FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474298
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12400.26143901091718713No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11390.24014438180215816No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8620.18174228016985103No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7480.1577067581984322No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7090.1494840796292626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6140.1294544779864136No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6120.1290328021623536No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6050.1275569367781437No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT5660.1193342582089741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.11068990381574452No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5220.11005739007965458No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5130.10815984887138465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5060.10668398348717473No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC4950.10436476645484484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG200.00849770249.89584417
CTCGTAT901.8189894E-1242.10818539
CGTATGC1100.041.0751541
GACCGGA1153.3536344E-739.5075970
ATCTCGT1050.039.0793237
GTATCAA27800.038.161671
GGTATCA11250.037.5433241
TCGTATG1051.6370905E-1136.25511640
CGATTGT400.00310780433.25337214
ATCTCCG1500.033.24986610
ATCGGGG450.003495212632.46477557
CTAGCGC1350.032.21196728
GGATCGT552.9989425E-431.80305743
CGTCTTC1450.031.79466848
GCCGTCT1450.031.60630446
ATGCCGT1450.031.46561244
ATCAACG34200.031.3120373
TATCAAC34150.031.1631092
TCAACGC34550.031.0910934
GTAGTTT2101.2278178E-930.90729970
Produced by FastQC (version 0.11.5)