FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161353
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCC35840.30860556609403No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35060.30188926192122467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32740.2819125623303164No Hit
CCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC32620.28087928476526947No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29350.25272247111773943No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27780.23920375630837482No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAG27310.23515675251194082No Hit
GTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAG26430.22757938370159633No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23350.20105859286539066No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAAT22330.19227573356249134No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22320.19218962709873744No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21680.18667881341848688No Hit
GATTATTACTGCTGCTCACATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20160.17359063092789187No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20090.17298788568161444No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19980.1720407145803214No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19400.16704653968259436No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19100.1644633457699769No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18980.16343006820492995No Hit
TCTCTGGGCTCCAGGCTGAGGATGAAGCTGATTATTACTGCTGCTCACAT17040.14672541423667051No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCAC16550.1425061975127287No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16480.1419034522664513No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16080.1384591937162947No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15860.1365648515137086No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15710.13527325455739986No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG15620.13449829638361463No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15530.1337233382098294No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15260.13139846368847372No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15190.13079571844219628No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14900.12829863099333277No Hit
CATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14880.12812641806582495No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTG14610.12580154354446926No Hit
GTAATAATCAGCTTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14480.12468215951566837No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14310.12321834963185181No Hit
GTCAGTCACCATCTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCA14240.12261560438557441No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC13990.12046294279172655No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13940.12003241047295696No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13540.11658815192280038No Hit
CTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGC13090.11271336105387424No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12380.1065998021273463No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12180.10487767285226801No Hit
GTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12140.10453324699725235No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAGCA12050.10375828882346712No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11860.10212226601214273No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11840.10195005308463491No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11680.10057234966457226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA100.0069687357145.8387970
ACGCGTA200.00806229950.56892419
GCGGCAT604.804395E-448.61293870
GGTATCA15150.042.5518721
GTGGTAT4350.041.8988841
GTATCAA35400.035.5634161
TGGTATC5050.035.390712
ATTGTAC701.1685879E-633.7039073
CGCGGTA3100.032.9313470
TCTCGCG4450.031.13412770
GTACGGT553.7403966E-430.63199813
ATCAACG42050.029.6562293
TCAACGC42700.029.1183344
CAACGCA43250.028.825955
AACGCAG43900.028.321176
TGGTCAG22300.027.46739870
TCCGCGG3350.027.20443568
TAGTACG500.00867890226.956164
ACGCAGA47150.026.5834037
CGCAAAT550.009658460526.3711761
Produced by FastQC (version 0.11.5)