FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474298
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14420.30402826914724496No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10170.2144221565344994No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9200.19397087906759042No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7400.15602005490219228No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6300.13282788457889344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5730.12081012359318404No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5590.11785839282476417No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5390.11364163458416439No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5380.1134307966721344No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC5110.10773817304732469No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC4760.10035884612627503No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC4760.10035884612627503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG451.2509945E-436.9066284
CGGCACA400.00187792436.84920559
GGTATCA12600.036.1271971
GTATCAA26850.034.402091
CCAACCG1601.8189894E-1228.44580843
CGCATAA655.8846833E-428.34554159
ATCAACG32600.028.0196043
CGAAATT1651.8189894E-1227.93152248
CAACGCA32800.027.851695
TCAACGC32900.027.7641054
TATCAAC32950.027.7219732
AACGCAG33400.027.4508156
GTAGTTT2904.3655746E-1127.09196770
CGCAGAG33950.026.9139358
CCGAAAT1755.456968E-1226.27628947
CCGCATA709.33748E-426.19216758
AGAGTAC35050.026.07202511
CAGAGTA35250.026.01836610
ACGCAGA35750.025.7419977
GCAGAGT35800.025.6159429
Produced by FastQC (version 0.11.5)