FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014013
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46880.46232148897499337No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46570.4592643289583072No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35920.35423608967537895No Hit
GGCCAGGGCAGTCTCCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCC34480.34003508830754636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32330.3188322043208519No Hit
CTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA31130.3069980365143248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28800.2840200273566513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28450.28056839507974746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27530.2714955330947434No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27310.26932593566354673No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26690.26321161563017437No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA25190.24841890587201546No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24580.24240320390369752No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24240.23905018969184816No Hit
GGTCTAGTCAGAGTCTCCTCCATAGTAATGGATACAATTATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTC24120.2378667729111954No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24070.23737368258592348No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGC22790.22475057025896117No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21420.21123989534650936No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21120.2082813533948776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20910.20621037402873532No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20790.20502695724808262No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20580.20295597788194036No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC19920.19644718558835045No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19860.1958554771980241No Hit
CCCCTGGAGAGCCGGCCTCCATCTCGTGCAGGTCTAGTCAGAGTCTCCTC19230.18964253909959733No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC19090.18826188618883583No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18970.18707846940818312No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17780.1753429196667104No Hit
GTCTCCACCCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCGT17780.1753429196667104No Hit
GTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA17580.1733705583656225No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATAATTGTATCCATTACTATGG17320.17080648867420833No Hit
ATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTC17190.1695244538285012No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16540.16311427959996566No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16270.16045159184349708No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAAAATCAGCAGGG16000.15778890408702848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15940.15719719569670212No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15930.15709857763164772No Hit
CTGCAGGTACCAATCCAAATAATTGTATCCATTACTATGGAGGAGACTCTGACTAGACCTGCACGAGATGGAGGC14880.14674368080093647No Hit
GACTAGACCTGCACGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG14580.1437851388493047No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14450.14250310400359759No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14300.14102383302778168No Hit
CTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACG14290.1409252149627273No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13880.1368818742954972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13880.1368818742954972No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13720.13530398525462692No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13660.13471227686430057No Hit
CTCCAGGGGTGACGGGCAGGGAGGGTGGAGACTGAGTCATCACAATATCC13580.13392333234386541No Hit
CAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA13580.13392333234386541No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13530.13343024201859346No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13520.13333162395353906No Hit
CCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGC13470.13283853362826709No Hit
GGTTGTAGAGATTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAAT13380.13195097104277756No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13230.13047170006696165No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGA13200.13017584587179848No Hit
TTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA13090.12909104715620015No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT13010.12830210263576503No Hit
GTTGTAGAGATTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAAT13000.12820348457071062No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12920.12741454005027547No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12840.12662559552984035No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12840.12662559552984035No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12820.12642835939973157No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12800.1262311232696228No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12610.1243573800335893No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12580.12406152583842613No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12480.12307534518788221No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12460.12287810905777342No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12430.12258225486261023No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12420.12248363679755585No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCT12340.12169469227712071No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12290.12120160195184876No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12280.12110298388679436No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12240.12070851162657677No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12230.12060989356152238No Hit
GCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACCCTCCCTGCCCGTCACCC12190.12021542130130482No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11940.117749969674945No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11920.1175527335448362No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11750.11587622643891152No Hit
GGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA11710.11548175417869395No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACCCTCC11660.114988663853422No Hit
GTTCTAATCTGGCCTCCGGGGTCCCTGCCAGGTTCAGTGGCAGTGGATCA11080.10926881608026723No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11010.10857848962488646No Hit
GGCACAGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAATCTCTA10940.10788816316950572No Hit
CCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCACTGAACCTGG10600.10453514895765637No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10580.10433791282754759No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10550.10404205863238439No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10500.10354896830711242No Hit
GCAGAGGCCAGGGCAGTCTCCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGCCAGGTT10480.10335173217700365No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10470.10325311411194926No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10360.10216831539635093No Hit
CCTCCATAGTAATGGATACAATTATTTGGATTGGTACCTGCAGAGGCCAG10240.10098489861569823No Hit
CTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGCCAGGTT10150.10009733603020868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG200.00795431150.74127210
GTATTGG352.5502284E-548.3345531
GGTATCA8400.046.3206181
CGTATGC2500.044.23050341
ACGAGAC2600.041.65440422
CTTATAC2550.041.131761
TCGTATG2700.040.8759340
TATACAC2650.040.8543623
GTATGCC2800.039.57124742
GGCAATC2550.038.8343433
CCCACGA2800.038.66382619
TCTCCGA2750.038.13283511
GTATCAA21700.037.5762181
TTATACA2800.037.457432
ATGCCGT3000.037.0453544
CGAGACA2950.036.71779323
ATCTCCG3000.036.08268410
TACACAT3300.034.8577465
AGGCAAT3100.034.1117832
GCCCACG3200.033.82751518
Produced by FastQC (version 0.11.5)