FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206796
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTTCAGTTGCAAATACA66740.553034647115171No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC66460.5507144538099231No Hit
GTCTATTACTGTGCGAAATTATGGACTTATCACAATGATAGTAGTTCTGCCGAATACTACTTTGACTCCTGGGGC62490.5178174273033719No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46040.3815060706200551No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45890.38026310992081513No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCT44090.36534758152993546No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCTATGATTAGTA41440.34338860917669595No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39230.3250756548745604No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35260.2921786283680092No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAGGATCCCT34730.28778683389736126No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC34180.2832293113334814No Hit
CCCTATGATTAGTACTGCTGTCAGTTGAGAAACAGTAGTAGTCAGCCTCA32740.27129688862077767No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31850.26392198847195386No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30610.25364684669157006No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC30190.25016655673369814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28300.2345052519232745No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC28040.23235078671125856No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG26620.22058409209178687No Hit
ATCATAGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCC26000.215446521201595No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25250.20923171770539511No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC24470.20276832206934728No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATATGCATATTTTTTT24060.19937089615809134No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23900.19804507141223537No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG23790.1971335668994594No Hit
GATTAGTACTGCTGTCAGTTGAGAAACAGTAGTAGTCAGCCTCATCCTCC23660.19605633429345143No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC23220.1924103162423475No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCTATGATTAGTAC23080.19125021958972352No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22170.1837095913476677No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG22170.1837095913476677No Hit
GTACTAATCATAGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA21860.18114080590257178No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAGGATCCCTGAGAA21780.1804778935296438No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21680.17964925306348378No Hit
GGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGGCTGGACTGGCTGGC21040.17434595408005993No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20760.172025760774812No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20500.169871295562796No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20450.16945697532971604No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20340.16854547081694005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20170.1671367820244681No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC19890.16481658871922014No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTCACTATGGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGG19860.16456799657937216No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19770.16382222015982817No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA19720.1634078999267482No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCCTGGCACTCATTTA19080.1581046009433243No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA18950.15702736833731631No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18940.15694450429070034No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18780.15561867954484437No Hit
GACTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGG18720.15512149526514837No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC18260.15130974912081246No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT18220.15097829293434847No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAG18130.1502325165148045No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17980.14898955581556453No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17540.1453435377644606No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17500.1450120815779966No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA17350.14376912087875665No Hit
GTAGTAGTCAGCCTCATCCTCCTCCTGGGCCCCACTGACAGTCAAGGTGG17260.14302334445921266No Hit
GGATGAGGCTGACTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGGCGGAGGGACGAAG17210.14260902422613267No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16840.13954305450134075No Hit
TCTATGAGGACAACAAACGACCCTCCAGGATCCCTGAGAAGTTCTCTGGC16790.13912873426826075No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16520.1368914050096288No Hit
GCCTGAGACCTGAGGACACGGCTGTCTATTACTGTGCGAAATTATGGACT16220.13440548361114885No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCATATTGGTACCAGCAGAAG15910.13183669816605292No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15900.13175383411943692No Hit
CTATGGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGGCTGGACTGGC15840.13125664983974092No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG15720.13026228128034895No Hit
CTCCAGGATCCCTGAGAAGTTCTCTGGCTCCAGCTCAGGGACAATGGCCA15700.13009655318711696No Hit
CTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGGCG15520.128605000348029No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15400.12761063178863702No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15350.12719631155555702No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15340.12711344750894102No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15260.12645053513601304No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAA15130.12537330253000506No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15080.12495898229692509No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC14940.12379888564430111No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14810.12272165303829313No Hit
GTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14640.12131296424582116No Hit
GCTCAGGGACAATGGCCACCTTGACTGTCAGTGGGGCCCAGGAGGAGGATGAGGCTGACTACTACTGTTTCTCAA14530.12040145973304518No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATATGCATATT14320.11866131475410922No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14280.11832985856764523No Hit
GGGCTGGACTGGCTGGCAGTTATATCATATGATGGAGATAAAACAGACCA14250.11808126642779725No Hit
GTCCAGCCCCTTGCCTGGAGCCTGGCGCACCCAGTGCATGCCATAGTGAC14200.11766694619471725No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14140.11716976191502126No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACAC13870.1149324326563893No Hit
GCCTCATCCTCCTCCTGGGCCCCACTGACAGTCAAGGTGGCCATTGTCCC13840.11468384051654132No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13600.11269510339775737No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13590.11261223935114138No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGG13550.11228078316467738No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13410.1111206865120534No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT13380.11087209437220541No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13340.11054063818574142No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG13040.10805471678726147No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12990.10764039655418148No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12930.1071432122744855No Hit
GTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12920.10706034822786949No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC12900.1068946201346375No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTCACTATGGC12870.10664602799478952No Hit
CCTTGACTGTCAGTGGGGCCCAGGAGGAGGATGAGGCTGACTACTACTGT12830.10631457180832551No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC12780.10590025157524553No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAGGCTAACCGTGGACAAGAG12690.10515447515570153No Hit
GACCTACACCTGCAATGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAAATATGGTCC12690.10515447515570153No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA12600.10440869873615756No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12330.1021713694775256No Hit
GGGTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12200.10109413687151765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTGT12250.045.5574270
ACCGTAC704.6929927E-1044.09477227
GGTATCA5800.043.727871
TCGTATG758.60382E-1041.45596340
CGTACTA801.7353159E-938.5925329
GACCGTA801.7425918E-938.5765326
GTATCAA12550.036.5944861
CCCACGA853.1704985E-936.28475619
AATAGAC16450.036.2525023
TAGACAG16550.035.8263635
ATAGACA16650.035.611194
CGTGTCC16750.035.6002513
CCGTGTC17000.035.07671712
CAGCCGT16950.034.97519
TCAGGTC17000.034.672120
TAATAGA17000.034.47482
GTAATAG17150.034.3731161
GCCGTGT17350.034.17017711
GTTGCAA17500.033.92748339
AATTGTC18000.033.5942363
Produced by FastQC (version 0.11.5)