FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014013
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44010.43401810430438265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39590.3904289195503411No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36410.35906837486304416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33490.3302718998671615No Hit
GGCCAGGGCAGTCTCCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCC32540.32090318368699416No Hit
CTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA27600.2721858595501241No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27450.2707065885743082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26880.2650853588662078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26030.25670282333658445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25310.24960232265266816No Hit
GGTCTAGTCAGAGTCTCCTCCATAGTAATGGATACAATTATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTC24960.24615069037576442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24930.24585483618060122No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA24910.24565760005049245No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24430.2409239329278816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22110.21804454183526248No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22100.21794592377020805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22070.2176500695750449No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22010.21705836118471855No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21500.2120288398669445No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21260.20966200630563908No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21180.20887306178520396No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGC20920.20630899209378972No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19660.19388311589693624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19250.18983977522970613No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19130.1886563584490534No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC18560.183035128740953No Hit
ATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTC17930.17682219064252627No Hit
GTCTCCACCCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCGT17770.175244301601656No Hit
CCCCTGGAGAGCCGGCCTCCATCTCGTGCAGGTCTAGTCAGAGTCTCCTC17710.17465259321132964No Hit
GTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA17290.17051063447904516No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC17040.16804518285268533No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16960.16725623833225017No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16720.16488940477094474No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16570.16341013379512886No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16510.16281842540480249No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAAAATCAGCAGGG16510.16281842540480249No Hit
GGTTGTAGAGATTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAAT16210.1598598834531707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15900.15680272343648455No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15810.155915160850995No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATAATTGTATCCATTACTATGG15580.153646945354744No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15350.15137872985849293No Hit
GGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA15180.14970222275256825No Hit
CTGCAGGTACCAATCCAAATAATTGTATCCATTACTATGGAGGAGACTCTGACTAGACCTGCACGAGATGGAGGC14300.14102383302778168No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.1408265968976729No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14040.13845976333636748No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13770.13579707557989887No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13760.1356984575148445No Hit
GACTAGACCTGCACGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG13400.13214820717288633No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13290.131063408457288No Hit
CTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACG13200.13017584587179848No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13100.12918966522125455No Hit
GTTGTAGAGATTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAAT13090.12909104715620015No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13040.1285979568309282No Hit
CTCCAGGGGTGACGGGCAGGGAGGGTGGAGACTGAGTCATCACAATATCC12930.12751315811532987No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12830.12652697746478597No Hit
TTATTACTGCATGCAATCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA12670.12494908842391568No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12650.12475185229380689No Hit
CCCCAACTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGC12320.12149745614701193No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12300.12130022001690315No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12200.1203140393663592No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGA12190.12021542130130482No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12150.11982094904108725No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12140.11972233097603285No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12030.11863753226043453No Hit
CAGTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA11980.11814444193516258No Hit
GCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACCCTCCCTGCCCGTCACCC11870.11705964321956425No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11870.11705964321956425No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11840.11676378902440107No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11800.11636931676418351No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11800.11636931676418351No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11770.11607346256902033No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11720.11558037224374834No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11570.11410110126793245No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11490.11331215674749733No Hit
CTCCTGATCTATTTGGGTTCTAATCTGGCCTCCGGGGTCCCTGCCAGGTT11260.1110439412512463No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10990.10838125349477767No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10930.10778954510445134No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAATCTCTACAACCT10910.10759230897434255No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACCCTCC10860.10709921864907057No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10790.10640889219368983No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10770.10621165606358104No Hit
GTTCTAATCTGGCCTCCGGGGTCCCTGCCAGGTTCAGTGGCAGTGGATCA10760.10611303799852666No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10720.10571856573830907No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10530.1038448225022756No Hit
CCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCACTGAACCTGG10430.10285864185173169No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10280.1013793708759158No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10170.10029457216031747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT754.0370578E-544.71370370
GTGGTAT2900.041.9092561
TGGTATC3050.039.838322
GGTATCA8250.039.2846371
GTATCAA20550.035.8137781
GTAGTTT1359.1320544E-734.77732570
TATCAAC22700.031.6702962
AATGGTA4100.031.08147670
TACGTAC1101.3205863E-930.72142812
ATACGTA1101.3224053E-930.71991211
ATCAACG23500.030.4470333
CAACGCA23700.030.3354915
TCAACGC23800.030.2050534
AACGCAG23900.030.0875726
GTGCTAG1351.2732926E-1130.0090981
CGCAGAG24550.029.1663888
ACGCAGA24750.029.0614327
AGAGTAC25450.028.28163311
GAGTTTA3350.028.217511
CAGAGTA25550.028.1667710
Produced by FastQC (version 0.11.5)