA16030.10885227734174997No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16010.10871646664013832No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC15630.10613606330951667No Hit
GCTTGGTCCCCTGGCCAAAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAAT15440.10484586164420584No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15410.10464214559178833No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT15160.10294451182164253No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC15070.10233336366439003No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC15000.10185802620874919No Hit
AAACTGCACATCCCCGGAGAATAGTAATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTG14950.10151849945472004No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14910.1012468780514967No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC14880.1010431619990792No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATTAC14850.10083944594666171No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG14840.10077154059585589No Hit
GGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGAGTGGCCCGGCAAGTGATGGTGACT14800.10049991919263254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG200.006538577453.32094660
CGCGTAT200.007391998551.69222639
TGTCGTA200.00743527451.61596738
GTGGTAT3200.048.956371
CTCGACG1850.047.98227328
TCGACGT1900.046.74663529
GTCGTAT306.3468504E-445.94864739
CGAGACT1950.045.44892523
ACGAGAC2050.044.88247322
CGTATGC1700.044.70781341
CACGAGA2150.042.79200721
ACTCGAC2000.042.6562127
GACGTCA2100.042.36816831
CCGTTAA350.001262956439.9542750
TCGTATG1650.039.72796640
CGACGTC2250.039.50049630
ACGGTTA350.001398003739.13712332
ATCTCCG2350.039.1301910
GACTCGA2200.038.77046226
GTATCAA32350.038.320261
Produced by FastQC (version 0.11.5)