FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161353
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCC40270.34675072953701414No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36790.3167856801506519No Hit
CCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC33890.2918148056620166No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAG31670.2726991707086476No Hit
GTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAG31060.2674466764196588No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30230.26029983992808386No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27780.23920375630837482No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24920.2145773076747552No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAAT24920.2145773076747552No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23590.2031251479954846No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23400.20148912518416018No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22450.19330901112753832No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21710.18693713280974864No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20660.1778959541155876No Hit
GATTATTACTGCTGCTCACATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20020.17238514043533706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19710.1697158400589657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18990.16351617466868384No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18390.15834978684344897No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18110.15593880585833936No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17340.14930860814928795No Hit
TCTCTGGGCTCCAGGCTGAGGATGAAGCTGATTATTACTGCTGCTCACAT17050.1468115207004244No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16990.14629488191790094No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16970.1461226689903931No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16650.14336726215026782No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16620.1431089427590061No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16540.14242009104897477No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16510.14216177165771304No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG16420.1413868134839278No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16310.14043964238263473No Hit
GTAATAATCAGCTTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16290.1402674294551269No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTG16090.13854530018004863No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCAC15880.13673706444121642No Hit
CATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15490.13337891235481375No Hit
GTCAGTCACCATCTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCA15160.13053739905093456No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14460.12450994658816053No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14010.12063515571923437No Hit
CTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGC13830.11908523937166392No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13710.11805196180661694No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13700.11796585534286302No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC13340.1148660226477221No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13200.11366053215516728No Hit
GCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCA13140.1131438933726438No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13070.11254114812636641No Hit
CCCCTGAGGGCCGCTTAGTGACATCATACATCTTGAGTTTGGGGGCTTTGCCGGGGTGCTGTTGGTACCAGGAGA12780.11004406067750287No Hit
GTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12450.1072025473736237No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11940.10281111772217405No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAGCA11810.10169173369337316No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCT11800.10160562722961926No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11710.10083066905583402No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11650.10031403027331053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG200.0076179251.29939736
ATGCCGT2550.047.69571744
GCCGTCT2300.047.04088646
AATCTCG2700.046.86610836
TCGTATG2500.046.8568140
CGTATGC2600.045.1558141
GGTATCA12500.044.8362921
GAGACGC3600.044.09940724
CGAGACG3650.043.4896823
GTGGTAT3300.042.9701651
ATCTCGT2800.042.80923537
TGGTATC3400.042.6993452
CGCTCAT3650.042.6764928
ACGAGAC3750.042.3226722
ACGCTCA3800.040.0748127
GACGCTC3900.039.88742426
GTATGCC3150.039.56844342
GAATCTC3200.039.4864535
CACGAGA3850.039.46749521
CCCACGA3950.039.31978219
Produced by FastQC (version 0.11.5)