FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206796
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC66380.5500515414369952No Hit
GTCTATTACTGTGCGAAATTATGGACTTATCACAATGATAGTAGTTCTGCCGAATACTACTTTGACTCCTGGGGC60840.5041448596117323No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGGCTGTTCAGTTGCAAATACA60340.5000016572809323No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT46430.384737768438079No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43750.3625302039449915No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCT41090.340488367545136No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39610.3282244886459683No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCTATGATTAGTA37490.3106573107633767No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34490.2857980967785773No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34200.28339503942671335No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAGGATCCCT33140.2746114504854176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC30240.2505808769667781No Hit
CCCTATGATTAGTACTGCTGTCAGTTGAGAAACAGTAGTAGTCAGCCTCA30020.2487578679412262No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28840.23897991044053843No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27980.23185360243156258No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27510.22795899224061067No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26660.22091554827825083No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG24740.2050056513279792No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23700.19638779047991542No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAGGATCCCTGAGAA23610.19564201406037143No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23310.1931560926618915No Hit
ATCATAGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCC23220.1924103162423475No Hit
GATTAGTACTGCTGTCAGTTGAGAAACAGTAGTAGTCAGCCTCATCCTCC22480.18627837679276366No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC22370.1853668722799877No Hit
GTACTAATCATAGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA22340.18511828014013967No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG22210.1840410475341317No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22030.18254949469504372No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG21580.17882061259732382No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21330.17674901143192387No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC21250.1760860990589959No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATATGCATATTTTTTT21220.1758375069191479No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20300.16821401463047608No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA19870.16465086062598816No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19820.16423654039290816No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19550.1619992111342762No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTCACTATGGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGG19320.16009333806210826No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19310.16001047401549226No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT19260.1595961537824123No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCTATGATTAGTAC19120.1584360571297883No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19030.1576902807102443No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18980.15727596047716433No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18640.1544585828922204No Hit
GACTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGG18630.1543757188456044No Hit
GGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGGCTGGACTGGCTGGC18620.1542928547989884No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18440.1528013019599004No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCAG18430.15271843791328443No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18420.15263557386666843No Hit
GGATGAGGCTGACTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGGCGGAGGGACGAAG18380.15230411768020446No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC18370.15222125363358843No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCCTGGCACTCATTTA18280.15147547721404445No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA18020.14932101200202852No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA17960.14882382772233252No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17160.14219470399305267No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC17060.1413660635268927No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACAC16540.1370571331028608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16190.13415689147130086No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAA16130.1336597071916049No Hit
GTAGTAGTCAGCCTCATCCTCCTCCTGGGCCCCACTGACAGTCAAGGTGG16080.1332453869585249No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16040.1329139307720609No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15960.13225101839913292No Hit
TCTATGAGGACAACAAACGACCCTCCAGGATCCCTGAGAAGTTCTCTGGC15480.128273544161565No Hit
GGGCTGGACTGGCTGGCAGTTATATCATATGATGGAGATAAAACAGACCA15470.128190680114949No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15450.12802495202171701No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCATATTGGTACCAGCAGAAG15420.127776359881869No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC15390.12752776774202101No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15390.12752776774202101No Hit
GGGTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15290.12669912727586105No Hit
GTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15270.12653339918262904No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15240.12628480704278106No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15060.1247932542036931No Hit
GCCTGAGACCTGAGGACACGGCTGTCTATTACTGTGCGAAATTATGGACT14830.12288738113152511No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG14740.12214160471198114No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14590.12089864401274117No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14540.12048432377966117No Hit
CTCCAGGATCCCTGAGAAGTTCTCTGGCTCCAGCTCAGGGACAATGGCCA14430.1195728192668852No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14330.11874417880072524No Hit
CTATGGCATGCACTGGGTGCGCCAGGCTCCAGGCAAGGGGCTGGACTGGC14220.11783267428794925No Hit
CTACTACTGTTTCTCAACTGACAGCAGTACTAATCATAGGGTGTTCGGCG14160.11733549000825325No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13980.1158439371691653No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13880.11501529670300531No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13600.11269510339775737No Hit
GTCCAGCCCCTTGCCTGGAGCCTGGCGCACCCAGTGCATGCCATAGTGAC13520.11203219102482939No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGG13510.11194932697821337No Hit
GCTCAGGGACAATGGCCACCTTGACTGTCAGTGGGGCCCAGGAGGAGGATGAGGCTGACTACTACTGTTTCTCAA13370.11078923032558942No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13270.10996058985942943No Hit
GCCTCATCCTCCTCCTGGGCCCCACTGACAGTCAAGGTGGCCATTGTCCC13140.10888335725342145No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13070.10830330892710946No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAGGCTAACCGTGGACAAGAG13040.10805471678726147No Hit
CCTTGACTGTCAGTGGGGCCCAGGAGGAGGATGAGGCTGACTACTACTGT12920.10706034822786949No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC12910.1069774841812535No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12880.10672889204140551No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12820.10623170776170951No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATATGCATATT12810.10614884371509352No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12780.10590025157524553No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12600.10440869873615756No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12570.10416010659630957No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCATATTGGTACCAGCAGAAGTCAGGCCAGGCCC12540.10391151445646157No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12530.10382865040984557No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC12400.10275141780383759No Hit
GACCTACACCTGCAATGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGTCCAAATATGGTCC12370.1025028256639896No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12300.10192277733767761No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12280.10175704924444563No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12270.10167418519782963No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12210.10117700091813364No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG12190.10101127282490165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)