EGAF00004687445 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004687445
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1825
Sequences flagged as poor quality	0
Sequence length	21-76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	606	33.205479452054796	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	74	4.054794520547945	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	60	3.287671232876712	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGT	34	1.8630136986301369	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATGTCGTATGCCGT	22	1.2054794520547945	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT	16	0.8767123287671232	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAGCTCGTATGCCGT	13	0.7123287671232876	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	12	0.6575342465753425	RNA PCR Primer, Index 29 (95% over 23bp)
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC	11	0.6027397260273972	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT	10	0.547945205479452	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGG	9	0.4931506849315068	No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCG	9	0.4931506849315068	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG	8	0.4383561643835616	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTACTCTCGTATGCCGT	7	0.3835616438356164	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCC	6	0.32876712328767127	No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCT	6	0.32876712328767127	RNA PCR Primer, Index 29 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATTCCGT	6	0.32876712328767127	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5	0.273972602739726	No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCG	5	0.273972602739726	No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	5	0.273972602739726	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTAGTATGCCGT	4	0.2191780821917808	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCG	4	0.2191780821917808	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGG	3	0.16438356164383564	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGG	3	0.16438356164383564	No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA	3	0.16438356164383564	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTG	3	0.16438356164383564	Illumina PCR Primer Index 10 (96% over 25bp)
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC	3	0.16438356164383564	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	3	0.16438356164383564	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGC	3	0.16438356164383564	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATGTCGTATGCCG	3	0.16438356164383564	No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	3	0.16438356164383564	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATGTCGTATGCC	3	0.16438356164383564	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGT	3	0.16438356164383564	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	3	0.16438356164383564	RNA PCR Primer, Index 29 (96% over 30bp)
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCT	2	0.1095890410958904	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCG	2	0.1095890410958904	No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT	2	0.1095890410958904	No Hit
CATATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT	2	0.1095890410958904	No Hit
CTGGTAGCGCTAGCAAATCCTCCAATG	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGT	2	0.1095890410958904	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTC	2	0.1095890410958904	No Hit
GTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAG	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCAGT	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGGCGT	2	0.1095890410958904	No Hit
CAGGTGTGTCGCCCTTACCACTCCCAGACTTTCGACGGTGTAACCACCGCAGGCAGTGGGATATCC	2	0.1095890410958904	No Hit
GTCCTTCAGTAACCCCTCTCATTAATTCTTTGTGCAAACAAACTCTAGTT	2	0.1095890410958904	No Hit
GTGTAAAGTGAGCTACCATAGTGCTGACAGTAATACACTGCAAAATCTTC	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCCT	2	0.1095890410958904	No Hit
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGC	2	0.1095890410958904	No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	2	0.1095890410958904	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGC	2	0.1095890410958904	No Hit
ATTGGAGGATTTGCTAGCGCTACCAGTGG	2	0.1095890410958904	No Hit
CACTGGTAGCGCTAGCAAATCCTCCAAT	2	0.1095890410958904	No Hit
CCACTGGTAGCGCTAGCAAATCCTCC	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATGTCGTATGGCGT	2	0.1095890410958904	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATTCCG	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGG	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGT	2	0.1095890410958904	No Hit
ACGAAGGACTTGGCACGAGTTTTTTGTATCCAGGAGGAAGCCATAACGGTGAAACTGGACGGTCTTCCAAAACT	2	0.1095890410958904	No Hit
GGTCTCTCGTGCTCTCCGTGGTGCGACTTCAGGTACACATTCAGCATGCT	2	0.1095890410958904	No Hit
CCCCAACGTTGGCTGACCGCGGTTTGCTGTGGTACGGTGAATTTCACCAA	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATGTCGTATGCCGG	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAGCGCGTATGCCGT	2	0.1095890410958904	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCC	2	0.1095890410958904	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAAGCCGT	2	0.1095890410958904	No Hit
CTCACGTACAGAGGGGTAAGGATAAGAATTACTTAAGTCTACTCTTTGTA	2	0.1095890410958904	No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATC	2	0.1095890410958904	No Hit
GTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGA	2	0.1095890410958904	No Hit
CATTGGAGGATTTGCTAGCGCTACCAG	2	0.1095890410958904	No Hit
ACAGGCGCCCACCACCACGCCTGGCTAATTTTTTGTACTTTTAGTAGAGA	2	0.1095890410958904	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTACTCTCGTATGCCGTCTT	2	0.1095890410958904	No Hit
CACTGGTAGCGCTAGCAAATCCTCCAA	2	0.1095890410958904	No Hit
GAGGAACGGAGGGAAAGACAGAGCGACGCAGGGACTGGGGGCGGGCGGGA	2	0.1095890410958904	No Hit
TCATTGGAGGATTTGCTAGCGCTACCAG	2	0.1095890410958904	No Hit
CAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATG	2	0.1095890410958904	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2	0.1095890410958904	Illumina PCR Primer Index 10 (96% over 29bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	60	0.0	73.15476	70
CTCTTAT	25	2.0411174E-4	54.622223	1
TCTCGTA	95	0.0	51.747368	38
CTAATCT	95	0.0	51.747368	34
CTCGTAT	95	0.0	51.747368	39
AATCTCG	90	0.0	50.720634	36
ATCTCGT	90	0.0	50.720634	37
TAATCTC	90	0.0	50.720634	35
TATACAC	115	0.0	50.466187	3
TTATACA	115	0.0	50.466187	2
TTCTGCT	70	1.8189894E-12	50.163265	52
TCGTATG	105	0.0	50.163265	40
CTTATAC	120	0.0	48.363422	1
CCGTCTT	95	0.0	48.05113	47
GCCGTCT	95	0.0	48.05113	46
AAGGGGG	80	1.8189894E-12	48.007812	69
GTCTTCT	90	0.0	46.81905	49
CGTCTTC	90	0.0	46.81905	48
CTTCTGC	90	0.0	46.81905	51
TCTGCTT	75	1.8189894E-12	46.819046	53

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content